Nogl_00400 : CDS information

Location

Organism	Streptomyces nogalater
Strain	ATCC 27451 (=NBRC 13445)
Entry name	Nogalamycin
Contig	AJ224512
Start / Stop / Direction	41,002 / 41,739 / + [in whole cluster] 12,300 / 13,037 / + [in contig]
Location	41002..41739 [in whole cluster] 12300..13037 [in contig]
Type	CDS
Length	738 bp (245 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative NDP-hexose N-methyltransferase
Product (GenBank)	SnogX
Gene
Gene (GenBank)	snogX
EC number	2.1.1.-
Keyword	nogalamine
Note
Note (GenBank)
Reference	ACC O54260 PmId [8668120] A gene cluster involved in nogalamycin biosynthesis from Streptomyces nogalater: sequence analysis and complementation of early-block mutations in the anthracycline pathway. (Mol Gen Genet. , 1996) [11683270] The entire nogalamycin biosynthetic gene cluster of Streptomyces nogalater: characterization of a 20-kb DNA region and generation of hybrid structures. (Mol Genet Genomics. , 2001) comment [PMID: 8668120](1996) S. nogalaterのanthracycline生合成gene clusterを解析。 11kb領域に11ORFs(snoEDCBA123XYZ)が同定されている。 SnoX: Methylase 配列解析のみ。 -- [PMID: 11683270](2001) S. nogalater genomic DNA fragmentの配列解析と、異種性発現による産物解析。 nogalamycin生合成に関する20ORFsが明らかになった。配列解析からN-methyltransferase_snogAを同定。 [PMID: 8668120]で同定されているsnogX(snoX)と共にnogalamineのamino基をdimethylateするのかもしれないと言っているが、他のhomologuesでは1geneがdimethylateを担っていることも気にしている。
Related Reference	ACC Q9ZGH6 NITE Pikro_00100 PmId [9770448] A gene cluster for macrolide antibiotic biosynthesis in Streptomyces venezuelae: architecture of metabolic diversity. (Proc Natl Acad Sci U S A. , 1998) [12119032] Expression, purification, and characterization of two N,N-dimethyltransferases, tylM1 and desVI, involved in the biosynthesis of mycaminose and desosamine. (Biochemistry. , 2002) comment Blast 10th, id56%, 1e-70 Streptomyces venezuelae_desVI N,N-dimethyltransferase --- [PMID: 9770448](1998) 12員環骨格を持つMethymycin and Neomethymycinと、14員環骨格を持つNarbomycin and Pikromycinの生合成で共有されるclusterの同定。 DesVI: N,N-Dimethyltransferase desI, desII, desIII, desIV, desV, desVI, and desVIII はdeoxysugar生合成を担う。 desVI のDeletion/replacement mutationにより、methymycin, neomethymycin, narbomycin, and pikromycinは非産生。それらのaglyconeである10-deoxymethynolide and narbonolideを蓄積。 --- [PMID: 12119032](2002)abstract N,N-dimethyltransferasesであるtylM1 and desVI の過剰発現、精製、触媒機能確認。 TylM1 and DesVI はhomodimeric proteinで、ほとんど同じ生化学的特性を持つ。

Sequence

>putative NDP-hexose N-methyltransferase [SnogX]
MTRVYGTELTQIYELVHEGRGKDYGAEAEEITRRIRARLPGARTLLDVACGTGAHLRAFA
TRFEEVEGVELSEAMCAVARRRLPGVALHRADMRDFRLGRTFHAVTCMFGSIGYARTPQE
LTATLRCFAAHLAPGGVAAVDPWWFPETYLDGYVSGDTMTVDGRTVSRVSHSTREGTASV
MRVHYLVADAALGVRHFSESHRISLFSREQYEEAFSRAGFAVEYVPRLHAGRGLFLGVRK
SGRAG

>putative NDP-hexose N-methyltransferase [SnogX]
GTGACGCGTGTGTACGGCACGGAACTCACACAGATCTACGAACTGGTCCACGAGGGCCGC
GGCAAGGATTACGGAGCGGAGGCCGAGGAGATCACCCGCCGGATCCGGGCGCGGTTGCCC
GGGGCGCGGACGCTGCTCGATGTCGCCTGCGGCACGGGCGCGCACCTGCGGGCGTTCGCG
ACGCGATTCGAGGAGGTGGAGGGGGTGGAACTGTCCGAGGCGATGTGCGCGGTCGCGCGC
CGCCGCCTGCCCGGAGTGGCCCTGCACCGGGCGGACATGCGCGACTTCCGGCTCGGCCGG
ACGTTCCACGCCGTCACCTGCATGTTCGGCTCGATCGGCTACGCCCGTACGCCGCAGGAG
CTCACGGCGACGCTGCGCTGCTTCGCCGCGCACCTGGCGCCCGGCGGGGTGGCCGCGGTC
GACCCGTGGTGGTTCCCGGAGACGTACCTGGACGGGTATGTCTCGGGCGACACCATGACC
GTGGACGGGCGGACGGTCTCCCGCGTCTCCCACTCGACGCGCGAGGGCACCGCCTCGGTG
ATGCGGGTGCACTACCTGGTGGCGGACGCCGCCTTGGGCGTGCGCCACTTCTCGGAGTCG
CACCGCATCTCGCTGTTCTCCCGCGAGCAGTACGAGGAGGCCTTCTCGCGGGCGGGGTTC
GCGGTGGAGTACGTCCCGCGGCTGCACGCGGGGCGCGGACTGTTCCTCGGTGTCCGGAAG
TCCGGGCGGGCGGGGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	No significant hit
SignalP	Eukaryota Bacteria, Gram-positive
TMHMM	No significant hit

Nogl_00390 Nogl_00410