Nogl_00440 : CDS information

Location

Organism	Streptomyces nogalater
Strain	ATCC 27451 (=NBRC 13445)
Entry name	Nogalamycin
Contig	AJ224512
Start / Stop / Direction	47,108 / 48,172 / + [in whole cluster] 18,406 / 19,470 / + [in contig]
Location	47108..48172 [in whole cluster] 18406..19470 [in contig]
Type	CDS
Length	1,065 bp (354 aa)

Annotation

Category
Product
Product (GenBank)	SnoP
Gene
Gene (GenBank)	snoP
EC number
Keyword
Note	This ORF is not involved in nogalamycin biosynthesis.
Note (GenBank)
Reference	ACC Q9RIP7 PmId [22120896] Identification of late-stage glycosylation steps in the biosynthetic pathway of the anthracycline nogalamycin. (Chembiochem. , 2012) comment aglycone生合成とdeoxysugar形成に必要な29ORFsを含むpSnogaori + pIJTZOMLTN を異種ホストS. albusに導入したときの主要産物はnogalamycinであった。この導入されたgenesにsnoP (putative lipoate protein ligase)は含まれないので、nogalamycin生合成には必要ない。

Sequence

>[SnoP]
MHGEYKVPGGKLVVVDVDVEDGVLRHVRVAGDFFLEPDEALDAVNGALEGAPADTDAAGL
AARIDAALPAGTVMYGLTSEGVGIAVRRALARATDWTDYDWQLIHEAPQSPALHMALDEV
LTAEVAAGRRPPTLRVWEWGAPAVIIGSFQSLRNEVDAEGAARHGVEVVRRISGGGAMFV
EPGNTITYSLSVPESLVQGLSFQDSYAYLDDWVLGALGDLGIRAWYQPLNDIATDQGKIA
GAAQKRVVGPGGGPGAVLHHVTMSYDIDADKMVEVLRIGREKLSDKGTKSAKKRVDPLRR
QTGLPREAVIDRMIESFRARYGLAEGKVTDEELARAEELARTKFGSTEWTARVP

>[SnoP]
GTGCACGGTGAGTACAAGGTGCCCGGCGGCAAGCTCGTCGTCGTGGATGTGGACGTCGAG
GACGGCGTGCTGCGGCACGTGCGGGTGGCGGGTGACTTCTTCCTGGAGCCCGACGAGGCG
CTGGACGCGGTGAACGGCGCCCTGGAGGGCGCTCCTGCGGACACGGACGCGGCGGGGCTG
GCCGCGCGGATCGACGCGGCGCTGCCCGCGGGGACGGTGATGTACGGCCTGACCTCGGAA
GGGGTGGGCATCGCGGTGCGCCGGGCGCTGGCGCGCGCCACGGACTGGACCGACTACGAC
TGGCAGCTGATCCACGAGGCCCCGCAGTCGCCGGCCCTGCACATGGCGCTGGACGAGGTG
CTGACCGCCGAGGTGGCAGCGGGCCGGCGGCCGCCGACGCTGCGGGTGTGGGAGTGGGGC
GCCCCGGCCGTGATCATCGGCAGCTTCCAGTCGCTGCGCAACGAGGTCGACGCGGAGGGC
GCCGCCCGGCACGGCGTCGAGGTGGTGCGGCGGATCTCCGGCGGCGGCGCGATGTTCGTG
GAGCCCGGCAACACGATCACCTACTCGCTGTCGGTGCCCGAGTCCCTCGTCCAGGGTCTG
TCCTTCCAGGACAGCTACGCCTATCTGGACGACTGGGTGCTGGGCGCCCTCGGCGATCTG
GGCATCCGGGCCTGGTACCAGCCGCTGAACGACATCGCCACCGACCAGGGCAAGATCGCG
GGCGCGGCGCAGAAGCGCGTGGTCGGGCCCGGCGGGGGTCCGGGTGCCGTGCTGCACCAC
GTGACGATGTCGTACGACATCGACGCCGACAAGATGGTCGAGGTGCTGCGGATCGGGCGG
GAGAAGCTGTCCGACAAGGGCACGAAGAGCGCGAAGAAGCGGGTCGATCCGTTGCGCCGG
CAGACGGGGCTGCCGCGCGAGGCCGTCATCGACCGGATGATCGAGTCCTTCCGCGCGCGC
TACGGCCTGGCCGAGGGCAAGGTCACCGACGAGGAGCTGGCACGGGCGGAGGAGCTGGCC
CGCACGAAGTTCGGCTCGACGGAGTGGACGGCGCGCGTGCCGTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR004143 Biotin/lipoate A/B protein ligase (Domain) PF03099 BPL_LplA_LipB
SignalP	No significant hit
TMHMM	No significant hit

Nogl_00430 last last