Ovied_00030 Ovied_00030   Ovied_00050 Ovied_00050

Ovied_00040 : CDS information

close this sectionLocation

Organism
StrainATCC 11891
Entry nameOviedomycin
Contig
Start / Stop / Direction2,389 / 3,627 / + [in whole cluster]
2,389 / 3,627 / + [in contig]
Location2389..3627 [in whole cluster]
2389..3627 [in contig]
TypeCDS
Length1,239 bp (412 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.3 transport
Productputative major facilitator superfamily transporter
Product (GenBank)putative transmembrane efflux protein from the major facilitator superfamily
Gene
Gene (GenBank)ovmE
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[15368568] Genetic organization of the biosynthetic gene cluster for the antitumor angucycline oviedomycin in Streptomyces antibioticus ATCC 11891. (Chembiochem. , 2004)
comment
oviedomycin生合成gene clusterの報告。

OvmE: permease of the major facilitator superfamily

ovmEについては配列解析のみ。
oviedomycinを産生株から排出し、自己抵抗性を与えうる。
Related Reference
ACC
Q0GQS6
PmId
comment
Blast 128th, id32%, 1e-41
Bacillus amyloliquefaciens_pbuE
Purine efflux pump pbuE

we obtained direct evidence for the involvement of PbuE in efflux of not only purine bases, but also purine ribonucleosides.
ACC
P31141
PmId
[1779766] ( , )
comment
Streptomyces lividans_cmlR
Chloramphenicol resistance protein
Blast id35.51%, 3e-22, score100

遺伝子的に不安定なStreptomyces lividans 1326のchloramphenicol resistance geneがクローニングされ、特徴づけされた。この遺伝子、そして隣接するDNA領域は自発的に失われたり、変異体の中で増幅したりする。chloramphenicolはacetyltransferaseや他の酵素によって修飾されず（chloramphenicolはacetylationによって不活化されるので今は不活化されないことを示す）、しかも抵抗株のリボソームはchloramphenicolに対して感受性がある。resistance geneは12TMSsのalpha-helicesを持つと推測される疎水性タンパクをコードし、tetracycline efflux membrane proteinに似ている。

close this sectionSequence

selected fasta
>putative major facilitator superfamily transporter [putative transmembrane efflux protein from the major facilitator superfamily]
MTPPAAQQGRAWRTHACVLALGTFAVGTDAFVIAGLLPDISDSLDISVGAAGQLVSVFSL
AYALLSPVLAAFTARWSRRTVLITGLGVFAVGNVVTALAPDYALVLAARVIAAAGAAMFT
PNAGATAATLAGPEHRGRAIAIVTVGLTASLALGAPLGTVIGEALGWQATMWFVTALALV
VAPVIALRLPDVTLGGPTQLRRRLAPLADRKVAGVLAGTLVAFIGIYLPYTYISAVFEPA
TGGDGDKVAVLLLVFGLAGTAGNLTAGRLADRKGPRTVVIGATLLLTVVFAVMAGVRSSY
PAAVALTAVCGVASWSVTAPQQQRVMALASPGSEPLAVSLNAAVMYLAISLSSALGAGIL
GATGAVWIAWAGAAFAVAAALVTAAAARTERAGAGSAEPEREPAGAQEASAG
selected fasta
>putative major facilitator superfamily transporter [putative transmembrane efflux protein from the major facilitator superfamily]
ATGACCCCTCCCGCCGCACAGCAGGGGCGCGCCTGGCGGACCCACGCCTGTGTGCTCGCA
CTCGGCACGTTCGCCGTCGGCACCGACGCCTTCGTCATCGCCGGCCTGCTCCCCGACATC
AGTGACTCGCTCGACATCTCCGTGGGCGCGGCGGGCCAGCTCGTCAGCGTCTTCTCGCTG
GCCTACGCCCTGCTCTCCCCGGTGCTCGCCGCGTTCACCGCCCGCTGGTCACGCCGTACG
GTGCTGATCACGGGGCTCGGCGTGTTCGCCGTCGGCAACGTGGTCACCGCCCTCGCCCCC
GACTACGCGCTCGTCCTGGCGGCCCGGGTGATCGCCGCGGCCGGCGCCGCGATGTTCACC
CCCAACGCGGGCGCGACCGCGGCGACCCTGGCCGGACCCGAGCACCGGGGCCGGGCGATC
GCCATCGTCACCGTGGGGCTGACCGCCTCGCTCGCGCTGGGCGCGCCGCTCGGCACCGTC
ATCGGCGAGGCGCTGGGCTGGCAGGCCACCATGTGGTTCGTCACCGCGCTCGCCCTGGTC
GTGGCCCCGGTCATCGCCCTGCGGCTGCCCGACGTCACCCTGGGCGGCCCGACCCAGCTG
CGGCGGCGCCTCGCCCCGCTCGCCGACCGCAAGGTCGCCGGGGTACTGGCCGGCACGCTG
GTCGCGTTCATCGGCATCTACCTGCCCTACACCTACATCAGCGCCGTCTTCGAGCCCGCC
ACCGGCGGGGACGGTGACAAGGTGGCCGTACTGCTGCTGGTCTTCGGCCTGGCGGGGACG
GCCGGCAACCTCACCGCGGGCCGGCTCGCCGACCGCAAGGGGCCGCGCACGGTCGTCATC
GGCGCGACGCTGCTGCTGACGGTGGTGTTCGCGGTCATGGCCGGCGTCCGCTCCTCCTAC
CCGGCCGCCGTCGCGCTGACGGCGGTGTGCGGCGTCGCCTCCTGGTCGGTGACCGCGCCG
CAGCAGCAGCGCGTCATGGCGCTGGCCAGCCCCGGCAGCGAGCCCCTGGCCGTCTCCCTC
AACGCCGCCGTGATGTACCTGGCGATCTCGCTGTCCAGCGCGCTGGGCGCCGGGATCCTC
GGTGCGACGGGTGCCGTGTGGATCGCCTGGGCGGGCGCCGCCTTCGCCGTGGCCGCGGCG
CTGGTCACGGCCGCCGCCGCCCGCACCGAGCGGGCCGGCGCCGGGTCCGCGGAGCCGGAG
CGGGAGCCGGCCGGGGCGCAGGAGGCGTCCGCCGGTTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR011701 Major facilitator superfamily (Family)
[19-235] 7.59999999999998e-34 PF07690
PF07690   MFS_1
IPR016196 Major facilitator superfamily domain, general substrate transporter (Domain)
[1-404] 1e-67 SSF103473
SSF103473   MFS_gen_substrate_transporter
IPR020846 Major facilitator superfamily domain (Domain)
[15-391] PS50850
PS50850   MFS
SignalP
[1-30] 0.984 Signal
Eukaryota   
[1-25] 0.334 Signal
Bacteria, Gram-negative   
TMHMM
[13-35] Transmembrane (i-o) [50-72] Transmembrane (o-i) [81-100] Transmembrane (i-o) [110-132] Transmembrane (o-i) [139-161] Transmembrane (i-o) [171-190] Transmembrane (o-i) [211-233] Transmembrane (i-o) [248-270] Transmembrane (o-i) [277-296] Transmembrane (i-o) [300-317] Transmembrane (o-i) [338-360] Transmembrane (i-o) [365-387] Transmembrane (o-i)
Transmembrane 12   
Ovied_00030 Ovied_00030   Ovied_00050 Ovied_00050
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