Ovied_00210 Ovied_00210   Ovied_00230 Ovied_00230

Ovied_00220 : CDS information

close this sectionLocation

Organism
StrainATCC 11891
Entry nameOviedomycin
Contig
Start / Stop / Direction22,849 / 23,652 / + [in whole cluster]
22,849 / 23,652 / + [in contig]
Location22849..23652 [in whole cluster]
22849..23652 [in contig]
TypeCDS
Length804 bp (267 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative 3-oxoacyl-[acyl-carrier-protein] reductase
putative beta-ketoacyl-acyl carrier protein reductase
Product (GenBank)putative 3-keto-acyl carrier protein reductase
GenefabG
Gene (GenBank)
EC number1.1.1.100
Keyword
Note
  • It is supposed that this ORF is not involved in oviedomycin biosynthesis.
Note (GenBank)
  • ORF5; FabG homologue
Reference
ACC
PmId
[15368568] Genetic organization of the biosynthetic gene cluster for the antitumor angucycline oviedomycin in Streptomyces antibioticus ATCC 11891. (Chembiochem. , 2004)
comment
oviedomycin生合成gene clusterの報告。

ORF5: beta-ketoacyl-ACP reductase, lipid metabolism

このORFは、おそらくoviedomycin生合成に関連しないと述べられている。
Related Reference
ACC
A5HIF6
PmId
comment
Blast 49th, id36%, 2e-28
Pseudomonas putida_fabG
3-oxoacyl-acyl-carrier-protein reductase

Pseudomonas putida KCTC1639から、(R)-specific 2-enoyl-CoA hydratase_PhaJ と、3-ketoacyl-acyl carrier protein reductase_FabG をクローニングし、medium-chain-length polyhydroxyalkanoate (mcl-PHA)のための(R)-3-hydroxyalkanoate monomer unitsを過剰補給するために親株で増幅した。

phaJの過剰発現はmcl-PHAを増やすが、fabGの過剰発現では逆に抑制する傾向にあった。これはおそらく、
(R)-3-hydroxyalkanoate monomer units → 3-ketoacyl-CoA
への可逆反応のせいである。
ACC
O54438
PmId
comment
Blast 250th, id35%, 7e-26
Pseudomonas aeruginosa_fabG
3-oxoacyl-[acyl-carrier-protein] reductase

P.aeruginosaのFatty Acid Biosynthetic Gene ClusterをCharacterization.

NADPH binding signature AX2GXGX2AX6G near the NH2 terminus,
fabG knockout mutation単離できない。
in vitro Fab synthesis systemで脂肪酸鎖長延長に精製FabG必須。

close this sectionSequence

selected fasta
>putative 3-oxoacyl-[acyl-carrier-protein] reductase [putative 3-keto-acyl carrier protein reductase]
MTRSVSASGPLQGRIALVTGGSRGIGAATATTLAGLGADVVLTYRSSLDEAKAVAAACEE
KGVKATVLRADLDAADGAEELVTAVRERVDHLDVVVSNACASYPRVPLAGMDPTALGDKV
RNDVVLLHRLTTAFVPAMRERGYGRLVVISSGSSWGPTAPGLAAHGVTKAALEGYVRYAA
DEFGAGGVTVNGLALGFVLTDASSVVPQPARDALANATPAGRLGTPQDIADAVSLLARPE
SEWINGATLPVTGGLNYPLNLSRVRGH
selected fasta
>putative 3-oxoacyl-[acyl-carrier-protein] reductase [putative 3-keto-acyl carrier protein reductase]
ATGACTCGTAGCGTCTCCGCATCCGGCCCGCTTCAGGGCAGGATCGCGCTGGTGACAGGC
GGTAGCCGGGGGATCGGGGCCGCGACGGCCACCACCCTCGCCGGGCTCGGGGCCGACGTC
GTGCTCACATACCGAAGCTCGCTGGACGAGGCCAAGGCCGTGGCGGCCGCGTGCGAGGAA
AAAGGTGTAAAGGCCACGGTGCTCAGGGCCGACCTGGACGCGGCCGACGGCGCCGAGGAA
CTGGTCACCGCCGTGCGGGAACGGGTGGACCACCTCGACGTCGTCGTCAGCAACGCCTGC
GCCTCGTACCCGCGCGTGCCGCTGGCCGGCATGGACCCGACCGCGCTCGGCGACAAGGTC
CGCAACGACGTCGTCCTGCTGCACCGGCTCACCACCGCCTTCGTGCCCGCGATGCGGGAG
CGGGGGTACGGCCGGCTCGTCGTCATCTCCAGCGGCTCGTCCTGGGGCCCGACCGCGCCC
GGCCTCGCCGCGCACGGAGTGACGAAGGCCGCGCTGGAGGGCTACGTCCGGTACGCGGCC
GACGAGTTCGGCGCCGGTGGCGTGACCGTCAACGGCCTCGCCCTGGGCTTCGTCCTCACC
GACGCCTCCTCGGTGGTCCCGCAGCCGGCGCGCGACGCGCTGGCCAACGCCACGCCGGCC
GGGCGGCTCGGCACCCCGCAGGACATCGCCGACGCCGTGTCCCTGCTGGCGCGCCCCGAG
TCGGAGTGGATCAACGGGGCGACCCTCCCCGTGACCGGCGGCCTCAACTACCCGCTGAAC
CTGTCCCGCGTCCGCGGCCACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002347 Glucose/ribitol dehydrogenase (Family)
[15-32] 1.10000150671642e-28 PR00081 [90-101] 1.10000150671642e-28 PR00081 [138-154] 1.10000150671642e-28 PR00081 [165-184] 1.10000150671642e-28 PR00081 [186-203] 1.10000150671642e-28 PR00081 [219-239] 1.10000150671642e-28 PR00081
PR00081   GDHRDH
IPR016040 NAD(P)-binding domain (Domain)
[11-256] 9.3000000000001e-66 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP
[1-38] 0.563 Signal
Eukaryota   
TMHMM No significant hit
Ovied_00210 Ovied_00210   Ovied_00230 Ovied_00230
Page top