Oxzol_00170 Oxzol_00170   Oxzol_00190 Oxzol_00190

Oxzol_00180 : CDS information

close this sectionLocation

Organism
StrainJA3453
Entry nameOxazolomycin
Contig
Start / Stop / Direction62,176 / 65,349 / + [in whole cluster]
62,176 / 65,349 / + [in contig]
Location62176..65349 [in whole cluster]
62176..65349 [in contig]
TypeCDS
Length3,174 bp (1,057 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.4 Other
Productputative trans-acting acyltransferase
Product (GenBank)malonyl CoA-ACP transferase
Gene
Gene (GenBank)ozmM
EC number
Keyword
  • trans AT
  • malonyl-CoA
Note
  • The N-terminal position was modified from original INSDC entry.
Note (GenBank)
Reference
ACC
PmId
[20406823] Oxazolomycin biosynthesis in Streptomyces albus JA3453 featuring an "acyltransferase-less" type I polyketide synthase that incorporates two distinct extender units. (J Biol Chem. , 2010)
comment
※論文中ではLoading moduleをmodule 1としているが、本DBではLoading moduleはmodule 0とするため論文のmodule No.とずれが生じる。


oxazolomycin生合成遺伝子クラスターの報告。

invivo site-directed mutagenesisにより活性サイトのアミノ酸残基をOzmM-AT1の活性サイト Ser81->Glyに、OzmM-AT2の活性サイト Ser402->Glyに変異させた株を作製。
OzmM-AT1変異株はoxazolomycin産生に変化がみられなかったことから潜在的なdomainである可能性を示唆し、OzmM-AT2変異株ではoxazolomycin産生が出来なかったことからoxazolomycin生合成に必須である可能性を示唆した。

さらに、OzmMはmethoxymalonyl-CoA extender unitであるmodule10を除くAT-less PKS modulesすべてのmalonyl-CoA extender unitのloadingに関与すると考察。
module10のmethoxymalonyl-CoA extender unitのloadingにはOzmCが関与するとしている。
Related Reference
ACC
Q8GGP5
PmId
[12598647] Type I polyketide synthase requiring a discrete acyltransferase for polyketide biosynthesis. (Proc Natl Acad Sci U S A. , 2003)
comment
Streptomyces atroolivaceus
Acyltransferase/oxidoreductase(LnmG)

Leinamycin 生合成遺伝子クラスターの報告。
acyltransferase lnmGで唯一のAT activity が確認された。lnmGの不活性化株でLeinamycin biosynthesisが出来ないことを見ている。all six PKS modulesに対して特異的にそして効率的にmalonyl-CoA伸長ユニットのlaodingを触媒することも確認している。

close this sectionPKS/NRPS Module

13
malonyl-CoA
malonyl-CoA
AT6..274
AT336..604

close this sectionSequence

selected fasta
>putative trans-acting acyltransferase [malonyl CoA-ACP transferase]
MSRPVVFQFSGQGSQYSGMGSALYETEPVFRRVMDRLDAAASGELGESVLAALYAPGRGR
AEPFDDIAFTHPAIVMVELAAAETLIASGIRPDLLLGASLGEFTASVLAGVLDADACLRI
LVRQAAAVRDAPRGGMLAVLDDVALHARLPLLRERTEIAARNYPGHFVLAGAHEDLVAAE
EELRARKVVCHRVPVPYAFHSRLMDSGEPLFRSAMAGTELRPPRLPVISCATGGQVERVT
VDHLWRATRLPIEYAGTLAALERRGPFQYVDLGPSGTLHNFARNGLPASSRSRSLPLLSP
LGDDTRSLAAVRAAVPATARPAIPATPLSEDGVMKVYGFPGQGSQAKGMGKDLFEEFPEE
TALADSVLGHSIRELCVEDPRRELKLTRFTQPALYVVSALAWLRERRENPVPPDFLVGHS
LGEYVALFAAGSFDFETGLRLVARRGELMSRADGGRMAAVLKCDLETVEGALAGHGLTGL
DIANHNAPGQFVIAGPTEQITAAKPVFEGLGAHYVQLNVSAPFHSRYLRDTAEEFGRYLE
GFTLRDPAVPVIANVDARPYRPGEVARQLARQIASPVRWTDTIRYLMGLGDFEFVELGPG
RVLGKLVAKIRAEAEPLVPAKAPATGATAVQPVAARPGGGPAALGAATFRERYGLRYACL
AGSMYGGVSGAELLVRLAKAGGMGFFGSGGLSPDDVGAGLDAVRAGLGADGAYGANLLHR
HTDPHHEAALVDLFLRKGVRTVEASGFLRITPALVKYRLSGGRVLAKVSSTDMATAFLAP
APPAIVRELVDSGQVSESAAARYADVPLADDLCVETGSGWYEAVGSLATLLPAVLRLRDA
AAGAGPRVHVGAAGGIGCPEAAAAAFLLGADFLLTGSINQCTPEADTSTAVKDLLQSLDV
HDVALAPAAEMFELGVRAQVVKRGVFLPARAGKLHDLWRRHDSLDAVDAPTRRQIEEKFL
RAALPTAAASGGDPKRHMADAFRSYLDRGFDLARRGEPGRTVDYLVYCGPAMGAFNSWAR
GTVLESWQSRHVDAITEELMTGTAALLRERAAAFAAL
selected fasta
>putative trans-acting acyltransferase [malonyl CoA-ACP transferase]
TTGAGCCGCCCGGTCGTCTTCCAGTTCTCCGGACAGGGCTCGCAGTACAGCGGGATGGGA
TCCGCCCTGTACGAGACGGAACCGGTCTTCCGCCGCGTCATGGACCGCCTCGACGCGGCG
GCGTCCGGCGAACTCGGCGAGTCCGTCCTCGCGGCGCTCTACGCGCCGGGCCGCGGCCGA
GCCGAACCGTTCGACGACATCGCGTTCACCCACCCGGCGATCGTCATGGTCGAACTCGCC
GCCGCGGAGACCCTCATCGCCTCCGGCATCCGGCCCGACCTGCTGCTCGGCGCCAGCCTG
GGGGAGTTCACCGCGTCCGTGCTCGCGGGCGTCCTCGACGCCGACGCGTGTCTGCGGATC
CTGGTCCGCCAGGCCGCCGCGGTGCGTGACGCCCCGCGCGGCGGCATGCTCGCCGTCCTC
GACGACGTGGCGCTCCACGCGCGGCTCCCGCTGCTGCGCGAGCGCACCGAGATCGCCGCC
CGCAACTATCCGGGACACTTCGTCCTCGCGGGCGCCCACGAGGACCTGGTGGCCGCCGAG
GAGGAACTGCGGGCCCGCAAGGTGGTGTGCCACCGCGTCCCCGTCCCGTACGCCTTCCAC
TCGCGCCTGATGGACTCCGGCGAGCCGCTGTTCCGCTCCGCCATGGCAGGCACCGAGCTG
CGACCGCCGCGGCTCCCCGTGATCTCCTGCGCGACCGGCGGCCAGGTCGAGCGTGTCACG
GTCGACCACCTGTGGCGGGCGACCCGGCTGCCCATCGAGTACGCCGGCACGCTGGCCGCC
CTCGAACGCCGCGGCCCCTTCCAGTACGTGGACCTCGGCCCCTCCGGCACCCTGCACAAC
TTCGCCCGCAACGGCCTGCCCGCGTCATCGCGCTCCCGGTCGCTGCCGCTGCTGAGCCCA
CTGGGTGACGACACCCGCTCCCTGGCCGCCGTGCGCGCCGCCGTACCGGCCACCGCGCGC
CCCGCGATCCCCGCAACCCCCCTATCCGAGGACGGCGTCATGAAGGTGTACGGATTCCCC
GGCCAGGGCAGCCAGGCCAAGGGCATGGGCAAGGACCTCTTCGAGGAGTTCCCCGAGGAG
ACCGCGCTCGCCGACTCGGTGCTCGGCCACTCGATCCGCGAGCTGTGCGTCGAGGACCCC
CGCCGCGAGCTGAAGCTCACGCGGTTCACCCAGCCCGCCCTCTACGTCGTCAGCGCCCTC
GCCTGGCTGCGCGAGCGGCGCGAGAACCCCGTACCGCCCGACTTCCTCGTCGGCCACAGC
CTCGGCGAGTACGTGGCGCTGTTCGCCGCCGGGTCCTTCGACTTCGAGACCGGCCTCCGC
CTGGTCGCCCGCCGCGGCGAGCTGATGAGCCGCGCCGACGGCGGGCGGATGGCGGCGGTC
CTCAAGTGCGACCTCGAAACCGTTGAGGGAGCCCTGGCCGGACACGGCCTGACCGGTCTG
GACATCGCGAACCACAACGCGCCCGGCCAGTTCGTGATCGCCGGGCCCACCGAGCAGATC
ACCGCCGCCAAGCCGGTCTTCGAGGGCCTCGGCGCGCACTACGTGCAGCTCAACGTCAGC
GCCCCCTTCCACTCCCGTTATCTGCGGGACACCGCGGAGGAGTTCGGCCGCTACCTGGAG
GGGTTCACCCTGCGCGACCCCGCCGTCCCGGTCATCGCCAACGTCGACGCCCGGCCCTAC
CGGCCCGGCGAGGTGGCGCGGCAGCTCGCCCGCCAGATCGCCTCCCCCGTCCGGTGGACC
GACACGATCCGCTACCTCATGGGCCTGGGCGACTTCGAGTTCGTGGAATTGGGGCCGGGA
CGGGTGCTCGGCAAGCTCGTCGCGAAGATCAGGGCGGAGGCCGAGCCGCTCGTACCGGCG
AAGGCGCCCGCGACCGGGGCCACTGCCGTGCAGCCGGTGGCCGCGCGGCCCGGCGGTGGT
CCCGCCGCCCTCGGGGCCGCCACGTTCCGCGAACGCTACGGCCTGCGGTACGCCTGCCTC
GCGGGCTCCATGTACGGCGGTGTGTCCGGTGCGGAACTCCTCGTGCGACTCGCGAAGGCG
GGCGGGATGGGCTTCTTCGGCAGCGGCGGGCTCTCCCCGGACGACGTCGGGGCGGGGCTC
GACGCGGTGCGCGCCGGACTCGGGGCCGACGGAGCCTACGGCGCCAACCTGCTGCACCGG
CACACCGACCCGCACCACGAGGCGGCGCTGGTCGACCTCTTCCTGCGCAAGGGCGTCCGC
ACCGTCGAGGCCTCCGGCTTCCTGCGGATCACCCCGGCCCTGGTGAAGTACCGGCTCAGC
GGCGGCCGGGTGCTCGCCAAGGTGTCGAGCACCGACATGGCCACCGCCTTCCTCGCGCCC
GCGCCGCCCGCGATCGTCCGGGAACTGGTGGACTCGGGGCAGGTCTCCGAGTCGGCCGCC
GCCCGGTACGCGGACGTGCCACTGGCCGACGACCTCTGCGTGGAGACCGGCTCCGGCTGG
TACGAGGCCGTCGGCAGCCTCGCCACCCTGCTGCCCGCCGTCCTCCGCCTGCGCGACGCC
GCGGCCGGTGCGGGGCCGCGGGTGCACGTGGGCGCCGCGGGAGGCATCGGCTGCCCGGAG
GCGGCCGCCGCCGCGTTCCTGCTCGGCGCGGACTTCCTGCTCACCGGATCCATCAACCAG
TGCACGCCCGAGGCGGACACCAGCACGGCCGTCAAGGACCTGCTCCAGTCGCTCGACGTC
CACGACGTGGCCCTCGCGCCCGCCGCCGAGATGTTCGAGCTCGGCGTACGCGCGCAGGTC
GTCAAGCGCGGCGTGTTCCTGCCGGCGCGCGCCGGAAAGCTGCACGACCTGTGGCGGCGC
CACGACTCCCTCGACGCCGTCGACGCGCCGACCCGCCGCCAGATCGAGGAGAAGTTCCTG
CGCGCGGCGCTGCCCACGGCAGCCGCCTCCGGCGGTGACCCCAAGCGGCACATGGCCGAC
GCCTTCCGGAGCTACCTCGATCGCGGCTTCGACCTCGCGCGGCGCGGTGAACCCGGCAGG
ACCGTCGACTACCTCGTCTACTGCGGGCCCGCCATGGGCGCCTTCAACTCCTGGGCGCGC
GGCACCGTCCTGGAGTCCTGGCAGTCGCGCCACGTCGACGCGATCACCGAAGAGCTGATG
ACCGGGACGGCGGCGCTGCTGCGCGAACGCGCCGCCGCGTTCGCCGCGCTCTGA
[13] AT6..274
[13] malonyl-CoA197..201
[13] AT336..604
[13] malonyl-CoA521..525
[13] AT16..822
[13] malonyl-CoA589..603
[13] AT1006..1812
[13] malonyl-CoA1561..1575

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001227 Acyl transferase domain (Domain)
[196-299] 5.3e-49 G3DSA:3.40.366.10 [519-613] 5.6e-54 G3DSA:3.40.366.10
G3DSA:3.40.366.10   no description
IPR004136 2-nitropropane dioxygenase, NPD (Domain)
[657-904] 6.9e-12 PF03060
PF03060   NMO
IPR004410 Malonyl CoA-acyl carrier protein transacylase, FabD-type (Family)
[336-612] 1e-88 TIGR00128
TIGR00128   fabD: malonyl CoA-acyl carrier protein transacylas
IPR013785 Aldolase-type TIM barrel (Domain)
[649-906] 4.1e-21 G3DSA:3.20.20.70
G3DSA:3.20.20.70   no description
IPR014043 Acyl transferase (Domain)
[6-274] 6.7e-41 PF00698 [336-604] 5e-29 PF00698
PF00698   Acyl_transf_1
IPR014179 PfaD family protein (Family)
[642-1050] 3.8e-140 TIGR02814
TIGR02814   pfaD_fam: PfaD family protein
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
[4-305] 6e-45 SSF52151 [333-636] 4.1e-57 SSF52151
SSF52151   FabD/lysophospholipase-like
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
[132-196] 6.3e-11 SSF55048 [453-520] 6.4e-17 SSF55048
SSF55048   Probable ACP-binding domain of malonyl-CoA ACP transacylase
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
[9-322] 8.6e-38 SM00827 [339-638] 3.4e-29 SM00827
SM00827   Acyl transferase domain in polyketide syntha
SignalP No significant hit
TMHMM No significant hit
Oxzol_00170 Oxzol_00170   Oxzol_00190 Oxzol_00190
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