Pact_00170 : CDS information

Location

Organism	Streptomyces pactum
Strain	ATCC 27456 (=NBRC 13433)
Entry name	Pactamycin
Contig	AB303063
Start / Stop / Direction	22,548 / 23,603 / + [in whole cluster] 22,548 / 23,603 / + [in contig]
Location	22548..23603 [in whole cluster] 22548..23603 [in contig]
Type	CDS
Length	1,056 bp (351 aa)

Annotation

Category	3.3 modification reduction
Product	putative oxidoreductase
Product (GenBank)	putative NAD dependent dehydrogenase
Gene	pctP ptmN
Gene (GenBank)	pctP
EC number
Keyword
Note
Note (GenBank)
Reference	ACC A8R0J9 PmId [17827660] Cloning of the pactamycin biosynthetic gene cluster and characterization of a crucial glycosyltransferase prior to a unique cyclopentane ring formation. (J Antibiot (Tokyo). , 2007) [19670201] Deciphering pactamycin biosynthesis and engineered production of new pactamycin analogues. (Chembiochem. , 2009) comment [PMID:17827660] Pactamycin生合成遺伝子クラスターの報告。 PctP: NAD dependent dehydrogenase 相同性より機能付けされている。このORFの機能解析はされていない。シクロペンタン環の修飾過程において、cyclopentane中間体はputative　dehydrogenase PctE or PctPによってoxidation and cyclizationが起こりエネジオール中間体を経由してそのisomerへ異性化されると推測している。 [PMID:19670201] S.pactum ATCC27456のPactamycin生合成遺伝子クラスターの報告。 PtmN: oxidoreductase PtmNの機能解析はされていない。
Related Reference	ACC Q2I8V6 PmId [16461673] Catabolism of 1,5-anhydro-D-fructose in Sinorhizobium morelense S-30.7.5: discovery, characterization, and overexpression of a new 1,5-anhydro-D-fructose reductase and its application in sugar analysis and rare sugar synthesis. (Appl Environ Microbiol. , 2006) comment Ensifer adhaerens (Sinorhizobium morelense)_afr 1,5-anhydro-D-fructose reductase(EC 1.1.1.292) UniProt entry name変更有り：Q2I8V6_9RHIZ→AFR_ENSAD --- Sinorhizobium morelense(UniProtでは E.adhaerens) S-30.7.5株由来の1,5-anhydro-D-fructose(AF) reductaseの単離およびcharacterization. nativeだけでなく、His(6)-tagged recombinant(E.coliにて発現)も作製。 AFから1,5-anhydro-D-mannitol(AM)への触媒活性を確認。 -->NADPH-dependent monomeric reductase
Family/Domain	FD IPR000683, IPR004104 comment [IPR000683] Oxidoreductase, N-terminal (Domain) [IPR004104] Oxidoreductase, C-terminal (Domain)

Sequence

>putative oxidoreductase [putative NAD dependent dehydrogenase]
MTREKPIRFAAVGAGRVFQRYHLPCVDARDDVELVGLVDADADRAASVAAGRPGVWTGTD
VARLIREARPDALSVCTPNDAHAAPVLAALDAGIPVLCEKPLAATVDEARRMAEHPAAAE
LLAVNMPFRCHSLTAPFAEAAGKGAQRVEVSFVTPGNRVWRACTPWYGDARRAGGGALLD
LGPHAIDLLMTVFGHPDVEACTVNAEGVEEQAELQLSFQGLPATIRIDRAARRMETAVTV
TTADGAHVLDLRRNELRLADGTVRQGADRPELAAISAFFDAVTGAATGAAGAAGDGPAAG
GAAGTSGADAAGAGATGVTGAGAVGAREALAVQLVVDEAYRRARGAAPAVT

>putative oxidoreductase [putative NAD dependent dehydrogenase]
ATGACCCGCGAGAAGCCGATCCGGTTCGCCGCGGTGGGGGCCGGGCGGGTGTTCCAGCGC
TACCACCTGCCCTGCGTCGACGCCCGGGACGACGTGGAACTGGTGGGGCTGGTGGACGCC
GACGCGGACCGGGCGGCGTCCGTCGCGGCCGGCCGGCCGGGGGTGTGGACCGGCACCGAC
GTCGCGCGGCTGATCCGCGAGGCCCGGCCGGACGCGCTCAGCGTCTGCACCCCCAACGAC
GCCCACGCCGCGCCGGTGCTGGCCGCGCTGGACGCCGGTATCCCGGTGCTCTGCGAGAAA
CCCCTGGCCGCCACGGTGGACGAGGCGCGGCGGATGGCCGAGCACCCGGCCGCGGCGGAG
CTGCTGGCGGTGAACATGCCGTTCCGCTGCCACTCGCTGACCGCGCCGTTCGCCGAGGCG
GCCGGCAAGGGCGCGCAGCGGGTGGAGGTCTCCTTCGTCACCCCCGGCAACCGGGTGTGG
CGGGCCTGCACCCCCTGGTACGGCGACGCCCGGCGGGCCGGCGGCGGCGCCCTGCTGGAC
CTCGGTCCGCACGCCATCGACCTGCTGATGACCGTCTTCGGCCATCCGGACGTCGAGGCG
TGCACGGTGAACGCCGAGGGGGTGGAGGAACAGGCCGAGCTCCAACTGTCCTTCCAGGGC
CTGCCGGCCACGATCCGGATCGACCGGGCCGCCCGCCGGATGGAGACCGCGGTGACCGTC
ACCACGGCCGACGGCGCGCACGTGCTGGACCTGCGGCGCAACGAGCTGCGGCTCGCCGAC
GGCACCGTCCGGCAGGGCGCCGACCGCCCGGAACTCGCCGCGATCTCCGCGTTCTTCGAC
GCGGTGACCGGCGCGGCGACCGGCGCGGCGGGGGCCGCTGGGGACGGCCCGGCGGCTGGC
GGTGCGGCCGGCACGTCCGGAGCGGATGCGGCCGGTGCGGGCGCGACCGGTGTGACCGGG
GCGGGGGCGGTGGGCGCCCGGGAGGCGCTCGCGGTCCAGCTCGTCGTGGACGAGGCCTAC
CGCCGCGCCCGGGGCGCGGCCCCGGCGGTGACCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000683 Oxidoreductase, N-terminal (Domain) PF01408 GFO_IDH_MocA IPR004104 Oxidoreductase, C-terminal (Domain) PF02894 GFO_IDH_MocA_C IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd
SignalP	Eukaryota
TMHMM	No significant hit

Pact_00160 Pact_00180