Polk_00010 : CDS information

Location

Organism	Streptomyces diastatochromogenes
Strain	Tü6028
Entry name	Polyketomycin
Contig	FJ483966
Start / Stop / Direction	2 / 358 / + [in whole cluster] 2 / 358 / + [in contig]
Location	2..358 [in whole cluster] 2..358 [in contig]
Type	CDS
Length	357 bp (118 aa)

Annotation

Category	5.1 general function
Product	putative S-adenosylmethionine synthetase(fragment) putative methionine adenosyltransferase(fragment)
Product (GenBank)	PokSAMS
Gene	pokSAMS metK
Gene (GenBank)	pokSAMS
EC number	2.5.1.6
Keyword	SAM
Note
Note (GenBank)	putative S-adenosylmethionine synthetase
Reference	ACC C0JW96 PmId [19266534] Organisation of the biosynthetic gene cluster and tailoring enzymes in the biosynthesis of the tetracyclic quinone glycoside antibiotic polyketomycin. (Chembiochem. , 2009) comment polyketomycin生合成clusterの解析論文。機能推定は配列解析から。 pokSAMS: S-adenosylmethionine synthetase incomplete ORF
Related Reference	ACC Q938W7 PmId [11594346] Influence of ancillary genes, encoding aspects of methionine metabolism, on tylosin biosynthesis in Streptomyces fradiae. (J Antibiot (Tokyo). , 2001) comment Blast 21st, id81%, 1e-49 Streptomyces fradiae_metK S-adenosylmethionine synthase(EC 2.5.1.6) tyl clusterに含まれる補助的遺伝子・metF(N5,N10-methylenetetrahydrofolate reductase) ・orf9(unknown) ・metK(S-adenosylmethionine synthase) を削除するとtylosin産生減少。これら補助遺伝子は二次代謝の際にtransmethylation能力を拡大する。
Family/Domain	FD IPR022630 comment [IPR022630] S-adenosylmethionine synthetase, C-terminal (Domain) function: methionine adenosyltransferase activity

Sequence

>putative S-adenosylmethionine synthetase(fragment) [PokSAMS]
PSKVDRSAAYAMRWVTKNVVAASLARRWEVQVAYAIGKADPVGLLVETFGTATVDPEKIE
NAVSAVFDLRPAALIRDLDLVRPIYAQTAAYGHFGREMPDFTWERTDRVDALRRAAGL

>putative S-adenosylmethionine synthetase(fragment) [PokSAMS]
CCCTCCAAGGTGGACCGTTCGGCGGCCTACGCCATGCGGTGGGTTACCAAGAACGTCGTC
GCCGCCTCGCTCGCGCGCCGCTGGGAGGTCCAGGTCGCCTACGCGATCGGCAAGGCCGAT
CCCGTCGGGCTCTTGGTGGAGACCTTCGGCACCGCGACCGTCGACCCGGAGAAGATCGAG
AACGCCGTCTCGGCGGTCTTCGACCTCCGGCCCGCCGCCCTCATCCGCGACCTCGACCTG
GTGCGGCCGATCTACGCCCAGACCGCGGCCTACGGCCACTTCGGGCGAGAGATGCCGGAC
TTCACCTGGGAGCGCACCGACCGCGTCGACGCGCTGCGCCGGGCCGCCGGTCTCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR022630 S-adenosylmethionine synthetase, C-terminal (Domain) PF02773 S-AdoMet_synt_C IPR022636 S-adenosylmethionine synthetase superfamily (Domain) SSF55973 S-adenosylmethionine synthetase
SignalP	Bacteria, Gram-positive
TMHMM	No significant hit

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