Polk_00020 : CDS information

Location

Organism	Streptomyces diastatochromogenes
Strain	Tü6028
Entry name	Polyketomycin
Contig	FJ483966
Start / Stop / Direction	395 / 1,375 / + [in whole cluster] 395 / 1,375 / + [in contig]
Location	395..1375 [in whole cluster] 395..1375 [in contig]
Type	CDS
Length	981 bp (326 aa)

Annotation

Category	5.1 general function
Product	putative phosphotransferase
Product (GenBank)	PokR3
Gene
Gene (GenBank)	pokR3
EC number
Keyword
Note
Note (GenBank)	putative kinase
Reference	ACC C0JW97 PmId [19266534] Organisation of the biosynthetic gene cluster and tailoring enzymes in the biosynthesis of the tetracyclic quinone glycoside antibiotic polyketomycin. (Chembiochem. , 2009) comment polyketomycin生合成clusterの解析論文。機能推定は配列解析から。 pokR3: kinase
Family/Domain	FD IPR011611, IPR002173 comment [IPR011611] Carbohydrate/purine kinase (Domain) [IPR002173] Carbohydrate/puine kinase, PfkB, conserved site (Conserved_site)

Sequence

>putative phosphotransferase [PokR3]
MRIAVTGSIATDHLMAFPGRFVDQLMSEHLEKVSLSFLADTLEIRRGGVAANIAVGLGRL
GLTPVLVGAAGTDFAEYRDWLRANGVDTGSVRVSATRHTARFVCTTDADQNQIATFYAGA
MVEAREIALAGVVERVGGVGLVMVGPDDPEAMLRHTDACRTLGLDFAADPSQQLARLGRE
ETRRLVDGARFLFTNAYEAALLQERTGWSRAEVLERVGSWLVTHGPEGVAVLRSGHPELR
LPAVEAAEVVDPTGAGDGFRAGFLAGAARGWPQERAARLGCALATLVLESLGTQEYKLSA
AELHLRTEQAYGAAAAAEITHDLERR

>putative phosphotransferase [PokR3]
GTGCGCATCGCCGTCACCGGATCCATCGCGACGGACCACCTCATGGCCTTTCCCGGGCGC
TTCGTCGACCAGCTCATGAGCGAGCACCTGGAGAAGGTGTCGCTGTCGTTCCTCGCCGAC
ACGCTGGAGATCAGGCGCGGCGGGGTGGCCGCCAACATCGCCGTGGGCCTCGGCCGCCTC
GGGCTCACTCCGGTCCTGGTGGGCGCGGCCGGCACCGACTTCGCCGAGTACCGCGACTGG
CTGCGCGCCAACGGCGTCGACACCGGGTCGGTGCGGGTGAGCGCCACCCGGCACACCGCC
CGCTTCGTGTGCACCACCGACGCCGACCAGAACCAGATCGCCACCTTCTACGCCGGCGCG
ATGGTCGAGGCACGGGAGATCGCGCTGGCCGGCGTCGTCGAACGGGTCGGCGGCGTCGGC
CTGGTGATGGTGGGACCCGACGACCCCGAGGCCATGCTCCGGCACACCGACGCGTGCCGG
ACACTCGGACTGGACTTCGCGGCCGACCCCTCGCAGCAACTCGCCCGACTGGGACGGGAG
GAGACCCGGCGGCTCGTCGACGGGGCCCGCTTCCTGTTCACCAACGCTTACGAGGCGGCC
CTGCTCCAGGAACGAACGGGCTGGAGCAGGGCCGAGGTCCTCGAACGGGTCGGCAGCTGG
CTCGTCACCCACGGCCCCGAGGGCGTCGCCGTGCTCCGCTCCGGACACCCGGAGTTGCGG
CTGCCCGCGGTCGAGGCGGCCGAGGTCGTGGACCCCACCGGCGCCGGCGACGGCTTCCGC
GCCGGATTCCTGGCCGGCGCCGCACGGGGATGGCCGCAGGAACGGGCCGCCCGGCTCGGG
TGCGCCCTGGCGACCCTCGTGCTGGAGTCCCTCGGCACCCAGGAGTACAAGCTGTCCGCC
GCCGAACTGCACCTGAGGACCGAGCAGGCCTACGGGGCCGCCGCTGCCGCCGAGATCACC
CACGACCTGGAGAGGCGATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR002173 Carbohydrate/puine kinase, PfkB, conserved site (Conserved_site) PS00584 PFKB_KINASES_2 PS00583 PFKB_KINASES_1 IPR011611 PfkB (Domain) PF00294 PfkB
SignalP	No significant hit
TMHMM	No significant hit

Polk_00010 Polk_00030