Polk_00020 Polk_00020   Polk_00040 Polk_00040

Polk_00030 : CDS information

close this sectionLocation

Organism
StrainTü6028
Entry namePolyketomycin
Contig
Start / Stop / Direction1,372 / 2,619 / + [in whole cluster]
1,372 / 2,619 / + [in contig]
Location1372..2619 [in whole cluster]
1372..2619 [in contig]
TypeCDS
Length1,248 bp (415 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative NDP-hexose C-3 C-methyltransferase
Product (GenBank)PokS8
Gene
Gene (GenBank)pokS8
EC number2.1.1.-
Keyword
  • alpha-L-axenose
Note
Note (GenBank)
  • putative dNDP-hexose 3-C-methyltransferase
Reference
ACC
PmId
[19266534] Organisation of the biosynthetic gene cluster and tailoring enzymes in the biosynthesis of the tetracyclic quinone glycoside antibiotic polyketomycin. (Chembiochem. , 2009)
comment
polyketomycin生合成clusterの解析論文。

機能推定は配列解析から。
pokS8: dNDP-hexose 3-C-methyltransferase
Related Reference
ACC
Q9XC68
PmId
[11180386] Insights into the Branched-Chain Formation of Mycarose: Methylation Catalyzed by an (S)-Adenosylmethionine-Dependent Methyltransferase We are grateful to Dr. Eugene Seno and the Lilly Research Laboratories for their generous gift of the plasmid pHJL311 and to the National Institutes of Health for grants (GM 35 906 and 54 346). H.-w.L. also thanks the National Institute of General Medical Sciences for a MERIT Award. T.M.H. was a trainee of the National Institute of General Medical Sciences (Biotechnology Training Grant: 2 T32 GM08347). (Angew Chem Int Ed Engl. , 2001)
comment
Blast 2nd, id66%, 1e-152
Streptomyces fradiae_tylCIII
NDP-hexose 3-C-methyltransferase TylCIII

abstract:
TylC3は(S)-adenosylmethylthionine(AdoMet)-dependent enzymeで、L-mycaroseの生合成においてmethyl分枝の付着を触媒する。
C-3 methylationは立体配置の反転を進め、いかなるcofactorsの補助も必要としない。
代謝回転速度は1.4±0.1 min-1

この論文を引用しているEryBIII に関する論文によると、axial立体配置でのC-3 OH基を持つD-sugarを基質とするようだ。
ACC
A4F7P8
PmId
[15610855] Engineering biosynthetic pathways for deoxysugars: branched-chain sugar pathways and derivatives from the antitumor tetracenomycin. (Chem Biol. , 2004)
comment
Blast 5th, id64%, 1e-144
Saccharopolyspora erythraea_eryBIII
EryBIII NDP-4-keto-2,6-dideoxyhexose 3-C-methyltransferase

ery genesを含むplasmidを構築。
その結果から、
EryBIII はL-中間体で働かない。
C-3でequatorial OH基を所有するNDP-2,6-dideoxy-D-sugar基質を変換できない。

よってC-3でaxialなOH基を持つNDP-2,6-dideoxy-D-sugarで働くC-3-methyltransferaseである。
そのためEryBVII による5-epimerizationは、3-C-methylateされた基質に起こる。

close this sectionSequence

selected fasta
>putative NDP-hexose C-3 C-methyltransferase [PokS8]
MTIKECRICGNRTLLPILDLGEQALTGIFPRTRDEVVPSVPLELVRCSPDGCGLVQLRHT
ADFGLMYGEGYGYRSGLGEFMIRHLAGKAALLTDLVRPGPDDLVLDIGSNDSTLLRAYPA
DGPTLVGIDPTGENFREYYPEHVRLVPEFFSREVFTGHFGRRKAKIVTSIAMFYDLPRPL
DFMRDVHDILDDDGVWLLEQELQCPRLIESPPAYDVVVPTKHLEYYALRPDSSGWRARTG
LSVIRAELKLPCTAASLCVVLAKNPARHHVDEAGIAAVRAHEAQLGLDTMAPFEAFAHRA
AEYRDGLRAFLDTSREAGRLTLGYGASTKGNVILQYCGITERDLPCIGEVNKDKDGCFTP
GTGIPVVTESEAKSRRPDQLLVLPWIYRDGFVERERAYRESGGKLVFPLPELSIV
selected fasta
>putative NDP-hexose C-3 C-methyltransferase [PokS8]
ATGACGATCAAAGAGTGCCGCATCTGCGGCAACCGCACCCTGCTGCCGATACTCGACCTG
GGCGAACAGGCCCTCACCGGCATCTTCCCCCGCACCCGCGACGAGGTGGTCCCCTCCGTA
CCCCTCGAACTCGTGCGCTGCTCACCGGACGGCTGCGGACTCGTCCAACTGCGGCACACC
GCCGACTTCGGGCTGATGTACGGCGAGGGCTACGGATACCGGTCCGGACTCGGTGAGTTC
ATGATCCGGCACCTCGCGGGCAAGGCCGCCCTGCTCACCGACCTGGTGCGCCCGGGCCCC
GACGACCTCGTCCTGGACATCGGCAGCAACGACTCCACCCTCCTGCGGGCCTACCCCGCG
GACGGACCCACCCTGGTCGGCATCGACCCGACCGGAGAGAACTTCCGCGAGTACTATCCC
GAACACGTCAGGCTCGTTCCCGAGTTCTTCTCCCGCGAGGTCTTCACCGGGCACTTCGGC
CGGCGCAAGGCCAAGATCGTCACCTCGATCGCGATGTTCTACGACCTGCCGCGCCCCCTC
GACTTCATGCGGGACGTCCACGACATCCTCGACGACGACGGGGTGTGGCTGCTGGAACAA
GAGCTACAATGCCCGCGATTGATCGAAAGCCCACCCGCGTACGACGTTGTTGTGCCCACG
AAGCACCTGGAGTACTACGCGCTGCGGCCAGATTCGAGTGGATGGCGGGCGCGCACCGGA
CTGAGCGTCATCCGGGCGGAGCTTAAACTTCCGTGTACGGCGGCAAGCCTCTGCGTCGTG
CTCGCCAAGAACCCCGCACGCCACCACGTCGACGAGGCCGGGATCGCCGCGGTCCGCGCC
CACGAGGCACAGCTGGGTCTGGACACCATGGCCCCCTTCGAGGCCTTCGCCCACCGGGCG
GCCGAGTACCGCGACGGGCTGCGCGCCTTCCTCGACACCTCCCGCGAGGCCGGCCGGCTG
ACCCTCGGGTACGGCGCGTCGACCAAGGGCAACGTCATCCTCCAGTACTGCGGCATCACC
GAACGCGACCTGCCCTGCATCGGCGAGGTCAACAAGGACAAGGACGGCTGCTTCACCCCC
GGCACCGGCATTCCCGTCGTCACCGAGTCCGAGGCCAAGTCCCGGCGGCCCGACCAGCTG
CTCGTGCTGCCCTGGATCTACCGGGACGGCTTCGTCGAACGGGAGCGTGCGTACCGCGAG
AGCGGCGGCAAGCTGGTCTTCCCGCTACCCGAACTCAGCATCGTGTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR013630 Methyltransferase putative zinc binding domain (Domain)
[6-68] 2.4e-21 PF08421
PF08421   Methyltransf_13
IPR013691 C-methyltransferase (Domain)
[256-410] 4.2e-51 PF08484
PF08484   Methyltransf_14
SignalP No significant hit
TMHMM No significant hit
Polk_00020 Polk_00020   Polk_00040 Polk_00040
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