Polk_00070 : CDS information

Location

Organism	Streptomyces diastatochromogenes
Strain	Tü6028
Entry name	Polyketomycin
Contig	FJ483966
Start / Stop / Direction	8,839 / 7,907 / - [in whole cluster] 8,839 / 7,907 / - [in contig]
Location	complement(7907..8839) [in whole cluster] complement(7907..8839) [in contig]
Type	CDS
Length	933 bp (310 aa)

Annotation

Category	4.3 transport
Product	putative drug resistance ABC transporter ATP-binding protein
Product (GenBank)	PokABC1
Gene
Gene (GenBank)	pokABC1
EC number
Keyword
Note
Note (GenBank)	putative ABC transporter ATP-binding component
Reference	ACC C0JWA2 PmId [19266534] Organisation of the biosynthetic gene cluster and tailoring enzymes in the biosynthesis of the tetracyclic quinone glycoside antibiotic polyketomycin. (Chembiochem. , 2009) comment polyketomycin生合成clusterの解析論文。機能推定は配列解析から。 pokABC1: ABC transporter, ATP-binding component おそらく産生細胞外へのpolyketomycin輸送に関連する。
Related Reference	ACC Q53716 PmId [8326867] Streptomyces antibioticus contains at least three oleandomycin-resistance determinants, one of which shows similarity with proteins of the ABC-transporter superfamily. (Mol Microbiol. , 1993) comment BLAST id53% Streptomyces antibioticus_oleC-ORF4 ATP-binding protein S. antibioticsでOleanomycinに抵抗性を示す遺伝子を単離。 OleC plasmidでクローニングされたORFが5つあり、 Orf4 (NBD), Orf5(TMD)がABC transorterとして働いているのではないかと推論。
Related Reference	ACC P32010 NITE Adria_00130 PmId [1924314] A bacterial analog of the mdr gene of mammalian tumor cells is present in Streptomyces peucetius, the producer of daunorubicin and doxorubicin. (Proc Natl Acad Sci U S A. , 1991) [9006006] Expression and characterization of DrrA and DrrB proteins of Streptomyces peucetius in Escherichia coli: DrrA is an ATP binding protein. (J Bacteriol. , 1997) [15709779] Biochemical characterization of domains in the membrane subunit DrrB that interact with the ABC subunit DrrA: identification of a conserved motif. (Biochemistry. , 2005) [19063901] Translational coupling controls expression and function of the DrrAB drug efflux pump. (J Mol Biol. , 2009) [20158521] Daunorubicin efflux in Streptomyces peucetius modulates biosynthesis by feedback regulation. (FEMS Microbiol Lett. , 2010) [25721665] Characterization of a novel domain 'GATE' in the ABC protein DrrA and its role in drug efflux by the DrrAB complex. (Biochem Biophys Res Commun. , 2015) comment BLAST id52% Streptomyces peucetius_drrA Daunorubicin/doxorubicin resistance ATP-binding protein DrrA -- [PMID: 1924314](1991) DrrAは排出ABC transporterのATP結合タンパク質。Mg2+依存的にATP (or GTP) を結合。 doxorubicin(DXR) or daunorubicin(DNR)の添加で活性が上昇。 DrrBはABC-2 type transporterといわれているもの。 -- さらにいくつかの続報がでており、DrrABはATP依存的なDXRとDNRの排出ポンプとされている(PMID: 15709779)。上記著者らでの最新の続報は[PMID: 19063901](2009) -- 違うグループでも報告あり。上記既報をrefとして採用している。 [PMID: 20158521](2010) drrAB(-)mutantはDNRに高い感受性あり。このmutantはwildと比べて、薬剤産生が10倍減。 key regulator dnrI and polyketide synthase gene dpsAの発現が激減。 regulatory genes dnrO and dnrNの発現は増。これらの結果から細胞内DNR濃度に基づくFeedback regulationを提唱。

Sequence

>putative drug resistance ABC transporter ATP-binding protein [PokABC1]
MTSAIQARGLAKRFGETAALTSVDLDVEAGTVLGLLGPNGAGKTTVVRILATLLRPDAGQ
AFIHGHDVTKSPDRVREIIGLTGQFAAVDDSLTGVENLLFLGRLLGKSRADARGTALSLL
ERFGLSDAADRTAKTYSGGMRRRLDLAACLVGEPRVIFLDEPTTGLDPNAREPVVDIIRG
EVRRGVTVLLTTQYLEEADQLADDIVVVDQGSVVARGTPETLKREVGRPTLQVRPADGAQ
VDRVAALVSEVIGEHTSDGRVVKARVRDTAAMSAIVRRLDDAGIEVAELSVREPSLDEVF
KQLTVTGAAV

>putative drug resistance ABC transporter ATP-binding protein [PokABC1]
GTGACATCCGCGATCCAGGCCCGTGGTCTGGCCAAACGCTTCGGTGAGACGGCCGCGCTC
ACCTCCGTCGACCTCGATGTCGAGGCGGGGACCGTGCTCGGGCTGCTGGGCCCCAACGGC
GCGGGCAAGACCACGGTCGTCCGTATCCTCGCCACCCTTCTACGGCCCGATGCCGGACAG
GCCTTCATCCACGGCCACGACGTGACCAAGTCCCCGGACCGGGTCCGCGAGATCATCGGC
CTCACCGGCCAGTTCGCGGCGGTCGACGACAGCCTCACCGGCGTCGAGAACCTGCTGTTC
CTCGGCCGGCTGCTGGGCAAGTCCCGTGCCGACGCGCGCGGTACGGCGCTGAGCCTGCTC
GAACGGTTCGGGCTGTCCGACGCGGCGGACCGCACCGCCAAGACGTACTCCGGCGGTATG
CGCAGGCGGCTGGACCTGGCGGCCTGCCTGGTGGGCGAGCCGCGGGTGATCTTCCTGGAC
GAGCCGACCACGGGGCTGGACCCCAATGCCCGCGAACCAGTTGTGGACATCATCCGCGGC
GAGGTCCGGCGCGGGGTGACGGTGCTGCTGACGACCCAGTACCTGGAGGAGGCCGACCAG
CTCGCCGACGACATCGTGGTCGTCGACCAGGGCTCGGTCGTGGCTCGAGGAACCCCTGAG
ACGCTCAAGCGGGAGGTCGGCAGGCCCACTCTCCAGGTGCGTCCCGCGGACGGTGCGCAG
GTGGACCGGGTGGCCGCGCTGGTGTCCGAGGTGATCGGCGAGCACACGAGCGACGGCCGG
GTGGTCAAGGCGCGGGTGCGGGACACGGCGGCGATGTCGGCGATCGTGCGGCGGCTCGAC
GACGCCGGGATCGAGGTCGCCGAACTCTCGGTGCGGGAACCGAGCCTCGACGAGGTCTTC
AAGCAGCTGACAGTGACGGGAGCGGCGGTATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR003439 ABC transporter-like (Domain) PS50893 ABC_TRANSPORTER_2 PF00005 ABC_tran IPR003593 AAA+ ATPase domain (Domain) SM00382 AAA IPR005894 Daunorubicin resistance ABC transporter ATP-binding subunit (Family) TIGR01188 DrrA IPR017871 ABC transporter, conserved site (Conserved_site) PS00211 ABC_TRANSPORTER_1 IPR025302 Domain of unknown function DUF4162 (Domain) PF13732 DUF4162
SignalP	No significant hit
TMHMM	No significant hit

Polk_00060 Polk_00080