Polk_00060 : CDS information

Location

Organism	Streptomyces diastatochromogenes
Strain	Tü6028
Entry name	Polyketomycin
Contig	FJ483966
Start / Stop / Direction	7,910 / 7,101 / - [in whole cluster] 7,910 / 7,101 / - [in contig]
Location	complement(7101..7910) [in whole cluster] complement(7101..7910) [in contig]
Type	CDS
Length	810 bp (269 aa)

Annotation

Category	4.3 transport
Product	putative drug resistance ABC transporter permease protein
Product (GenBank)	PokABC2
Gene
Gene (GenBank)	pokABC2
EC number
Keyword
Note
Note (GenBank)	putative ABC transporter transmembrane component
Reference	ACC C0JWA1 PmId [19266534] Organisation of the biosynthetic gene cluster and tailoring enzymes in the biosynthesis of the tetracyclic quinone glycoside antibiotic polyketomycin. (Chembiochem. , 2009) comment polyketomycin生合成clusterの解析論文。機能推定は配列解析から。 pokABC2: ABC transporter, transmembrane component おそらく産生細胞外へのpolyketomycin輸送に関連する。
Related Reference	ACC O86917 PmId [9696745] A silent ABC transporter isolated from Streptomyces rochei F20 induces multidrug resistance. (J Bacteriol. , 1998) comment Blast 39th, id39%, 7e-48 Streptomyces rochei_ORF2 ORF2 protein Streptomyces rochei F20で、Msr-ORF1とMsr-ORF2は、ABC transporter superfamily のメンバーであるATP-binding proteinとtransmembrane proteinに各々似ていた。msrを破壊しても形質変化は認められず、行った実験条件では発現が検出されず、inducerの発見には至っていない。msrのプロモーター領域をexpressing promoterに入れ替えたプラスミドを作成し、Streptomyces lividans TK21に形質転換させると、薬剤や化学物質(doxorubicin, oleandomycin, erythromycin, tetracycline, spiramycin, streptothricin, tobramycin, novomycin, streptomycin, chloramphenicol, phenol, pentachlorophenol, tetrachlorohydroquinone, ethidium bromide)に抵抗性を示した
Related Reference	ACC P32011 NITE Adria_00140 PmId [1924314] A bacterial analog of the mdr gene of mammalian tumor cells is present in Streptomyces peucetius, the producer of daunorubicin and doxorubicin. (Proc Natl Acad Sci U S A. , 1991) [9006006] Expression and characterization of DrrA and DrrB proteins of Streptomyces peucetius in Escherichia coli: DrrA is an ATP binding protein. (J Bacteriol. , 1997) [15709779] Biochemical characterization of domains in the membrane subunit DrrB that interact with the ABC subunit DrrA: identification of a conserved motif. (Biochemistry. , 2005) [19063901] Translational coupling controls expression and function of the DrrAB drug efflux pump. (J Mol Biol. , 2009) [20158521] Daunorubicin efflux in Streptomyces peucetius modulates biosynthesis by feedback regulation. (FEMS Microbiol Lett. , 2010) comment Blast 67th, id36%, 1e-44 Streptomyces peucetius_drrB Daunorubicin/doxorubicin resistance ABC transporter permease protein drrB -- [PMID: 1924314](1991) DrrAは排出ABC transporterのATP結合タンパク質。Mg2+依存的にATP (or GTP) を結合。 doxorubicin(DXR) or daunorubicin(DNR)の添加で活性が上昇。 DrrBはABC-2 type transporterといわれているもの。 -- さらにいくつかの続報がでており、DrrABはATP依存的なDXRとDNRの排出ポンプとされている(PMID: 15709779)。上記著者らでの最新の続報は[PMID: 19063901](2009) -- 違うグループでも報告あり。上記既報をrefとして採用している。 [PMID: 20158521](2010) drrAB(-)mutantはDNRに高い感受性あり。このmutantはwildと比べて、薬剤産生が10倍減。 key regulator dnrI and polyketide synthase gene dpsAの発現が激減。 regulatory genes dnrO and dnrNの発現は増。これらの結果から細胞内DNR濃度に基づくFeedback regulationを提唱。

Sequence

>putative drug resistance ABC transporter permease protein [PokABC2]
MTAITTTEEAPQVRRRGPGSAVRQSATVAWRQLVQIKHSPETLVEVGIQPVIFVFLFAFV
LAGQMAGSREAYLQFVVPGLIVQSTVLVTARTAIGMNTDVTKGLFDRFRSLPISRTAPLI
GRILADYVMLVWSVVLLLAFGMLLGFRIETNWGQLIPMFLLLLAFSFALSWASVLAGLLS
KDPESVQALAFGLMLPLTFISDAFVRVETMPGWLQAVVKLNPVTMLADSIRGLLVGGPVG
QPVTKTLIAAAVLLVVFAPLALRAYHRER

>putative drug resistance ABC transporter permease protein [PokABC2]
ATGACGGCCATCACCACGACCGAGGAGGCGCCGCAGGTGCGGCGTCGCGGGCCCGGATCC
GCCGTGCGGCAGAGCGCGACGGTGGCCTGGCGACAGCTCGTGCAGATCAAGCACAGCCCG
GAGACGCTGGTGGAGGTCGGCATCCAGCCGGTCATCTTCGTCTTCCTCTTCGCCTTCGTG
CTCGCCGGTCAGATGGCCGGCTCGCGTGAGGCCTATCTGCAGTTCGTCGTCCCGGGGCTG
ATCGTGCAGAGCACCGTCCTGGTCACCGCGCGCACGGCCATCGGGATGAACACCGATGTC
ACCAAGGGTCTCTTCGACCGCTTCCGCAGCCTGCCGATCTCGCGTACGGCACCGCTCATC
GGCAGGATCCTGGCGGACTACGTGATGCTGGTCTGGTCCGTGGTGCTGCTGCTCGCCTTC
GGCATGCTGCTGGGTTTCCGGATCGAGACCAACTGGGGTCAGCTGATCCCGATGTTCCTG
CTGCTCCTGGCGTTCTCGTTCGCGCTGTCCTGGGCCTCGGTGCTGGCCGGGCTGCTCTCC
AAGGACCCGGAGAGCGTGCAGGCCCTGGCCTTCGGGCTCATGCTGCCGCTGACGTTCATC
AGTGACGCCTTCGTGCGGGTGGAGACCATGCCGGGCTGGCTCCAGGCGGTCGTCAAGCTC
AACCCGGTGACCATGCTGGCCGATTCGATCCGCGGTCTCCTGGTCGGCGGTCCGGTGGGG
CAGCCCGTGACGAAGACGCTGATCGCCGCGGCGGTCCTCCTCGTGGTCTTCGCCCCGCTG
GCCCTGCGCGCCTACCACCGTGAACGGTAA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000412 ABC-2 (Family) PS51012 ABC_TM2 IPR013525 ABC-2 type transporter (Domain) PF01061 ABC2_membrane
SignalP	Eukaryota
TMHMM	Transmembrane 6

Polk_00050 Polk_00070