Polk_00050 : CDS information

Location

Organism	Streptomyces diastatochromogenes
Strain	Tü6028
Entry name	Polyketomycin
Contig	FJ483966
Start / Stop / Direction	7,088 / 5,940 / - [in whole cluster] 7,088 / 5,940 / - [in contig]
Location	complement(5940..7088) [in whole cluster] complement(5940..7088) [in contig]
Type	CDS
Length	1,149 bp (382 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative glycosyltransferase
Product (GenBank)	PokGT2
Gene
Gene (GenBank)	pokGT2
EC number
Keyword
Note
Note (GenBank)	putative glycosyltrasnferase
Reference	ACC C0JWA0 PmId [19266534] Organisation of the biosynthetic gene cluster and tailoring enzymes in the biosynthesis of the tetracyclic quinone glycoside antibiotic polyketomycin. (Chembiochem. , 2009) comment polyketomycin生合成clusterの解析論文。機能推定は配列解析から。 pokGT2: glycosyltransferase
Related Reference	ACC Q9ZGB8 NITE Lando_00220 PmId [11755402] Elucidation of the function of two glycosyltransferase genes (lanGT1 and lanGT4) involved in landomycin biosynthesis and generation of new oligosaccharide antibiotics. (Chem Biol. , 2001) [16039521] Iteratively acting glycosyltransferases involved in the hexasaccharide biosynthesis of landomycin A. (Chem Biol. , 2005) comment Blast 4th, id39%, 6e-51 Streptomyces cyanogenus_lanGT1 Glycosyl transferase homolog [DoBISCUIT]D-olivose glycosyltransferase --- [PMID:11755402](2001) landomycinとurdamycinのglycosyltransferase gene cassettesで発現実験、産物解析。 LanGT1は、他のD-olivoseの4-OH基へ2つ目のD-olivoseを結合するD-olivosyltransferaseである。 LanGT4がD-olivoseの3-OH基にL-rhodinoseを付着するL-rhodinosyltransferaseである。 LanGT4の基質特異性はゆるい。 --- [PMID:16039521](2005) landomycin E(糖側鎖がtrisaccharide: -D-olivose-D-olivose-L-rhodinose)は、wild株でlandomycin Aへ変換されるが、lanGT1 mutantではlandomycin I (tetrasaccharide: -D-olivose-D-olivose-L-rhodinose-D-olivose)を蓄積。これは5番目の糖の移動がLanGT1の不在下で起こることができないことを示す。したがって、LanGT1はこれまでに示されている2番目に加えて、5番目のglycosyltransferも触媒する。 - Significanceでは2番目と4番目と書いてあるが、内容を確認すると4番目→5番目の間違い。また、同著者らの後発の論文ではlandomycin I はD-olivoseひとつのみを持ち、このtetrasaccharideを持つものはlandomycin Jとされている。

Sequence

>putative glycosyltransferase [PokGT2]
MRVLVVALVPSHLMSMVPVAWALRSAGHEVLVAGGAPVVERAAAAGLSGAVVSEPPGRPV
RRSAAPSGLPGAGPDWALLQERWRQRVDGVLDEHLDVARRWRPDLVLVDPIEFSGLIVAA
ALRVPAVLHRWGPDRISSQSVPRAVEALGEVAARRGVDSGPALPSLVLDPCPPSLQCPEA
SAAQPVRFVPFNGAGSPPRWAPRPDAGRRLCVSFGGETPMLTRPEIWDALIRELAAVRDM
ESVVTAVPHDAAGLPASVHAPGAVPLDLFLGDCDALLHHGGAGTALTGLAFGVPQLVLAQ
SNPSWAAVGERVAARGAGVVLDLDAAMSADSSAGLRATVESVLSAPGYRSAAESLADEIR
RLPAPSDVVPLLAELAAAPVGR

>putative glycosyltransferase [PokGT2]
ATGAGGGTGCTGGTCGTCGCCCTGGTACCGAGTCATCTGATGTCGATGGTCCCGGTCGCC
TGGGCCCTGCGGTCGGCCGGGCACGAGGTCCTGGTCGCCGGCGGCGCTCCCGTGGTGGAG
CGCGCCGCGGCGGCCGGTCTGTCGGGCGCCGTGGTGAGCGAGCCACCGGGCCGGCCGGTA
CGGCGGTCGGCGGCGCCCTCAGGGCTGCCCGGCGCCGGTCCGGACTGGGCGCTGCTCCAG
GAGAGGTGGCGACAGCGCGTCGACGGCGTGCTCGACGAGCATCTGGACGTCGCCCGCCGG
TGGCGGCCGGATCTGGTGCTGGTGGATCCGATCGAGTTCAGCGGTCTGATCGTGGCGGCC
GCCCTGCGGGTGCCGGCCGTGCTGCACCGCTGGGGGCCGGACCGGATCAGCTCCCAGTCC
GTTCCGCGGGCCGTCGAGGCGCTCGGCGAGGTGGCCGCCCGGCGCGGTGTGGACTCGGGT
CCGGCGCTGCCCTCGCTGGTGCTGGACCCGTGCCCGCCGAGTCTGCAGTGCCCGGAGGCC
TCGGCGGCCCAGCCGGTGCGCTTCGTGCCCTTCAACGGGGCGGGTTCACCGCCTCGTTGG
GCGCCGCGGCCGGACGCGGGCAGGCGCCTGTGCGTCAGCTTCGGCGGCGAGACCCCGATG
CTGACGCGGCCGGAGATCTGGGACGCGCTGATCCGTGAGCTCGCCGCCGTGCGGGACATG
GAGTCGGTCGTCACCGCGGTCCCGCACGACGCGGCCGGTCTGCCCGCCTCTGTGCACGCG
CCGGGCGCGGTTCCGCTGGACCTGTTCCTCGGTGACTGCGACGCGCTGCTGCATCACGGC
GGCGCGGGGACGGCCCTGACCGGTCTGGCCTTCGGGGTGCCGCAGCTGGTCCTCGCCCAG
TCGAACCCTTCGTGGGCGGCGGTCGGCGAGCGCGTCGCGGCGCGCGGGGCAGGCGTGGTC
CTCGACCTCGACGCGGCTATGAGTGCGGACTCTTCGGCGGGGCTGCGGGCGACGGTGGAG
TCGGTGCTCTCCGCTCCCGGTTACCGCTCCGCCGCCGAGTCGCTGGCCGACGAGATCAGG
CGGCTCCCGGCGCCGAGCGACGTGGTGCCGTTGCTCGCGGAGCTGGCGGCGGCTCCTGTG
GGCCGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR010610 Domain of unknown function DUF1205 (Domain) PF06722 DUF1205
SignalP	Bacteria, Gram-positive Eukaryota Bacteria, Gram-negative
TMHMM	No significant hit

Polk_00040 Polk_00060