Polk_00220 : CDS information

Location

Organism	Streptomyces diastatochromogenes
Strain	Tü6028
Entry name	Polyketomycin
Contig	FJ483966
Start / Stop / Direction	28,755 / 30,494 / + [in whole cluster] 28,755 / 30,494 / + [in contig]
Location	28755..30494 [in whole cluster] 28755..30494 [in contig]
Type	CDS
Length	1,740 bp (579 aa)

Annotation

Category	2.1 modification addition of extender units
Product	putative acetyl-CoA carboxylase carboxyltransferase subunit
Product (GenBank)	PokAC1
Gene
Gene (GenBank)	pokAC1
EC number	6.4.1.2
Keyword
Note
Note (GenBank)	putative acetyl-CoA carboxylase carboxyl transferase subunit
Reference	ACC C0JWB7 PmId [19266534] Organisation of the biosynthetic gene cluster and tailoring enzymes in the biosynthesis of the tetracyclic quinone glycoside antibiotic polyketomycin. (Chembiochem. , 2009) comment polyketomycin生合成clusterの解析論文。機能推定は配列解析から。 pokAC1: acetyl-CoA carboxylase, carboxyl transferase subunit PokAC1, AC2, AC3はおそらく、PKS伸長unitであるmalonyl-CoAを供給する。
Related Reference	ACC P0A9Q5 PmId [1355086] The dedB (usg) open reading frame of Escherichia coli encodes a subunit of acetyl-coenzyme A carboxylase. (J Bacteriol. , 1992) [1355089] The genes encoding the two carboxyltransferase subunits of Escherichia coli acetyl-CoA carboxylase. (J Biol Chem. , 1992) [16460018] The structure of the carboxyltransferase component of acetyl-coA carboxylase reveals a zinc-binding motif unique to the bacterial enzyme. (Biochemistry. , 2006) [19965770] A tale of two functions: enzymatic activity and translational repression by carboxyltransferase. (Nucleic Acids Res. , 2010) comment >for N-terminal Blast 453rd, id50%, 5e-63, N末hit E.coli_accD(dedB, usg) Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta(EC 6.4.1.2) -- [PMID: 1355086](1992) E.coli_dedB (usg)の同定。 -- [PMID:1355089](1992) 精製されたE.coli acetyl-CoA carboxylase componentのpeptide mapping 35-kDa alpha subunit 33-kDa beta subunit それぞれのgene cloning alpha: accA beta: accD (<-dedB/usg) -- [PMID:16460018](2006) S. aureus と E. coli由来carboxyltransferase subunitsの構造解析 -- [PMID:19965770](2010) acetyl-CoA carboxylaseのcarboxyltransferase subunitは、それ自身をコードするaccA and accDのmRNAsを結合することで翻訳が抑制される。これはacetyl-CoAによって解放される。この相互作用はbeta-subunit(AccD)のzinc fingerによって媒介される。
Related Reference	ACC P0ABD5 PmId [1355089] The genes encoding the two carboxyltransferase subunits of Escherichia coli acetyl-CoA carboxylase. (J Biol Chem. , 1992) [15066985] Identification and characterization of the first class of potent bacterial acetyl-CoA carboxylase inhibitors with antibacterial activity. (J Biol Chem. , 2004) [16460018] The structure of the carboxyltransferase component of acetyl-coA carboxylase reveals a zinc-binding motif unique to the bacterial enzyme. (Biochemistry. , 2006) [19965770] A tale of two functions: enzymatic activity and translational repression by carboxyltransferase. (Nucleic Acids Res. , 2010) comment >for C-terminal Blast list外、id48.28%, 4e-59, C末hit E.coli_accA Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha(EC 6.4.1.2)

Sequence

>putative acetyl-CoA carboxylase carboxyltransferase subunit [PokAC1]
MSAPAETTTPAAWWLCDHCRRPVYAKRLARSSGVCPECGHHRRLSAPERLDQLLDPGSSQ
ALGVPRTADDVLGFTDTRPYHERLAEARDRTGLDDAAIAVRGRIGGCEVAVVAMEFGFMG
GSLSGGVGELITLTAETALAERRPLVIVCASGGARMQEGLISLMQMAKTSQALLALDEAG
IPTVSVITDPVYGGVAASFATLCDVIVAEPGARMGFAGPRVIERTIGERLPENFQRAEFL
REHGQVDVVAPRQELRATLIRLLRALAPPRDTRAGRPRAEQAEPESTGTALGAPDPGLLT
RPEQVPDLDPWQAVRLARNLERPTALDYIALLVDDFQELHGDRLGGDCPATVGGVGLLDG
RPVMVIGQQKGHTAAALRERNFGMPVPAGYRKAARLMRLAAKVGIPVLTLVDTSGAFPGI
EAERGGQAVAIAENLRLMSGLPVPIVAVIVSEGGSGGALGLAVADRVLALSNAVYSVISP
EGCAAILWRDPGAAPHAARELGLHARELLAQGVVDGVVLEPEGGAQSDHRVAAERLAGAV
RPLLEELTVLTTDQLLAGRRARFRGFGSPAPAGRPAPER

>putative acetyl-CoA carboxylase carboxyltransferase subunit [PokAC1]
ATGAGCGCCCCGGCCGAGACGACCACGCCCGCGGCCTGGTGGCTGTGCGACCACTGCCGA
CGGCCTGTCTACGCCAAGCGGCTCGCCCGGTCCTCCGGTGTGTGCCCGGAGTGCGGCCAC
CACCGGAGACTGTCGGCCCCGGAGCGGCTCGACCAGCTGCTCGACCCGGGGTCGTCCCAA
GCCCTGGGCGTGCCGCGCACCGCCGACGACGTCCTCGGCTTCACGGACACCCGGCCCTAC
CACGAGCGACTCGCCGAGGCGCGCGACCGGACCGGACTCGACGACGCCGCGATCGCCGTC
CGCGGCCGGATCGGCGGTTGCGAAGTGGCGGTCGTCGCCATGGAGTTCGGCTTCATGGGC
GGCAGCCTCAGCGGCGGCGTCGGCGAACTCATCACGCTGACCGCCGAGACGGCCCTGGCC
GAACGGCGCCCGCTGGTCATCGTCTGCGCGTCCGGGGGCGCCCGGATGCAGGAGGGCCTG
ATCTCGCTGATGCAGATGGCGAAGACGAGCCAGGCCCTGCTCGCCCTCGACGAGGCCGGC
ATCCCCACCGTCTCCGTCATCACCGACCCGGTGTACGGCGGAGTGGCCGCCTCGTTCGCC
ACGCTCTGCGACGTGATCGTGGCCGAACCGGGAGCCCGCATGGGGTTCGCGGGACCGCGC
GTCATCGAGCGGACCATCGGCGAGCGGCTCCCGGAGAACTTCCAGCGCGCCGAGTTCCTC
CGCGAGCATGGTCAGGTCGACGTGGTCGCACCGCGCCAGGAACTGCGCGCGACCCTGATC
CGGCTGCTCCGCGCGCTGGCTCCACCGCGGGACACCCGGGCGGGACGTCCTCGGGCGGAA
CAGGCCGAGCCGGAGTCCACCGGCACGGCACTCGGCGCACCGGACCCCGGCCTGCTCACC
CGTCCCGAACAGGTCCCCGACCTCGACCCCTGGCAGGCGGTGCGCCTGGCGAGGAACCTC
GAACGCCCCACCGCGCTCGACTACATCGCCCTGCTCGTCGACGACTTCCAGGAACTGCAC
GGTGACCGGCTCGGCGGCGACTGCCCGGCCACCGTCGGAGGAGTCGGCCTCCTCGACGGC
CGGCCCGTGATGGTGATCGGCCAGCAGAAAGGACACACCGCGGCGGCGCTGCGAGAACGG
AACTTCGGCATGCCGGTGCCCGCCGGCTACCGCAAGGCCGCCCGGCTGATGCGCCTGGCC
GCGAAGGTGGGCATCCCGGTGCTCACCCTCGTCGACACCAGCGGTGCCTTCCCCGGCATC
GAGGCGGAGCGCGGGGGCCAGGCCGTGGCCATCGCGGAGAACCTGCGGCTGATGTCCGGG
CTGCCCGTGCCGATCGTTGCTGTGATCGTCTCGGAGGGCGGCAGCGGCGGCGCCCTGGGA
CTCGCCGTCGCCGACCGGGTGCTCGCGCTCAGCAACGCCGTCTACTCGGTGATCAGCCCG
GAGGGATGTGCCGCGATCCTGTGGCGCGATCCCGGGGCGGCACCGCATGCCGCTCGTGAA
CTCGGTCTGCACGCACGGGAGTTGCTTGCACAGGGGGTGGTCGATGGAGTCGTCCTCGAA
CCCGAGGGCGGCGCGCAGAGCGATCACCGCGTCGCGGCGGAGCGCCTCGCCGGCGCCGTA
CGGCCGCTGCTGGAGGAACTGACAGTGCTGACCACCGATCAACTGCTGGCCGGACGACGG
GCCCGCTTCCGCGGCTTCGGCAGCCCGGCACCGGCCGGCCGCCCCGCGCCGGAAAGGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000022 Carboxyl transferase (Domain) PF01039 Carboxyl_trans IPR000438 Acetyl-CoA carboxylase carboxyl transferase, beta subunit (Family) TIGR00515 AccD IPR001095 Acetyl-CoA carboxylase, alpha subunit (Family) TIGR00513 AccA PR01069 ACCCTRFRASEA IPR011762 Acetyl-coenzyme A carboxyltransferase, N-terminal (Domain) PS50980 COA_CT_NTER IPR011763 Acetyl-coenzyme A carboxyltransferase, C-terminal (Domain) PS50989 COA_CT_CTER
SignalP	Eukaryota Bacteria, Gram-negative Bacteria, Gram-positive
TMHMM	No significant hit

Polk_00210 Polk_00230