Polk_00220 Polk_00220   Polk_00240 Polk_00240

Polk_00230 : CDS information

close this sectionLocation

Organism
StrainTü6028
Entry namePolyketomycin
Contig
Start / Stop / Direction30,499 / 31,035 / + [in whole cluster]
30,499 / 31,035 / + [in contig]
Location30499..31035 [in whole cluster]
30499..31035 [in contig]
TypeCDS
Length537 bp (178 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.1 modification addition of extender units
Productputative acetyl-CoA carboxylase biotin carboxyl carrier protein subunit
Product (GenBank)PokAC3
Gene
Gene (GenBank)pokAC3
EC number6.4.1.2
Keyword
Note
Note (GenBank)
  • putative biotin carboxylase carrier protein
Reference
ACC
PmId
[19266534] Organisation of the biosynthetic gene cluster and tailoring enzymes in the biosynthesis of the tetracyclic quinone glycoside antibiotic polyketomycin. (Chembiochem. , 2009)
comment
polyketomycin生合成clusterの解析論文。

機能推定は配列解析から。
pokAC3: biotin carboxylase carrier protein

PokAC1, AC2, AC3はおそらく、PKS伸長unitであるmalonyl-CoAを供給する。
Related Reference
ACC
P0ABD8
PmId
[8631788] Purification and characterization of intact and truncated forms of the Escherichia coli biotin carboxyl carrier subunit of acetyl-CoA carboxylase. (J Biol Chem. , 1996)
[9398236] Structure of the carboxy-terminal fragment of the apo-biotin carboxyl carrier subunit of Escherichia coli acetyl-CoA carboxylase. (Biochemistry. , 1997)
comment
Blast list外、id34.86%, 2e-11
E.coli_accB
Biotin carboxyl carrier protein of acetyl-CoA carboxylase

--
[PMID: 8631788](1996)
acetyl-CoA carboxylaseのBCCP (Biotin carboxyl carrier protein)について、
intact BCCP (156aa)、C末truncated BCCP (87aa)両方を精製し、活性を比較。
biotin-accepting activityは同程度で、機能的な違いはない、との結果。

--
[PMID: 9398236](1997)
構造解析

close this sectionSequence

selected fasta
>putative acetyl-CoA carboxylase biotin carboxyl carrier protein subunit [PokAC3]
MPTPDSVATLDALLSDGRPDSLPSVLEAAKTSMIELFSEAPVPPSAVRVTVGPVTIEMDW
PGGTGAGTTAPQLAVPSQAGTAPVATPAASPTGQPPLDHIHAPTVGVFYRAPEPGAKPFV
TEGDTVVPGQQVGLIEAMKLMIPVESDRHGVIGEFLQPNAGLVEYGDPLCSLVPADGR
selected fasta
>putative acetyl-CoA carboxylase biotin carboxyl carrier protein subunit [PokAC3]
ATGCCCACGCCCGACAGCGTCGCCACCCTCGACGCCCTGCTGAGTGACGGACGCCCCGAC
AGTCTTCCCTCCGTCCTGGAGGCCGCCAAGACGAGCATGATCGAGCTCTTCTCGGAGGCT
CCCGTACCGCCGTCCGCCGTCCGCGTGACGGTCGGGCCGGTCACCATCGAGATGGACTGG
CCGGGCGGGACCGGCGCAGGCACCACGGCGCCGCAGCTCGCAGTTCCCTCGCAGGCAGGG
ACCGCACCGGTCGCGACGCCCGCGGCGTCGCCGACCGGACAGCCCCCGCTCGACCACATC
CACGCCCCGACGGTCGGGGTCTTCTACCGCGCGCCCGAACCGGGTGCCAAACCCTTCGTG
ACCGAGGGTGACACTGTCGTCCCCGGTCAACAGGTGGGCCTCATCGAGGCGATGAAGCTG
ATGATCCCCGTCGAGTCGGACCGGCACGGTGTCATCGGCGAGTTCCTCCAGCCCAACGCG
GGCCTCGTCGAGTACGGCGACCCGTTGTGCTCCCTCGTCCCGGCCGACGGGCGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000089 Biotin/lipoyl attachment (Domain)
[99-171] 1.3e-15 PF00364
PF00364   Biotin_lipoyl
[98-172] PS50968
PS50968   BIOTINYL_LIPOYL
IPR001249 Acetyl-CoA biotin carboxyl carrier (Family)
[102-115] 1.49999977583352e-11 PR01071 [119-133] 1.49999977583352e-11 PR01071 [134-147] 1.49999977583352e-11 PR01071
PR01071   ACOABIOTINCC
IPR001882 Biotin-binding site (Binding_site)
[129-146] PS00188
PS00188   BIOTIN
IPR011053 Single hybrid motif (Domain)
[94-172] 1.50000306971104e-17 SSF51230
SSF51230   Hybrid_motif
SignalP
[1-28] 0.201 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Polk_00220 Polk_00220   Polk_00240 Polk_00240
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