Polk_00230 Polk_00230   Polk_00250 Polk_00250

Polk_00240 : CDS information

close this sectionLocation

Organism
StrainTü6028
Entry namePolyketomycin
Contig
Start / Stop / Direction31,042 / 32,454 / + [in whole cluster]
31,042 / 32,454 / + [in contig]
Location31042..32454 [in whole cluster]
31042..32454 [in contig]
TypeCDS
Length1,413 bp (470 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.1 modification addition of extender units
Productputative acetyl-CoA carboxylase biotin carboxylase subunit
Product (GenBank)PokAC2
Gene
Gene (GenBank)pokAC2
EC number6.4.1.2
6.3.4.14
Keyword
Note
Note (GenBank)
  • putative acetyl-CoA carboxylase alpha-subunit; biotin carboxylase
Reference
ACC
PmId
[19266534] Organisation of the biosynthetic gene cluster and tailoring enzymes in the biosynthesis of the tetracyclic quinone glycoside antibiotic polyketomycin. (Chembiochem. , 2009)
comment
polyketomycin生合成clusterの解析論文。

機能推定は配列解析から。
pokAC2: acetyl-CoA carboxylase, biotin carboxylase subunit

PokAC1, AC2, AC3はおそらく、PKS伸長unitであるmalonyl-CoAを供給する。
Related Reference
ACC
P49787
PmId
[7592499] The genes encoding the biotin carboxyl carrier protein and biotin carboxylase subunits of Bacillus subtilis acetyl coenzyme A carboxylase, the first enzyme of fatty acid synthesis. (J Bacteriol. , 1995)
comment
Blast 231st, id50%, 1e-118
B.subtilis_accC1
Biotin carboxylase 1(EC 6.3.4.14)
synonym: Acetyl-CoA carboxylase subunit A 1(EC 6.4.1.2)

Acetyl-CoA carboxylaseはBiotin carboxylase(BC, accC), carboxyl transferase(CT, accA and accD), biotin carboxyl carrier protein(BCCP, accB)から構成され、ATPを使ってacetyl-CoAをカルボキシル化しmalonyl-CoAを合成する。

accBとaccCをクローニング、シークエンス。
オペロンになっている。

close this sectionSequence

selected fasta
>putative acetyl-CoA carboxylase biotin carboxylase subunit [PokAC2]
MFRTVLVANRGEIALRVARACRELGIRVAVVYSTEDTDSEVVRYADEAVRIGPGSAQASY
LSIPAVIEAARRVGADAIHPGYGFLSENADFAEVCAAEGITFIGPPPEVMEALGDKSTCR
GLMADAGLPLLPGTLDPVSSPREAEAFAAEIGYPVVVKAVAGGGGRGIGVAHSADEFPAV
YRETRRHAAAVFGDGRVYLERYLQSARHVEIQILADRFGNVIHLGERDCSVQRRHQKLIE
ETPAPGLDRDVLAAMAEHAVQGAKAAGYVGAGTFEFLYDDSGRFYFMEVNCRIQVEHPVT
EMVTGVDLVHEQLWVAAGHPLRLGRRDVVPRGCAVECRINAEDADRDFTPTPGLIDELTL
PAGPFVRVDTHVRDGSRISVFYDPLLAKVAVWAPDRELALARMRRALDEVRIEGKGMHTT
AGFLRSVVESDEFSTARHDTGLVGRLLEPEPAAAGTRDPGPSQAQPPNAS
selected fasta
>putative acetyl-CoA carboxylase biotin carboxylase subunit [PokAC2]
GTGTTCCGCACCGTCCTCGTCGCCAACCGGGGCGAGATCGCCCTGCGTGTCGCGCGCGCC
TGCCGTGAACTGGGGATCAGGGTCGCCGTCGTGTACTCCACCGAGGACACCGACAGCGAG
GTCGTGCGGTACGCCGACGAGGCGGTCCGCATCGGCCCGGGGTCGGCGCAGGCGAGCTAC
CTCAGCATCCCCGCCGTCATCGAGGCCGCCCGGCGCGTCGGCGCGGACGCGATCCACCCC
GGGTACGGATTCCTCTCCGAGAACGCCGACTTCGCCGAGGTGTGCGCGGCCGAGGGCATC
ACCTTCATCGGCCCGCCCCCGGAGGTGATGGAGGCGCTGGGCGACAAGTCCACCTGCCGC
GGCCTCATGGCCGACGCGGGGCTGCCCCTGCTGCCCGGCACGCTGGACCCCGTGTCGTCG
CCGCGCGAGGCCGAGGCGTTCGCCGCCGAGATCGGCTACCCGGTGGTGGTGAAGGCGGTG
GCCGGGGGCGGCGGCCGCGGGATCGGAGTGGCGCACTCGGCCGACGAGTTCCCCGCCGTC
TACCGGGAGACGCGACGCCACGCCGCCGCCGTGTTCGGGGACGGCAGGGTCTATCTGGAG
CGCTATCTGCAGTCCGCACGGCACGTGGAGATCCAGATACTCGCCGACCGCTTCGGCAAT
GTGATCCATCTCGGCGAGCGCGACTGCTCGGTGCAGCGCCGCCATCAGAAGCTCATCGAG
GAGACCCCGGCTCCCGGGCTGGACCGCGATGTGCTCGCCGCGATGGCGGAACACGCCGTG
CAGGGAGCCAAGGCCGCCGGATACGTCGGCGCGGGGACCTTCGAGTTCCTGTACGACGAC
AGCGGACGCTTCTACTTCATGGAGGTCAACTGCCGTATCCAGGTCGAGCATCCGGTCACA
GAAATGGTCACCGGCGTCGACCTGGTCCATGAGCAGCTGTGGGTCGCCGCCGGGCATCCG
CTGCGGCTCGGCCGGCGTGACGTCGTGCCCCGCGGGTGCGCCGTCGAGTGCCGTATCAAC
GCGGAGGACGCCGACCGGGACTTCACCCCGACGCCCGGGCTCATCGACGAACTCACCCTG
CCGGCGGGCCCGTTCGTGCGCGTGGACACGCATGTGCGGGACGGCTCGCGCATCTCCGTC
TTCTACGATCCGCTGCTCGCCAAGGTCGCCGTCTGGGCCCCCGACCGGGAGCTCGCGCTG
GCCCGTATGCGCCGTGCCCTGGACGAGGTCCGGATCGAGGGCAAGGGCATGCACACCACG
GCGGGTTTCCTCCGCTCGGTCGTCGAGAGCGACGAGTTCAGCACGGCCCGGCACGACACC
GGGCTCGTCGGCCGGCTCCTGGAGCCCGAGCCGGCTGCCGCAGGGACCCGGGACCCGGGG
CCCTCGCAGGCGCAACCACCGAACGCCTCGTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR005479 Carbamoyl-phosphate synthetase large subunit-like, ATP-binding domain (Domain)
[115-322] 5.20000000000001e-72 PF02786
PF02786   CPSase_L_D2
IPR005481 Carbamoyl-phosphate synthase, large subunit, N-terminal (Domain)
[1-110] 6.00000000000001e-42 PF00289
PF00289   CPSase_L_chain
IPR005482 Biotin carboxylase, C-terminal (Domain)
[336-444] 8.59998776239544e-54 SM00878
SM00878   Biotin_carb_C
[336-443] 4.2e-35 PF02785
PF02785   Biotin_carb_C
IPR011054 Rudiment single hybrid motif (Domain)
[331-449] 3.80000697093588e-40 SSF51246
SSF51246   Rudmnt_hyb_motif
IPR011761 ATP-grasp fold (Domain)
[120-317] PS50975
PS50975   ATP_GRASP
IPR011764 Biotin carboxylation domain (Domain)
[1-448] PS50979
PS50979   BC
IPR013815 ATP-grasp fold, subdomain 1 (Domain)
[130-202] 1.5e-25 G3DSA:3.30.1490.20
G3DSA:3.30.1490.20   ATP_grasp_subdomain_1
IPR013816 ATP-grasp fold, subdomain 2 (Domain)
[204-445] 5.00000000000001e-95 G3DSA:3.30.470.20
G3DSA:3.30.470.20   ATP_grasp_subdomain_2
IPR016185 Pre-ATP-grasp fold (Domain)
[1-113] 2.69998997787809e-48 SSF52440
SSF52440   PreATP-grasp-like
[1-129] 2.99999999999998e-48 G3DSA:3.40.50.20
G3DSA:3.40.50.20   Pre-ATP_grasp
SignalP
[1-20] 0.093 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Polk_00230 Polk_00230   Polk_00250 Polk_00250
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