Polk_00250 : CDS information

Location

Organism	Streptomyces diastatochromogenes
Strain	Tü6028
Entry name	Polyketomycin
Contig	FJ483966
Start / Stop / Direction	32,469 / 33,533 / + [in whole cluster] 32,469 / 33,533 / + [in contig]
Location	32469..33533 [in whole cluster] 32469..33533 [in contig]
Type	CDS
Length	1,065 bp (354 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative glucose-1-phosphate thymidylyltransferase putative dTDP-glucose synthase
Product (GenBank)	PokS1
Gene
Gene (GenBank)	pokS1
EC number	2.7.7.24
Keyword	beta-D-amicetose alpha-L-axenose
Note
Note (GenBank)	putative NDP-glucose synthase
Reference	ACC C0JWC0 PmId [19266534] Organisation of the biosynthetic gene cluster and tailoring enzymes in the biosynthesis of the tetracyclic quinone glycoside antibiotic polyketomycin. (Chembiochem. , 2009) comment polyketomycin生合成clusterの解析論文。機能推定は配列解析から。 pokS1: NDP-glucose synthase
Related Reference	ACC Q194R4 NITE Mith_00060 PmId [9150235] Cloning and insertional inactivation of Streptomyces argillaceus genes involved in the earliest steps of biosynthesis of the sugar moieties of the antitumor polyketide mithramycin. (J Bacteriol. , 1997) comment Blast 25th, id61%, 1e-114 Streptomyces argillaceus_mtmD DTDP-glucose synthase E. coli発現タンパクで以下の2酵素の活性みている。 mtmD: glucose-1-phosphate:dTTP thymidylyl transferase mtmE: dTDP-D-glucose 4,6-dehydratase mtmD mutantはpremithramycinoneを蓄積。

Sequence

>putative glucose-1-phosphate thymidylyltransferase [PokS1]
MKALVLSGGSGTRLRPITHTLAKQLVPVGNKPVLFHGLEAIAAAGVTETGIVVGDTRAQI
EAAVGDGSRFGLEVSYLWQRQPLGLAHAVKIARDFLGDDDFVMYLGDNIVFDGITDFVER
FRVHRPAAQIMLAQVDDPRQFGVAEVSAEGRVTGLQEKPRRPRSDLAVVGVYLFSSAVHK
AVAEVEPSARGELEITDAVQWLIDHDLRTDAAVMTGCWKDTGNVPDMLEVNRLVLRSLDP
SSAGIVRDSEISGQVVVEPGARVTGSVVVGPAVIGSGATVTNSRVGPYTSIAEDCRITDS
WIRDSIVMPRASITGVRPLESSLIGQDVEIARTPEPPGAHNLVLGDRSRVRIAS

>putative glucose-1-phosphate thymidylyltransferase [PokS1]
ATGAAAGCGCTCGTGCTTTCCGGCGGTTCAGGAACCCGGCTGCGGCCGATCACCCACACG
CTGGCCAAACAACTCGTGCCCGTGGGCAACAAACCGGTGCTGTTCCACGGCCTGGAAGCG
ATCGCCGCCGCGGGGGTGACGGAGACCGGCATCGTCGTCGGGGACACCCGTGCCCAGATC
GAGGCCGCGGTCGGCGACGGATCGCGGTTCGGCCTGGAGGTCTCCTATCTGTGGCAGCGG
CAGCCGCTCGGTCTGGCCCACGCTGTGAAGATAGCCCGTGACTTCCTCGGTGACGACGAC
TTCGTGATGTATCTGGGGGACAACATCGTCTTCGACGGCATCACCGACTTCGTGGAGCGG
TTCCGGGTCCATCGGCCGGCCGCGCAGATCATGCTCGCCCAGGTCGACGACCCCCGGCAG
TTCGGCGTGGCCGAGGTGTCCGCGGAGGGCCGCGTCACGGGGCTCCAGGAAAAACCGCGC
CGGCCGCGGAGCGATCTCGCCGTCGTGGGCGTCTATCTGTTCAGCTCGGCCGTGCACAAG
GCGGTCGCCGAGGTGGAGCCCTCGGCGCGCGGCGAACTGGAGATCACGGATGCGGTGCAG
TGGCTCATCGACCACGACCTGCGGACCGACGCCGCCGTCATGACCGGCTGCTGGAAGGAC
ACGGGAAACGTGCCGGACATGCTGGAGGTCAACCGGCTGGTGCTGCGATCCCTCGATCCC
TCCAGCGCCGGGATCGTGCGCGACAGTGAGATCAGCGGCCAGGTGGTCGTCGAGCCGGGG
GCGCGGGTGACCGGTTCCGTGGTCGTCGGCCCGGCCGTGATCGGCTCGGGAGCGACCGTC
ACCAACTCGCGTGTCGGCCCGTACACCTCGATCGCGGAGGACTGCCGTATCACCGACAGC
TGGATCCGCGACTCGATCGTGATGCCCCGTGCCTCGATCACCGGTGTCCGGCCGCTGGAG
TCCTCCCTGATCGGTCAGGACGTCGAGATCGCCCGCACCCCCGAACCGCCGGGTGCACAC
AACCTCGTCCTCGGGGACCGCAGCCGGGTGCGGATCGCGTCATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005835 Nucleotidyl transferase (Domain) PF00483 NTP_transferase IPR005908 Glucose-1-phosphate thymidylyltransferase, long form (Family) TIGR01208 RmlA_long
SignalP	No significant hit
TMHMM	No significant hit

Polk_00240 Polk_00260