R1128_00120 : CDS information

Location

Organism	Streptomyces sp.
Strain	R1128
Entry name	R1128
Contig	AF293442
Start / Stop / Direction	13,754 / 15,277 / + [in whole cluster] 13,754 / 15,277 / + [in contig]
Location	13754..15277 [in whole cluster] 13754..15277 [in contig]
Type	CDS
Length	1,524 bp (507 aa)

Annotation

Category	5.1 general function
Product	putative amidase
Product (GenBank)	enantiomer selective amidase
Gene
Gene (GenBank)	zhuL
EC number
Keyword
Note
Note (GenBank)
Reference	ACC Q9F6D0 PmId [10931852] Cloning, nucleotide sequence, and heterologous expression of the biosynthetic gene cluster for R1128, a non-steroidal estrogen receptor antagonist. Insights into an unusual priming mechanism. (J Biol Chem. , 2000) comment R1128 gene clusterがあると予測されるStreptomyces sp. R1128の40kb領域をS.lividans K4-114で異種性発現し、産物としてR1128Cと新規産物HU235を検出。この領域をシークエンスして、生合成genesがすべて含まれていそうな17kb領域をサブクローニング、oligonucleotide walkingして正確な配列を得た。 zhuL: Enantiomer selective amidase R1128生合成におけるZhuLの役割は不明。 R1128Cの4-methylvaleryl部分はvalineに由来する。これに関与してvaline deaminaseとして機能する？
Related Reference	ACC Q53116 PmId [1938876] Purification, cloning, and primary structure of a new enantiomer-selective amidase from a Rhodococcus strain: structural evidence for a conserved genetic coupling with nitrile hydratase. (J Bacteriol. , 1991) comment 243rd, id34%, 1e-39 Rhodococcus sp_amdA Enantiomerase-selective amidase(EC 3.5.1.4) Enantiomerase-selective amidase(amdA) failed to express the Rhodococcus amidase in E. coli, high-level expression of the active recombinant enzyme in Brevibacterium lactofermentum.

Sequence

>putative amidase [enantiomer selective amidase]
MTLDDGAVLAPAAVLTAALRRREISSRELLDLYLARVEAVNPALNAVVTLDVERARREAA
EADRATAAGARTGPLHGLPMTVKDTLETEGLRTTAGAAELAEHVPARDADSVALLRRAGA
VVFGKTNTATYASDAQTYNPVFGTTNNPWDTSRAPGGSSGGAAAALAAGLTSLELGGELS
GSARYPAHCCGVFALRPSFGIVPMRGHIPRQPGSLKTNDMVTLAPLARSAADLDLLLDVL
ARPAADKAPAWRLDLPAPRAESLGGLRVGLWLDDPLCPVDAEVGDVLAAAVDALKGTGVA
RLADVTPVDRATHDRLYERLLQGAVSLGLPAPVYAANQAAAAALAPDDDSPRASFLRAAT
LSHRDWLAADEEREHQRARWAEVFDEFDVVLSPVAPVVATPHDQRPDLSARRITVNGAQR
PYWDIIRWTSPATAAGLPAASVPVGVARSGLPVGLQVVGPHLHDRTVTWFARRVSELLGG
FQAPPLRAAGNDGASPGSSRTEGADRA

>putative amidase [enantiomer selective amidase]
ATGACCCTCGACGACGGCGCGGTCCTCGCGCCCGCCGCCGTCCTGACGGCGGCGCTGCGC
CGCCGGGAGATCTCCAGCCGCGAACTGCTCGACCTGTACCTGGCCCGCGTCGAGGCGGTG
AACCCGGCGCTGAACGCCGTGGTCACCCTCGACGTGGAACGGGCCCGCCGCGAGGCGGCC
GAGGCGGACCGCGCGACGGCGGCCGGCGCCCGTACCGGGCCGCTGCACGGGCTGCCGATG
ACGGTCAAGGACACCCTGGAGACCGAGGGTCTGCGCACCACGGCCGGCGCCGCGGAACTG
GCCGAGCACGTGCCGGCCCGCGACGCGGACTCGGTGGCGCTGCTGCGCCGCGCGGGCGCG
GTGGTGTTCGGCAAGACCAACACCGCCACCTACGCCTCCGACGCGCAGACCTACAACCCG
GTGTTCGGCACCACCAACAACCCGTGGGACACCTCCCGCGCGCCGGGCGGGTCCTCGGGC
GGCGCGGCGGCCGCGCTCGCCGCCGGGCTGACGTCGCTGGAACTGGGCGGCGAGCTGTCC
GGTTCGGCGCGCTACCCGGCGCACTGCTGCGGCGTGTTCGCGCTGCGGCCGTCCTTCGGG
ATCGTGCCGATGCGCGGCCACATCCCGCGCCAACCGGGGTCGCTGAAGACCAACGACATG
GTGACGCTGGCGCCGCTCGCCCGCAGCGCGGCCGACCTCGACCTGCTGCTCGACGTGCTG
GCCCGGCCGGCCGCCGACAAGGCGCCCGCGTGGCGCCTGGACCTGCCCGCGCCGCGCGCC
GAATCGCTGGGCGGGCTGCGCGTGGGGCTGTGGCTGGACGACCCGCTGTGCCCGGTCGAC
GCCGAGGTCGGCGACGTGCTGGCGGCGGCGGTCGACGCCCTCAAGGGCACCGGCGTCGCA
CGGCTGGCGGACGTCACGCCGGTCGACCGCGCGACGCACGACCGGCTCTACGAGCGGCTG
TTGCAGGGCGCGGTCAGTCTCGGACTGCCCGCCCCGGTGTACGCGGCCAACCAGGCCGCG
GCCGCCGCGCTCGCCCCCGACGACGACTCGCCGCGCGCGTCGTTCCTGCGCGCCGCGACG
CTGTCGCACCGCGACTGGCTGGCGGCCGACGAGGAGCGCGAGCACCAGCGGGCCCGTTGG
GCCGAGGTGTTCGACGAGTTCGACGTCGTCCTGTCGCCCGTCGCGCCGGTCGTCGCGACG
CCGCACGACCAGCGTCCCGACCTGTCCGCGCGGCGGATCACCGTCAACGGCGCCCAACGG
CCCTACTGGGACATCATCCGCTGGACCAGCCCGGCCACCGCGGCCGGCCTGCCGGCCGCG
TCCGTCCCGGTGGGGGTGGCCCGTTCGGGCCTGCCGGTCGGGCTCCAGGTGGTCGGCCCG
CACCTGCACGACCGCACCGTCACGTGGTTCGCCCGGCGTGTCTCGGAGCTGCTCGGCGGC
TTCCAGGCCCCGCCCCTGCGCGCCGCCGGGAACGACGGGGCGTCCCCCGGATCATCACGA
ACGGAAGGAGCGGACCGTGCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000120 Amidase (Family) PF01425 Amidase IPR023631 Amidase signature domain (Domain) SSF75304 Amidase_sig_enz G3DSA:3.90.1300.10 Amidase_sig_enz
SignalP	No significant hit
TMHMM	No significant hit

R1128_00110 R1128_00130