Rimoc_00060 : CDS information

Location

Organism	Streptomyces diastaticus
Strain	108
Entry name	Rimocidin
Contig	AY442225
Start / Stop / Direction	7,777 / 9,039 / + [in whole cluster] 7,777 / 9,039 / + [in contig]
Location	7777..9039 [in whole cluster] 7777..9039 [in contig]
Type	CDS
Length	1,263 bp (420 aa)

Annotation

Category	3.3 modification reduction
Product	cytochrome P450
Product (GenBank)	RimG
Gene
Gene (GenBank)	rimG
EC number
Keyword	C-14 carboxy group formation
Note
Note (GenBank)	cytochrome P450 monooxygenase
Reference	ACC Q6T2C1 PmId [15123265] Starter unit choice determines the production of two tetraene macrolides, rimocidin and CE-108, in Streptomyces diastaticus var. 108. (Chem Biol. , 2004) [16242652] A tailoring activity is responsible for generating polyene amide derivatives in Streptomyces diastaticus var. 108. (Chem Biol. , 2005) comment [PMID: 15123265](2004) Streptomyces diastaticus var. 108におけるrimocidin と CE-108の二つのtetraene macrolidesの生産に関する文献。 rimG; resembles cytochrome P450 monooxygenase rimG自体の機能解析は行われていないが、相同性よりcytochrome P450 monooxygenaseと予想している。 -- [PMID:16242652](2005) RimG disruptant mutantでは、rimocidin and CE-108のC-14でCOOH基の代わりにCH3基を持つ化合物を産生。よってRimGは、このC-14での酸化を担う。 mutant産生noncarboxylated tetraenesは、cholesterol-containing membranesに対しての毒性が下がっている。
Related Reference	ACC B3XWK3 PmId [18640605] Increase in pladienolide D production rate using a Streptomyces strain overexpressing a cytochrome P450 gene. (J Biosci Bioeng. , 2008) comment <Cytochrome P450 monooxygenase (psmA), Streptomyces sp. A-1544, BLAST 20; id=42%> Streptomyces sp. A-1544にpladienolide B 16-hydroxylaseをエンコードするpsmAを過剰発現させると、pladienolide B より pladienolide Dへの変換が15倍高くなることを示している。また、この変換にはpsmAの下流のferredoxinをエンコードするpsmBの産物が必要であることが示されている。

Sequence

>cytochrome P450 [RimG]
MTHTEPAAPATCPVTGATAGATDTTDSTGHGTDPLVVDFPLRAPGIPFPPPEYADYRDKK
GLVLSHLPDGKRVWLVTRHEDVRAVLTNPAISSNPQHPGFPNVGETIGVPRQDQIPGWFV
GMDSPEHDRFRKALIPEFTVRRVRAMKPAIERTVDAQLDAMLAAGNTADLVADFTLPIPS
LVISALLGVPPADREFFESRTRVLVSFRAYSDEDRLAAGKDLMRYINRLIEIKKNWGGDD
IVTRLLATGAIGAHEMSGVLMLLLIAGHETTANNIALGVVTLLKNPQWIGDERAVEETLR
FHSVADLVSLRVAVEDVEIAGQHIKAGEGIVPLVAAANHDEELFACPHAFDPSRSARGHV
AFGYGVHQCLGQNLVRVEMEVAYRKLFERIPNLRLDVPEDGLNIKYDGVLYGLHELPVRW

>cytochrome P450 [RimG]
ATGACGCACACCGAACCGGCCGCGCCGGCCACCTGCCCGGTCACGGGAGCGACGGCCGGG
GCCACGGACACGACGGATTCGACCGGGCACGGAACGGACCCGCTGGTCGTCGACTTTCCG
CTGCGCGCGCCCGGCATACCCTTCCCGCCGCCCGAATACGCCGATTACCGCGACAAGAAG
GGGCTGGTGCTCTCGCACCTGCCCGACGGCAAACGGGTGTGGCTGGTCACCCGGCACGAG
GACGTACGCGCCGTCCTGACCAACCCGGCCATCAGCTCCAACCCCCAGCACCCGGGCTTT
CCCAATGTCGGCGAGACGATCGGCGTACCCAGGCAGGACCAGATACCCGGCTGGTTCGTG
GGAATGGACTCGCCCGAACACGACCGGTTCCGCAAGGCCCTCATCCCGGAGTTCACCGTG
CGGCGCGTCCGCGCGATGAAACCCGCGATCGAGCGCACGGTGGACGCGCAACTGGACGCG
ATGCTGGCCGCGGGCAACACCGCCGACCTCGTCGCCGACTTCACCCTGCCCATTCCCTCC
CTGGTGATCTCGGCACTGCTCGGCGTGCCGCCCGCCGACCGCGAGTTCTTCGAATCCAGG
ACCCGCGTCCTGGTCTCGTTCCGTGCGTACTCCGACGAGGACCGCCTGGCCGCCGGCAAG
GACCTCATGCGGTACATCAACCGGCTGATCGAGATCAAGAAGAACTGGGGCGGCGACGAC
ATCGTCACCCGGCTGCTGGCCACCGGCGCCATCGGTGCCCACGAAATGTCCGGCGTACTG
ATGCTGCTGCTCATCGCCGGCCACGAGACCACGGCCAACAACATCGCGCTCGGTGTGGTC
ACCCTGCTGAAGAATCCCCAGTGGATCGGTGACGAACGGGCCGTCGAGGAAACCCTGCGC
TTCCACTCGGTCGCCGACCTGGTGTCCCTGCGGGTGGCCGTCGAGGACGTGGAGATCGCC
GGGCAGCACATCAAGGCGGGCGAGGGCATCGTGCCGCTGGTCGCCGCCGCCAATCACGAC
GAGGAACTCTTCGCGTGCCCCCACGCGTTCGACCCCTCCCGCTCCGCCCGCGGCCATGTG
GCCTTCGGCTACGGCGTACACCAGTGCCTGGGGCAGAACCTGGTACGGGTCGAGATGGAG
GTCGCGTACCGCAAGCTCTTCGAGCGCATTCCCAACCTCCGGCTCGACGTGCCCGAGGAC
GGACTGAACATCAAGTACGACGGCGTGCTCTACGGCCTGCACGAGCTGCCCGTCCGCTGG
TGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) PR00385 P450 SSF48264 Cytochrome_P450 PF00067 p450 G3DSA:1.10.630.10 Cyt_P450 IPR002397 Cytochrome P450, B-class (Family) PR00359 BP450 IPR017972 Cytochrome P450, conserved site (Conserved_site) PS00086 CYTOCHROME_P450
SignalP	Bacteria, Gram-negative Bacteria, Gram-positive
TMHMM	No significant hit

Rimoc_00050 Rimoc_00070