Sch475_00140 Sch475_00140   Sch475_00160 Sch475_00160

Sch475_00150 : CDS information

close this sectionLocation

Organism
StrainSCC-2136
Entry nameSch 47554
Contig
Start / Stop / Direction22,100 / 20,025 / - [in whole cluster]
22,100 / 20,025 / - [in contig]
Locationcomplement(20025..22100) [in whole cluster]
complement(20025..22100) [in contig]
TypeCDS
Length2,076 bp (691 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative transketolase
Product (GenBank)putative transketolase
GeneschA15
tkt
Gene (GenBank)schA15
EC number2.2.1.1
Keyword
  • PPP
Note
Note (GenBank)
Reference
ACC
PmId
[17085966] Angucyclines Sch 47554 and Sch 47555 from Streptomyces sp. SCC-2136: cloning, sequencing, and characterization. (Mol Cells. , 2006)
comment
angucyclines Sch 47554 and Sch 47555の生合成に関連したentire gene clusterの
クローニング、シークエンス、配列解析からの特徴づけ。

SchA15(691aa): Transketolase

本文コメントなし。
Related Reference
ACC
P27302
PmId
[8396116] ( , )
[7607225] ( , )
comment
Blast id46%
Escherichia coli_tktA(tkt)
Transketolase 1(EC 2.2.1.1)

tktAとtktBはAAで74%id

---
[PMID:8396116](1993)
tktをtktAに改名している。

tktA: 主要酵素で、transketolase activityの70-90%
tktB: 活性はとても低い。transaldolaseをコードするtalAとのオペロン内にあるかもしれない。

---
[PMID:7607225](1995)
tktAにコードされるTransketolase Aの詳細な解析。
酵素活性測定あり。
73000Daのsubunitから成るHomodimerである。
ACC
P33570
PmId
[8396116] ( , )
comment
Blast id47%
Escherichia coli (strain K12)_tktB
Transketolase 2(EC 2.2.1.1)
ACC
O06811
PmId
comment
Blast id61%
Mycobacterium tuberculosis_tkt
Transketolase(EC 2.2.1.1)

構造解析およびkinetic解析を行っている。

close this sectionSequence

selected fasta
>putative transketolase [putative transketolase]
MSTQTPDSFEWTDLDRRAVDTARLLAADAVQKVGNGHPGTAMSLAPAAYTLFQKVMRHDP
ADPEWTGRDRFVLSPGHTSLTLYTQLFLSGYELELDDLKAFRTHGSKTPGHPEYGHTAGV
ETTTGPLGQGVANAVGMAMAARFERGLFDPEAPEGESPFDHTVWAIVSDGDLEEGISAEA
SSLAGHQKLGNLVFVYDDNHISIEGDTATAFSEDVLKRYEAYGWHVQRIEPALGGDIDPR
ALHEALTAAKAETGRPSIIAMRTIIAWPAPNAQNTEAAHGSALGAEEIAATKRLLGFDPE
QTFEVADEVLAHARRALDRGAEAHAAWNKQLAEWRTAQPARAKLFDRIVAGQLPEGWESA
LPAFEAGTSVATRAASGKVLQALGDVLPELWGGSADLAGSNNTTIDKTSSFLPKGNPLPE
ADPYGRTVHFGIREHSMAAEMNGIALHGNTRIYGGTFLVFSDYMRNAVRLSALMQLPVTY
VWTHDSIGLGEDGPTHQPVEHLASLRAIPGLNIVRPADANETSIAWAEILKRHATNPAPH
GLALTRQGVPTYEANPEAAKGGYVLRDSSTATPDVILVATGSEVQLAVTAREQLEAEGVG
TRVVSMPSVEWFEEQSPEYRASVLPPSVKARVAVEAGIGLTWYRYVGDHGRIVSLEHFGA
SADAKTLFAEFGFTPENVASAARESLAAVRG
selected fasta
>putative transketolase [putative transketolase]
ATGAGCACGCAGACACCTGACAGCTTTGAGTGGACCGATCTGGACCGGCGAGCCGTCGAC
ACGGCCCGTCTGCTTGCGGCGGACGCCGTGCAGAAGGTCGGCAACGGCCACCCGGGCACC
GCGATGAGCCTCGCACCGGCGGCGTACACCCTCTTTCAGAAGGTGATGCGACACGATCCC
GCGGATCCCGAGTGGACCGGCCGCGACCGCTTCGTCCTCTCCCCCGGCCACACCTCGCTG
ACCCTCTACACCCAGCTCTTCCTCTCCGGGTACGAGCTGGAGCTCGACGACCTCAAGGCC
TTCCGCACGCACGGCTCGAAGACGCCCGGACACCCGGAGTACGGGCACACGGCCGGGGTC
GAGACGACCACGGGCCCCCTCGGCCAGGGCGTCGCCAACGCGGTGGGCATGGCGATGGCC
GCCCGCTTCGAGCGCGGCCTGTTCGACCCCGAGGCTCCCGAGGGCGAGTCCCCCTTCGAC
CACACCGTCTGGGCAATCGTCTCGGACGGTGACCTGGAGGAGGGCATCTCCGCCGAGGCG
TCCTCGCTCGCCGGCCACCAGAAGCTCGGCAACCTCGTCTTCGTCTACGACGACAACCAC
ATCTCCATCGAGGGCGACACCGCCACCGCGTTCTCCGAGGACGTACTGAAGCGGTACGAG
GCGTACGGCTGGCACGTGCAGCGGATCGAGCCCGCGCTCGGCGGCGACATCGACCCGCGC
GCGCTCCACGAGGCCCTGACCGCGGCGAAGGCCGAGACCGGCCGCCCCTCGATCATCGCG
ATGCGCACGATCATCGCCTGGCCCGCGCCCAACGCCCAGAACACCGAGGCCGCGCACGGC
TCGGCGCTCGGCGCCGAGGAGATCGCGGCCACCAAGCGCCTCCTGGGCTTCGACCCGGAG
CAGACCTTCGAGGTCGCCGACGAGGTTCTCGCCCACGCCCGGCGGGCCCTCGACCGGGGT
GCCGAGGCGCACGCCGCCTGGAACAAGCAGCTGGCCGAGTGGCGCACCGCGCAGCCCGCG
CGCGCCAAGCTCTTCGACCGGATCGTGGCCGGTCAGCTCCCCGAGGGCTGGGAGTCCGCG
CTCCCGGCGTTCGAGGCGGGCACGTCCGTCGCGACCCGTGCCGCGTCCGGCAAGGTCCTC
CAGGCGCTCGGCGACGTACTCCCCGAGCTGTGGGGCGGCTCCGCCGACCTCGCCGGCTCG
AACAACACGACCATCGACAAGACCTCGTCCTTCCTCCCGAAGGGCAACCCGCTGCCGGAG
GCCGACCCGTACGGCCGAACCGTCCACTTCGGAATCCGTGAGCACTCGATGGCCGCGGAG
ATGAACGGCATCGCGCTGCACGGCAACACCCGGATCTACGGCGGCACGTTCCTCGTCTTC
TCCGACTACATGCGCAACGCCGTCCGCCTTTCGGCCCTGATGCAGCTGCCGGTGACGTAC
GTCTGGACGCACGACTCCATCGGCCTCGGCGAGGACGGCCCGACCCACCAGCCGGTCGAG
CACCTGGCCTCACTCCGCGCCATCCCGGGCCTGAACATCGTGCGCCCCGCCGACGCCAAC
GAGACGTCGATCGCCTGGGCCGAGATCCTCAAGCGGCACGCCACCAACCCGGCCCCGCAC
GGCCTCGCCCTCACCCGTCAGGGCGTGCCGACGTACGAGGCCAACCCCGAGGCGGCGAAG
GGCGGTTACGTCCTGCGCGACTCCTCCACCGCGACCCCGGACGTGATCCTCGTCGCGACC
GGCTCCGAGGTGCAGCTCGCCGTGACCGCGCGCGAGCAGTTGGAGGCCGAGGGCGTCGGA
ACGCGTGTGGTGTCGATGCCGTCCGTCGAGTGGTTCGAGGAGCAGTCGCCGGAGTACCGA
GCGAGCGTGCTTCCGCCGTCCGTGAAGGCCCGCGTGGCGGTCGAGGCGGGCATCGGCCTG
ACCTGGTACCGGTACGTCGGTGACCACGGACGCATCGTCTCCCTCGAACACTTCGGCGCC
TCCGCCGACGCCAAGACCCTGTTCGCCGAGTTCGGCTTCACCCCCGAGAACGTGGCCTCC
GCCGCGCGGGAATCTCTTGCCGCAGTCCGCGGTTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR005474 Transketolase, N-terminal (Domain)
[15-354] 6.7999999999999e-128 PF00456
PF00456   Transketolase_N
[23-43] PS00801
PS00801   TRANSKETOLASE_1
IPR005475 Transketolase-like, pyrimidine-binding domain (Domain)
[370-551] 4.99996518094122e-66 SM00861
SM00861   Transket_pyr
[369-547] 8.60000000000005e-43 PF02779
PF02779   Transket_pyr
IPR005476 Transketolase, C-terminal (Domain)
[563-678] 4.40000000000001e-10 PF02780
PF02780   Transketolase_C
IPR005478 Transketolase, bacterial-like (Family)
[17-684] TIGR00232
TIGR00232   Tktlase_bact
IPR009014 Transketolase, C-terminal/Pyruvate-ferredoxin oxidoreductase, domain II (Domain)
[551-687] 2.39999798157265e-41 SSF52922
SSF52922   Transketo_C_like
IPR015941 Transketolase-like, C-terminal (Domain)
[561-687] 1.40000000000001e-48 G3DSA:3.40.50.920
G3DSA:3.40.50.920   Transketo_C_like
IPR020826 Transketolase binding site (Binding_site)
[490-506] PS00802
PS00802   TRANSKETOLASE_2
SignalP No significant hit
TMHMM No significant hit
Sch475_00140 Sch475_00140   Sch475_00160 Sch475_00160
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