Sch475_00160 Sch475_00160   Sch475_00180 Sch475_00180

Sch475_00170 : CDS information

close this sectionLocation

Organism
StrainSCC-2136
Entry nameSch 47554
Contig
Start / Stop / Direction24,619 / 23,237 / - [in whole cluster]
24,619 / 23,237 / - [in contig]
Locationcomplement(23237..24619) [in whole cluster]
complement(23237..24619) [in contig]
TypeCDS
Length1,383 bp (460 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative glucan endo-1,3-beta-D-glucosidase
putative endo-1,3-beta-glucanase
Product (GenBank)putative endo-1,3-beta-glucanase
Gene
Gene (GenBank)schA17
EC number3.2.1.39
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[17085966] Angucyclines Sch 47554 and Sch 47555 from Streptomyces sp. SCC-2136: cloning, sequencing, and characterization. (Mol Cells. , 2006)
comment
angucyclines Sch 47554 and Sch 47555の生合成に関連したentire gene clusterの
クローニング、シークエンス、配列解析からの特徴づけ。

SchA17(460aa): Endo-1,3-beta-glucanase

本文コメントなし。
Related Reference
ACC
Q9L816
PmId
comment
Blast 6th, id77%, 1e-167
Streptomyces sioyaensis
Endo-1,3-beta-glucanase

activity plate assayに基づき、1,3-beta-glucanaseをコードする遺伝子を分離して配列解析。
N末はglycosyl hydrolase domain
C末はcarbohydrate-binding module(CBM)

全長とCBMなしのタンパクをE.coliで発現、精製、活性確認。
glycosyl hydrolase domainは1,3-beta linkageを持ったglucansの加水分解を優先的に触媒する。
soluble 1,3-beta-glucanに対する活性は、全長とglycosyl hydrolase domainで似ている。
全長だとinsoluble 1,3-beta-glucansでの活性が強いので、CBMが不溶性基質に対するS. sioyaensis 1,3-beta-glucanase活性を拡大すると示唆される。

close this sectionSequence

selected fasta
>putative glucan endo-1,3-beta-D-glucosidase [putative endo-1,3-beta-glucanase]
MRETSGTPVRRGPLRRTLIALVSVLSLAAAGASAAEADAPAPPSGWTQVFVDDFNGTAGT
GVNTSNWQYATGHGYPGGPSNWGTGEIENMTSSTNNVALDGAGNLRITPRRDAAGNWTSG
RIETNRTDFQPPAGGKLRVQSRIQMPNVTGAAARGYWPAFWMLGAPYRGNYQNWPSVGEL
DILENVQGLNTVWATMHCGSSPGGPCNETTGLGGSTACPGATCQAGFHTYGMEWDRSTSP
EEIRFYVDGINYHTVRANQVDATTWANATNHGFFIILNVAMGGGFVDAFGGGPDGATVPG
NPLVVDYVQVLQSAGGTTPPPTGNRDAYSAIQAESFDGQSGTITETTTDSGGGQNIGALA
NGDWVQYKGVNFGSTAARQFSARVASGVGGGASGLVEVRLDSRTNAPIGTFALANTGGWQ
SWRTVPTNTSGVTGTHDVYLTFTSGQPADFVNVNWFSFAR
selected fasta
>putative glucan endo-1,3-beta-D-glucosidase [putative endo-1,3-beta-glucanase]
ATGAGGGAAACTTCAGGCACACCCGTCAGACGCGGTCCACTCCGGCGCACGCTCATCGCG
CTCGTCAGCGTGCTCAGCCTGGCGGCGGCCGGGGCATCGGCGGCAGAGGCGGACGCGCCC
GCGCCGCCCAGCGGCTGGACCCAGGTCTTCGTCGACGACTTCAACGGCACGGCGGGCACC
GGCGTCAACACCTCGAACTGGCAGTACGCGACCGGCCACGGCTACCCGGGCGGGCCCTCC
AACTGGGGCACGGGCGAGATCGAGAACATGACGTCCAGCACCAACAACGTGGCGCTGGAC
GGCGCCGGGAACCTCCGGATCACCCCGCGCCGCGACGCCGCGGGCAACTGGACCTCGGGC
CGCATCGAGACCAACCGCACCGACTTCCAGCCGCCCGCCGGTGGCAAACTGCGTGTCCAG
TCCCGGATCCAGATGCCGAACGTGACGGGCGCCGCCGCCCGCGGCTACTGGCCGGCGTTC
TGGATGCTGGGCGCGCCCTACCGCGGCAACTACCAGAACTGGCCCAGCGTCGGCGAGTTG
GACATCCTGGAGAACGTCCAGGGTCTCAACACCGTGTGGGCCACGATGCATTGCGGCTCC
AGCCCGGGCGGCCCCTGCAACGAGACGACCGGCCTCGGCGGCTCGACCGCCTGCCCGGGC
GCGACCTGCCAGGCCGGCTTCCACACGTACGGCATGGAGTGGGACCGGTCGACGAGCCCG
GAGGAGATCCGCTTCTACGTCGACGGCATCAACTACCACACCGTACGGGCGAATCAGGTC
GACGCCACGACCTGGGCGAACGCCACGAACCATGGCTTCTTCATCATCCTGAACGTGGCG
ATGGGCGGCGGTTTCGTGGACGCCTTCGGCGGCGGTCCGGACGGTGCCACCGTGCCCGGC
AACCCACTCGTCGTCGACTACGTCCAGGTGCTGCAGTCCGCGGGCGGCACCACACCTCCG
CCGACCGGCAACCGTGACGCGTACAGCGCGATCCAGGCCGAGTCGTTCGACGGTCAGAGC
GGCACTATCACCGAGACCACGACCGACTCTGGCGGCGGCCAGAACATCGGCGCCCTCGCC
AACGGCGACTGGGTGCAGTACAAGGGCGTCAACTTCGGCTCCACGGCGGCCAGGCAGTTC
AGCGCCCGGGTCGCGAGCGGTGTGGGGGGCGGCGCCAGTGGCCTGGTCGAAGTCCGCCTC
GACAGCCGTACGAACGCACCGATCGGCACCTTCGCGCTCGCCAACACCGGCGGCTGGCAG
AGCTGGCGGACCGTCCCGACGAACACCAGCGGCGTCACCGGAACGCACGACGTCTATCTG
ACCTTCACGAGCGGCCAACCGGCGGACTTCGTGAACGTGAACTGGTTCAGCTTCGCCCGC
TGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000757 Glycoside hydrolase, family 16 (Domain)
[89-283] 5.5e-20 PF00722
PF00722   Glyco_hydro_16
IPR005084 Carbohydrate binding module family 6 (Domain)
[329-459] PS51175
PS51175   CBM6
[332-459] 1.99999999999999e-39 PF03422
PF03422   CBM_6
IPR006311 Twin-arginine translocation pathway, signal sequence (Conserved_site)
[1-41] PS51318
PS51318   TAT
IPR006584 Cellulose binding, type IV (Domain)
[324-460] 5.40001756046195e-61 SM00606
SM00606   CBD_IV
IPR008979 Galactose-binding domain-like (Domain)
[323-460] 1.20000117458134e-38 SSF49785
SSF49785   Gal_bind_like
IPR008985 Concanavalin A-like lectin/glucanase (Domain)
[29-314] 2.1000026783403e-52 SSF49899
SSF49899   ConA_like_lec_gl
IPR013320 Concanavalin A-like lectin/glucanase, subgroup (Domain)
[26-325] 4.49999999999996e-67 G3DSA:2.60.120.200
G3DSA:2.60.120.200   ConA_like_subgrp
SignalP
[1-34] 0.998 Signal
Eukaryota   
[1-37] 1 Signal
Bacteria, Gram-positive   
[1-34] 1 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Sch475_00160 Sch475_00160   Sch475_00180 Sch475_00180
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