Sch475_00230 : CDS information

Location

Organism	Streptomyces sp.
Strain	SCC-2136
Entry name	Sch 47554
Contig	AJ628018
Start / Stop / Direction	34,758 / 33,361 / - [in whole cluster] 34,758 / 33,361 / - [in contig]
Location	complement(33361..34758) [in whole cluster] complement(33361..34758) [in contig]
Type	CDS
Length	1,398 bp (465 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative NDP-hexose 2,3-dehydratase
Product (GenBank)	putative NDP-hexose 2, 3-dehydratase
Gene
Gene (GenBank)	schS2
EC number
Keyword	L-aculose amicetose
Note
Note (GenBank)
Reference	ACC Q2HR51 PmId [17085966] Angucyclines Sch 47554 and Sch 47555 from Streptomyces sp. SCC-2136: cloning, sequencing, and characterization. (Mol Cells. , 2006) comment angucyclines Sch 47554 and Sch 47555の生合成に関連したentire gene clusterのクローニング、シークエンス、配列解析からの特徴づけ。 SchS2(465aa): dNDP-hexose 2,3-dehydratase UrdS, Gra-ORF27に似ている。 2-deoxysugar生合成に関連した2,3-dehydrataseであると提唱されている。
Related Reference	ACC Q9ALN6 NITE Spino_00070 PmId [18345667] In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa. (J Am Chem Soc. , 2008) comment Blast 27th, id51%, 1e-125 Saccharopolyspora spinosa_spnO Probable NDP-hexose-2,3-dehydratase dTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase_SpnO dTDP-D-glucose, RfbB(dTDP-D-glucose→dTDP-4-keto-6-deoxy-D-glucose), and either NADH or NADPHと一緒にSpnO and SpnNをインキュベートし、NAD(P)Hの消費量をフォローしてtandem reactionを測定。SpnO or SpnNのどちらかを欠いた場合、消費が見られなかった。 dTDP-3,4-diketo-2,6-dideoxy-D-glucoseは不安定で急速にdTDP and maltolに分解してしまうため、SpnOのみの触媒効率は定量できていない。 -- SpnNはdTDP-3,4-diketo-2,6-dideoxy-D-glucose 3-ketoreductaseであることが他で報告されている。

Sequence

>putative NDP-hexose 2,3-dehydratase [putative NDP-hexose 2, 3-dehydratase]
MLSHTLRSRGEAALADRLALSAATTEGSQLATADFHGWLAERGRANAFRVDRIPFAELQG
WSFEESTGNLVHRSGRFFTVEGLHVTERDGPYGDGPYADWYQPIIKQPEVGILGILVKEF
DGVPHFLMQAKMEPGNPNLLQLSPTVQATRSNYTKAHQGADVKYIEHFVGPGRGRVVADA
LQSEHGSWFFRKSNRNMIVEAVGDVPLLDDFCWLTLGQIGELLHQDNVVNMDSRTVLSCL
PLPATASGALLTDIELLSWITGERARHDVHAERVPLAGLPGWRQDEMTVEHEEGRYFNVV
GVAVQAGNREVTSWTQPLFEPVGTGVTAFLTQEFDGVPHVLVHARVEGGFLDTVELGPTV
QYTPGNYAHLAEKDRPPFLDSVLDASPDRVRYGAVHSEEGGRFRNAESRYLIIDAAEHDA
PLDPPPGYTWVTPGQLTWLVRHGHYLNVQVRTLLACLNALPARAR

>putative NDP-hexose 2,3-dehydratase [putative NDP-hexose 2, 3-dehydratase]
ATGCTGTCCCACACCCTTCGATCGCGCGGCGAGGCCGCCCTGGCCGACCGGCTCGCGCTG
TCGGCCGCCACCACCGAGGGTTCCCAGCTGGCGACCGCCGACTTCCACGGCTGGCTCGCC
GAGCGCGGCCGGGCCAACGCGTTCCGCGTGGACCGCATCCCGTTCGCCGAGTTGCAGGGC
TGGTCGTTCGAGGAGTCCACCGGAAACCTGGTGCACCGCAGCGGCCGGTTCTTCACGGTC
GAGGGCCTGCACGTCACCGAGCGGGACGGCCCCTACGGCGACGGCCCGTACGCCGACTGG
TACCAGCCCATCATCAAGCAACCCGAGGTCGGCATCCTGGGCATCCTCGTCAAGGAGTTC
GACGGGGTGCCGCACTTCCTGATGCAGGCCAAGATGGAGCCCGGCAACCCGAATCTGCTC
CAGCTCTCCCCCACGGTCCAGGCCACCCGCAGCAACTACACCAAGGCCCACCAGGGCGCG
GACGTGAAGTACATCGAGCACTTCGTCGGCCCCGGCCGCGGCCGGGTCGTCGCGGACGCC
CTGCAGTCCGAGCACGGCTCCTGGTTCTTCCGGAAGTCCAACCGCAACATGATCGTGGAG
GCGGTCGGCGACGTCCCGCTCCTCGACGACTTCTGCTGGCTCACCCTCGGTCAGATCGGC
GAGCTGCTGCACCAGGACAATGTCGTCAACATGGACTCACGGACCGTGCTGTCCTGCCTG
CCCCTGCCGGCGACGGCGTCCGGCGCACTGCTCACCGACATCGAGCTGCTGTCCTGGATC
ACCGGGGAGCGCGCCCGGCACGACGTGCACGCCGAACGCGTACCGCTGGCCGGACTGCCC
GGCTGGCGGCAGGACGAGATGACCGTCGAGCACGAGGAGGGCCGCTACTTCAACGTCGTC
GGGGTGGCCGTCCAGGCCGGCAACCGCGAGGTCACCAGCTGGACCCAACCCCTGTTCGAG
CCGGTCGGCACCGGCGTCACCGCCTTCCTCACCCAGGAGTTCGACGGCGTCCCGCACGTC
CTGGTGCACGCCCGGGTCGAGGGCGGCTTCCTGGACACCGTGGAGCTGGGCCCCACCGTC
CAGTACACGCCGGGCAACTACGCCCACCTCGCGGAGAAGGACCGCCCGCCCTTCCTGGAC
TCGGTCCTCGACGCGTCGCCCGACCGTGTCCGCTACGGTGCCGTGCACTCGGAGGAGGGC
GGCCGGTTCCGCAACGCCGAGAGCCGCTATCTGATCATCGACGCCGCCGAGCACGACGCG
CCGCTCGACCCACCGCCCGGCTACACATGGGTCACCCCCGGCCAGCTCACCTGGCTCGTC
CGCCACGGCCACTATCTCAACGTGCAGGTCCGCACGCTGCTGGCCTGCCTCAACGCACTC
CCGGCGAGGGCACGTTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005212 NDP-hexose 2,3-dehydratase (Domain) PF03559 Hexose_dehydrat
SignalP	Eukaryota
TMHMM	No significant hit

Sch475_00220 Sch475_00240