Sch475_00230 Sch475_00230   Sch475_00250 Sch475_00250

Sch475_00240 : CDS information

close this sectionLocation

Organism
StrainSCC-2136
Entry nameSch 47554
Contig
Start / Stop / Direction37,175 / 35,871 / - [in whole cluster]
37,175 / 35,871 / - [in contig]
Locationcomplement(35871..37175) [in whole cluster]
complement(35871..37175) [in contig]
TypeCDS
Length1,305 bp (434 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative pyridoxamine 5'-monophosphate-dependent NDP-hexose C-3 dehydratase
Product (GenBank)putative NDP-hexose 3-dehydratase
Gene
Gene (GenBank)schS3
EC number
Keyword
  • amicetose
Note
Note (GenBank)
Reference
ACC
PmId
[17085966] Angucyclines Sch 47554 and Sch 47555 from Streptomyces sp. SCC-2136: cloning, sequencing, and characterization. (Mol Cells. , 2006)
comment
angucyclines Sch 47554 and Sch 47555の生合成に関連したentire gene clusterの
クローニング、シークエンス、配列解析からの特徴づけ。

SchS3(434aa): NDP-hexose 3-dehydratase

UrdQ(dNDP-glucose 3-dehydratase)に似ている。
Related Reference
ACC
Q9ALN8
NITE
Spino_00050
PmId
[17076492] Characterization of SpnQ from the spinosyn biosynthetic pathway of Saccharopolyspora spinosa: mechanistic and evolutionary implications for C-3 deoxygenation in deoxysugar biosynthesis. (J Am Chem Soc. , 2006)
[18345667] In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa. (J Am Chem Soc. , 2008)
comment
Blast 21st, id67%, 1e-158
Saccharopolyspora spinosa_spnQ
probable NDP-hexose-3,4-dehydratase

--
[PMID: 17076492](2006)
SpnQをクローニング、過剰発現、精製して、"ColD-like" conditions と "E1-like" conditionsの両方で触媒活性をテスト。
E3のような特異的reductaseを必要とせず、一般的なferredoxin/ferredoxin reductase またはflavodoxin/flavodoxin reductaseと効率的に働く。

--
[PMID: 18345667](2008)
dTDP-D-forosamine形成に関連する5つの酵素SpnO, N, Q, R, and Sの生化学的機能解明。
SpnQのsteady-state kinetic parameters、基質特異性を調査。

close this sectionSequence

selected fasta
>putative pyridoxamine 5'-monophosphate-dependent NDP-hexose C-3 dehydratase [putative NDP-hexose 3-dehydratase]
MSDPKETVLDAVRAYHEVSTPAREFVPGTTEIWPSGAVLEVEDRVALVEAALDMRIAAGR
SQRKFESAFARRMKRRKAHLTNSGSSANLLAVSALTSSHLEDRRLRPGDEIITVAAGFPT
TVNPILQNGLVPVFVDVDLTTYNATADKVAAAIGPKTRAIIIAHALGNPFEVTAIAQLAE
EHDLFLIEDNCDAVGSLYDGQLTGTFGDMTTVSFYPAHHLTMGEGGCVLTSNLALARIVE
SLRDWGRDCWCEPGENDKCLKRFKYQMGTLPAGYDHKYIFSHVGYNLKATDIQAALGLTQ
LAKLDDFIDARKRNWRRLREGLDGVPGLILPEATPRSDPSWFGFVITVDPEAPFGRAELV
SHLEDRKIGTRRLFAGNLTRHPAYIDQPHRVVGDLTNSDIITEHTFWIGVYPALTDEMLD
YVTASIKEFVAARG
selected fasta
>putative pyridoxamine 5'-monophosphate-dependent NDP-hexose C-3 dehydratase [putative NDP-hexose 3-dehydratase]
ATGAGCGACCCCAAGGAAACGGTGCTCGACGCGGTCCGCGCGTACCACGAAGTCAGCACA
CCGGCACGGGAGTTCGTGCCCGGCACGACGGAGATCTGGCCCTCGGGCGCGGTCCTCGAG
GTGGAGGACCGGGTGGCGCTGGTGGAAGCCGCCCTCGACATGCGGATCGCCGCCGGGCGC
AGCCAGCGCAAATTCGAGTCGGCGTTCGCCCGGCGGATGAAGCGGCGCAAGGCCCATCTC
ACCAACTCCGGCTCCTCGGCGAACCTCCTCGCCGTCTCGGCGCTCACCTCGTCCCATCTG
GAGGACCGGCGGCTGAGGCCGGGCGACGAGATCATCACCGTCGCGGCGGGCTTCCCCACC
ACGGTCAACCCGATCCTGCAGAACGGGCTCGTACCGGTCTTCGTCGATGTGGACCTGACG
ACGTACAACGCGACCGCGGACAAGGTGGCCGCCGCCATCGGGCCCAAGACCCGGGCGATC
ATCATCGCGCACGCGCTCGGCAACCCCTTCGAGGTCACCGCGATCGCCCAACTCGCCGAG
GAACACGACCTGTTCCTCATCGAGGACAACTGTGACGCGGTCGGCTCGCTGTACGACGGA
CAGCTCACCGGCACCTTCGGCGACATGACCACGGTCAGCTTCTACCCGGCGCACCACCTC
ACGATGGGCGAGGGCGGCTGTGTGCTGACCTCGAACCTGGCGCTCGCCCGGATCGTGGAG
TCGCTGCGGGACTGGGGCCGGGACTGCTGGTGCGAGCCGGGCGAGAACGACAAGTGCCTC
AAGCGGTTCAAGTACCAGATGGGCACCCTGCCGGCCGGCTACGACCACAAGTACATCTTC
TCGCACGTCGGTTACAACCTGAAGGCCACCGACATCCAGGCCGCCCTCGGTCTGACCCAG
CTCGCCAAGCTCGACGACTTCATCGACGCCCGCAAGCGCAACTGGCGGCGGCTGCGCGAG
GGCCTGGACGGCGTACCCGGGCTGATCCTGCCCGAGGCCACGCCCCGCTCCGACCCGAGC
TGGTTCGGATTCGTCATCACCGTGGACCCCGAAGCCCCGTTCGGCCGGGCGGAGTTGGTG
TCCCACCTGGAGGACCGCAAGATCGGCACCCGGCGGCTGTTCGCCGGCAATCTCACCCGG
CATCCCGCCTACATCGACCAGCCCCACCGTGTGGTCGGCGACCTGACCAACAGCGACATC
ATCACCGAGCACACGTTCTGGATCGGGGTCTACCCGGCGCTCACCGACGAGATGCTCGAC
TACGTGACCGCCTCGATCAAGGAGTTCGTGGCCGCGCGCGGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000653 DegT/DnrJ/EryC1/StrS aminotransferase (Family)
[1-434] 2.39999798157263e-112 PIRSF000390
PIRSF000390   PLP_StrS
[44-426] 8.29999999999993e-100 PF01041
PF01041   DegT_DnrJ_EryC1
IPR015421 Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (Domain)
[32-302] 4.40000000000003e-69 G3DSA:3.40.640.10
G3DSA:3.40.640.10   PyrdxlP-dep_Trfase_major_sub1
IPR015422 Pyridoxal phosphate-dependent transferase, major region, subdomain 2 (Domain)
[303-427] 4.70000000000003e-51 G3DSA:3.90.1150.10
G3DSA:3.90.1150.10   PyrdxlP-dep_Trfase_major_sub2
IPR015424 Pyridoxal phosphate-dependent transferase, major domain (Domain)
[29-433] 1.19999063421924e-96 SSF53383
SSF53383   PyrdxlP-dep_Trfase_major
SignalP No significant hit
TMHMM No significant hit
Sch475_00230 Sch475_00230   Sch475_00250 Sch475_00250
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