Sch475_00250 : CDS information

Location

Organism	Streptomyces sp.
Strain	SCC-2136
Entry name	Sch 47554
Contig	AJ628018
Start / Stop / Direction	38,173 / 37,175 / - [in whole cluster] 38,173 / 37,175 / - [in contig]
Location	complement(37175..38173) [in whole cluster] complement(37175..38173) [in contig]
Type	CDS
Length	999 bp (332 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative NDP-hexose C-4 ketoreductase
Product (GenBank)	putative NDP-hexose 4-keto reductase
Gene
Gene (GenBank)	schS4
EC number
Keyword	amicetose
Note	The N-terminal position was modified from original INSDC entry.
Note (GenBank)
Reference	ACC Q2HR53 PmId [17085966] Angucyclines Sch 47554 and Sch 47555 from Streptomyces sp. SCC-2136: cloning, sequencing, and characterization. (Mol Cells. , 2006) comment angucyclines Sch 47554 and Sch 47555の生合成に関連したentire gene clusterのクローニング、シークエンス、配列解析からの特徴づけ。 SchS4(319aa): NDP-hexose 4-keto reductase deoxysugar生合成に関与したurd genesと相同性のあるSchS1-S8の中で、SchS4はUrdZ3と相同性あるが他と比べてid低い。これは立体特異性の違いの証拠かもしれない。 sch cluster内には他にKR候補がなく、D- and L-amicetose形成の際のketoreductionをSchS4が担うかもしれない。
Related Reference	ACC O33708 NITE Adria_00200 PmId [9209071] Cloning and characterization of the Streptomyces peucetius dnmZUV genes encoding three enzymes required for biosynthesis of the daunorubicin precursor thymidine diphospho-L-daunosamine. (J Bacteriol. , 1997) [10631513] A two-plasmid system for the glycosylation of polyketide antibiotics: bioconversion of epsilon-rhodomycinone to rhodomycin D. (Chem Biol. , 1999) [9447597] Production of the antitumor drug epirubicin (4'-epidoxorubicin) and its precursor by a genetically engineered strain of Streptomyces peucetius. (Nat Biotechnol. , 1998) comment Blast 10th, id41%, 1e-43 Streptomyces peucetius_dnmV Thymidine diphospho-4-keto-2,3,6-trideoxyhexulose reductase [Adria_00200]NDP-hexose C-4 ketoreductase -- [PMID: 9209071](1997) dnmV: 4-keto基の還元 dnmV不活化mutant産物解析と相補による回復から、daunosamine生合成に関与することは確認されている。dTDP-4-ketodeoxyhexulose ketoreductase機能の割り当ては配列解析に基づく。 -- [PMID: 10631513](1999) 異種性ホストにおけるL-daunosamineの生合成と付着のための最少の情報は、dnmLMJVUTS genesによってコードされる。 DnmVとavrE and eryBIV genesの産物は、DNR and DXRに由来する4'-epimeric anthracyclinesの合成を通して、dTDP-4-ketohexulose reductasesであることが証明されている(ref26)。 ↓そのref26 [PMID: 9447597](1998) abstract S.peucetius dnmV mutantに、Streptomyces avermitilis avrE or Saccharopolyspora eryBIV genesを異種性に導入すると、epirubicin (4'-epidoxorubicin) and 4'-epidaunorubicinを形成。 Saccharopolyspora eryBIV = dTDP-4-keto-6-deoxy-L-hexose 4-reductase

Sequence

>putative NDP-hexose C-4 ketoreductase [putative NDP-hexose 4-keto reductase]
MPDLDKAAGGTVVVLGGTGFIGRHIGEAFTALGARVHLVSPSAVPRSGATATVRLDVLAA
SPREIAGLLATVGADTVVNSAGRVWQADEPQMAASNAELVTKVTEALAALPWQPRRPRLI
QLGSVHEYGAGTPGGAIAEDWPPAPVTPYGRTKLLGTQAVSRAVRDQDADAVVLRLANVI
GPGISVASLFGQVARHLAGAARADSLGEKPDELRLPPLRAARDLVDVRDVADAVVAAAVA
PGARVRGRVINVGRGEAVPVRDLIDRMIALSGLEVPVSEEGGAPPNRNDVEWQSLDISLA
RELLGWTPRRPLDTSLRELLAAVMPPVDERTG

>putative NDP-hexose C-4 ketoreductase [putative NDP-hexose 4-keto reductase]
TTGCCTGACCTCGACAAGGCCGCCGGCGGCACTGTCGTCGTGCTCGGCGGCACCGGGTTC
ATCGGCCGCCACATCGGTGAGGCCTTCACCGCGCTCGGGGCCCGGGTGCACCTGGTCTCC
CCCAGCGCCGTACCCCGCTCGGGCGCCACGGCCACCGTCCGCCTCGACGTGCTCGCCGCC
TCCCCCCGGGAGATCGCGGGCCTGCTCGCCACGGTCGGCGCCGACACCGTCGTCAACTCC
GCGGGGCGGGTCTGGCAGGCCGACGAGCCTCAGATGGCCGCCAGCAACGCCGAGCTGGTC
ACCAAGGTCACCGAGGCGCTCGCCGCACTCCCCTGGCAGCCGCGCAGACCCCGGCTGATC
CAGCTCGGCAGCGTCCACGAGTACGGGGCGGGCACTCCGGGAGGAGCCATCGCCGAGGAC
TGGCCGCCGGCGCCCGTCACCCCGTACGGGCGTACCAAACTCCTCGGCACCCAGGCCGTG
TCGCGGGCCGTACGGGATCAGGACGCGGACGCGGTGGTGCTCCGGCTGGCCAATGTGATC
GGCCCCGGGATCTCCGTGGCCAGTCTCTTCGGCCAGGTCGCCCGGCATCTCGCCGGGGCC
GCCCGCGCCGACTCCCTCGGCGAGAAGCCGGACGAGCTGCGGCTGCCGCCGCTGCGGGCC
GCCCGCGACCTGGTGGACGTACGGGACGTGGCGGACGCCGTGGTGGCCGCTGCCGTCGCG
CCCGGCGCGCGAGTCAGGGGGCGCGTGATCAACGTGGGCCGCGGCGAGGCCGTGCCGGTC
CGGGACCTGATCGACCGGATGATCGCGCTCAGCGGCCTCGAGGTTCCGGTGAGCGAGGAG
GGCGGCGCACCGCCGAACCGCAACGACGTCGAGTGGCAGTCCCTGGACATCTCCCTGGCG
CGCGAACTGCTCGGCTGGACGCCGCGCCGCCCGCTGGACACCTCATTGCGCGAACTGCTC
GCGGCCGTGATGCCGCCCGTCGACGAAAGGACAGGATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001509 NAD-dependent epimerase/dehydratase (Domain) PF01370 Epimerase IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 no description
SignalP	No significant hit
TMHMM	No significant hit

Sch475_00240 Sch475_00260