Sch475_00250 Sch475_00250   Sch475_00270 Sch475_00270

Sch475_00260 : CDS information

close this sectionLocation

Organism
StrainSCC-2136
Entry nameSch 47554
Contig
Start / Stop / Direction39,152 / 38,166 / - [in whole cluster]
39,152 / 38,166 / - [in contig]
Locationcomplement(38166..39152) [in whole cluster]
complement(38166..39152) [in contig]
TypeCDS
Length987 bp (328 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative dTDP-glucose 4,6-dehydratase
Product (GenBank)putative dNDP-glucose 4, 6-dehydratase
Gene
Gene (GenBank)schS5
EC number4.2.1.46
Keyword
  • L-amicetose
  • amicetose
Note
Note (GenBank)
Reference
ACC
PmId
[17085966] Angucyclines Sch 47554 and Sch 47555 from Streptomyces sp. SCC-2136: cloning, sequencing, and characterization. (Mol Cells. , 2006)
comment
angucyclines Sch 47554 and Sch 47555の生合成に関連したentire gene clusterの
クローニング、シークエンス、配列解析からの特徴づけ。

SchS5(328aa): dNDP-glucose 4, 6-dehydratase

UrdH(dNDP-glucose-4,6-dehydratase)に最も似ている。
dNDP-4-keto-6-deoxy-D-glucose形成における最初の保存された2stepsのひとつ。
Related Reference
ACC
Q56173
NITE
Grana_00110
PmId
[7476861] Identification of Streptomyces violaceoruber Tu22 genes involved in the biosynthesis of granaticin. (Mol Gen Genet. , 1995)
[9831526] The granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tu22: sequence analysis and expression in a heterologous host. (Chem Biol. , 1998)
comment
Blast 17th, id67%, 1e-122
Streptomyces violaceoruber_graE(gra-orf17)
dTDP-glucose 4,6-dehydratase (EC 4.2.1.46)

--
[PMID: 7476861](1995) abstract
ORF2はstrEに似ている。
E.coliでORF2を発現させると、dTDP-glucose→dTDP-4-keto-6-deoxy-D-glucoseへの変換を触媒することができた(controlの5倍)。
よって、ORF2はdTDP-glucose dehydrataseをコードする。

--
[PMID: 9831526](1998)
gra cluster genesのまとめ。renameあり。
ORF2→ORF17
ORF3→ORF16

close this sectionSequence

selected fasta
>putative dTDP-glucose 4,6-dehydratase [putative dNDP-glucose 4, 6-dehydratase]
MRILVTGAAGFIGSHFVRNVLEGSYSGWEDAQVTALDKLTYAGNRDNLPASHERLVFVRG
DVCDRKLLRELVPGHDAVVHFAAETHVDRSLEGAGDFFRTNVLGTQTLLDAVLDSGVERV
VHVSTDEVYGSIDEGSWTEEWPLAPNSPYAASKAGSDLVARAYWRSHGVDLSITRCSNNY
GPYQHPEKLIPLFVTNLLEGRRVPLYGEGANVREWLHVDDHCRGIHLVLNQGRAGEIYNI
GGGNERTNLAITEQLLELTGAGAEAIQRVPDRKAHDLRYSIEETKIREELGYAPRIGFEQ
GLAETVAWYRDNPDWWKAAKHGTDRAVA
selected fasta
>putative dTDP-glucose 4,6-dehydratase [putative dNDP-glucose 4, 6-dehydratase]
ATGAGGATCCTCGTCACCGGAGCGGCCGGCTTCATCGGCTCCCACTTCGTGCGCAACGTG
CTGGAGGGCTCGTACAGCGGGTGGGAGGACGCGCAGGTCACCGCCCTGGACAAGCTGACG
TACGCCGGCAACCGCGACAACCTGCCGGCCTCACACGAGCGCCTGGTGTTCGTACGCGGC
GACGTCTGCGACCGCAAGCTGCTGCGCGAGCTGGTGCCCGGCCACGACGCCGTGGTGCAC
TTCGCCGCCGAGACGCACGTCGACCGTTCCCTGGAAGGCGCGGGCGACTTCTTCCGTACG
AACGTCCTGGGCACCCAGACGCTGCTCGACGCCGTGCTCGACAGCGGCGTGGAGCGGGTC
GTGCACGTGTCCACGGACGAGGTGTACGGCTCCATCGACGAGGGCTCCTGGACGGAGGAG
TGGCCGCTCGCGCCCAACTCGCCCTACGCGGCGTCGAAGGCAGGCTCCGACCTGGTGGCC
CGCGCCTACTGGCGGAGCCACGGCGTCGACCTGTCCATCACGCGCTGCTCCAACAACTAC
GGGCCGTACCAGCACCCCGAGAAGCTCATCCCGCTGTTCGTCACGAACCTGCTCGAAGGC
CGCCGGGTCCCGTTGTACGGCGAGGGAGCCAACGTCCGTGAGTGGCTGCATGTGGACGAC
CACTGCCGGGGCATCCACCTGGTCCTGAACCAGGGCCGGGCGGGCGAGATCTACAACATC
GGCGGCGGCAACGAGCGCACCAACCTCGCCATCACCGAGCAGCTCCTCGAACTGACCGGT
GCGGGCGCCGAGGCGATCCAGCGGGTTCCCGACCGCAAGGCGCACGACCTGCGCTACTCC
ATCGAGGAGACCAAGATCCGCGAGGAGCTGGGCTACGCCCCGCGGATCGGCTTCGAACAG
GGTCTCGCCGAAACGGTCGCCTGGTACCGGGACAACCCGGACTGGTGGAAGGCCGCCAAG
CACGGAACGGACCGGGCCGTTGCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001509 NAD-dependent epimerase/dehydratase (Domain)
[3-241] 4.59999999999996e-68 PF01370
PF01370   Epimerase
IPR005888 dTDP-glucose 4,6-dehydratase (Family)
[2-318] 4.80000000000003e-126 TIGR01181
TIGR01181   DTDP_gluc_dehyt
IPR016040 NAD(P)-binding domain (Domain)
[1-188] 1.9e-65 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP No significant hit
TMHMM No significant hit
Sch475_00250 Sch475_00250   Sch475_00270 Sch475_00270
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