Sch475_00270 : CDS information

Location

Organism	Streptomyces sp.
Strain	SCC-2136
Entry name	Sch 47554
Contig	AJ628018
Start / Stop / Direction	40,216 / 39,149 / - [in whole cluster] 40,216 / 39,149 / - [in contig]
Location	complement(39149..40216) [in whole cluster] complement(39149..40216) [in contig]
Type	CDS
Length	1,068 bp (355 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	glucose-1-phosphate thymidylyltransferase dTDP-glucose synthase
Product (GenBank)	putative dNDP-glucose synthetase
Gene
Gene (GenBank)	schS6
EC number	2.7.7.24
Keyword	L-amicetose amicetose
Note
Note (GenBank)
Reference	ACC Q2HR55 PmId [17085966] Angucyclines Sch 47554 and Sch 47555 from Streptomyces sp. SCC-2136: cloning, sequencing, and characterization. (Mol Cells. , 2006) [18051285] Cloning and expression of glucose-1-phosphate thymidylyltransferase gene (schS6) from Streptomyces sp. SCC-2136. (J Microbiol Biotechnol. , 2007) comment [PMID: 17085966](2006) angucyclines Sch 47554 and Sch 47555の生合成に関連したentire gene clusterのクローニング、シークエンス、配列解析からの特徴づけ。 SchS6(355aa): dNDP-glucose synthase -- [PMID: 18051285](2007) schS6をE.coliで発現、精製、酵素活性測定。 glucose-1-phosphate thymidylyltransferase活性を示す。 dTTP + alpha-D-glucose-1-phosphate → dTDP-D-glucose + pyrophosphate

Sequence

>glucose-1-phosphate thymidylyltransferase [putative dNDP-glucose synthetase]
MKALVLAGGSGTRLRPFSYSMPKQLIPIANTPVLVHVLENIRDLGVTDIGVIVGHRGPEI
DAALGDGSRFGVKLTYISQDAPRGLAHTVAIARDFLGDDDFVMYLGDNVLPEGVAATAEE
FTARRPAAQIVVHKVTDPRQFGVAELGPDGEVLRLVEKPREPRSDMAMVGVYFFTSAIHR
AVDSIEPSARGELEITDAIQWLLASGAEVRATQYGGYWKDAGNVEDVLDCNRYLLDRLAP
SVEGDVDDLSELLGAVVVEAGARVTRSRIEGPVIIGAGAVVENSHIGPHTSIGRGCLVSD
SAVENSIALDEASVSGVKGLRSSLIGRSASVGTSEQGVDRYRLVVGDHTRVEVTA

>glucose-1-phosphate thymidylyltransferase [putative dNDP-glucose synthetase]
ATGAAGGCGCTTGTGCTGGCAGGCGGTTCCGGTACCCGCTTGCGACCCTTCAGTTATTCG
ATGCCGAAACAGCTCATCCCGATCGCCAACACCCCCGTCCTGGTACATGTTCTGGAGAAC
ATCCGGGACCTGGGCGTCACCGACATCGGCGTCATCGTCGGCCACCGGGGCCCGGAGATC
GACGCCGCCCTCGGTGACGGCTCCCGTTTCGGCGTGAAACTCACCTACATCTCCCAGGAC
GCCCCGCGCGGCCTCGCCCACACGGTGGCCATCGCCCGGGACTTCCTCGGCGACGACGAC
TTCGTGATGTACCTGGGCGACAACGTGCTGCCCGAGGGCGTCGCCGCCACCGCCGAGGAG
TTCACGGCCCGGCGTCCGGCCGCGCAGATCGTGGTCCACAAGGTGACCGACCCGCGCCAG
TTCGGTGTCGCCGAACTCGGGCCGGACGGCGAGGTGCTGCGTCTGGTGGAGAAGCCGCGC
GAACCGCGCAGCGACATGGCGATGGTCGGCGTGTACTTCTTCACCTCGGCCATCCACCGG
GCGGTGGACTCGATCGAGCCCAGCGCCCGTGGCGAGCTGGAGATCACGGACGCCATCCAG
TGGCTGCTGGCCTCCGGCGCCGAGGTCCGCGCCACCCAGTACGGCGGCTACTGGAAGGAC
GCCGGGAACGTCGAGGACGTCCTGGACTGCAACCGCTACCTCCTGGACCGGCTGGCGCCG
TCCGTCGAGGGTGACGTCGACGACCTCAGCGAGCTGCTGGGCGCGGTCGTCGTCGAGGCG
GGGGCGCGCGTGACACGGTCGCGCATCGAGGGGCCGGTGATCATCGGAGCGGGCGCGGTG
GTGGAGAACAGCCACATCGGCCCGCACACGTCCATCGGGCGCGGCTGCCTGGTCAGCGAC
AGCGCCGTGGAGAACTCCATCGCCCTCGACGAGGCCTCGGTCAGCGGCGTCAAGGGCCTG
CGCAGTTCGCTGATCGGGCGGTCCGCCTCGGTCGGCACCAGTGAGCAGGGCGTCGACCGG
TACCGGCTGGTCGTCGGAGACCACACCCGAGTGGAGGTCACGGCATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005835 Nucleotidyl transferase (Domain) PF00483 NTP_transferase IPR005908 Glucose-1-phosphate thymidylyltransferase, long form (Family) TIGR01208 RmlA_long
SignalP	No significant hit
TMHMM	No significant hit

Sch475_00260 Sch475_00280