Sch475_00280 Sch475_00280   Sch475_00300 Sch475_00300

Sch475_00290 : CDS information

close this sectionLocation

Organism
StrainSCC-2136
Entry nameSch 47554
Contig
Start / Stop / Direction42,039 / 41,434 / - [in whole cluster]
42,039 / 41,434 / - [in contig]
Locationcomplement(41434..42039) [in whole cluster]
complement(41434..42039) [in contig]
TypeCDS
Length606 bp (201 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative NDP-hexose epimerase
Product (GenBank)putative NDP-hexose 3, 5-epimerase
Gene
Gene (GenBank)schS8
EC number5.1.3.-
Keyword
  • L-amicetose
  • L-aculose
Note
  • The N-terminal position was modified from original INSDC entry.
Note (GenBank)
Reference
ACC
PmId
[17085966] Angucyclines Sch 47554 and Sch 47555 from Streptomyces sp. SCC-2136: cloning, sequencing, and characterization. (Mol Cells. , 2006)
comment
angucyclines Sch 47554 and Sch 47555の生合成に関連したentire gene clusterの
クローニング、シークエンス、配列解析からの特徴づけ。

SchS8(196aa): NDP-hexose 3,5-epimerase

L-rhodinose生合成に関連したUrdZ1(NDP-glucose 3,5-epimerase)に似ている。
Related Reference
ACC
Q331R1
PmId
[15049360] Cloning, expression, and biological function of a dTDP-deoxyglucose epimerase (gerF) gene from Streptomyces sp. GERI-155. (Biotechnol Lett. , 2004)
[17053005] Biosynthesis of dTDP-6-deoxy-beta-D-allose, biochemical characterization of dTDP-4-keto-6-deoxyglucose reductase (GerKI) from Streptomyces sp. KCTC 0041BP. (Glycobiology. , 2007)
comment
Blast 56th, id42%, 1e-37
Streptomyces sp. KCTC 0041BP_gerF
NDP-hexose-3-epimerase

---
[PMID: 15049360](2004)
Streptomyces sp. GERI-155 = Streptomyces sp. KCTC 0041BP

dTDP-deoxyglucose epimerase geneをコードすると思われるgerFをE.coliで発現、精製、機能確認。
精製された酵素はdTDP-4-keto-6-deoxyglucoseからmaltolを産生し、よって発現されたタンパクがdTDP-4-keto-6-deoxyglucoseのC-3 and C-5 or C-3の異性化を触媒するdTDP-deoxyglucose epimeraseであることが確認された。

---
[PMID: 17053005](2007)
gerK1をクローニング、E.coliで過剰発現、機能確認。
GerK1はC-3でaxialなOH基を持つときのみ、dTDP-4-keto-6-deoxy-D-alloseの4-keto基で特異的な還元効果を示す。
GerFの存在下で、GerK1はdTDP-4-keto-6-deoxyglucose → dTDP-6-deoxy-beta-D-alloseへの変換を触媒した。
このdTDP-6-deoxy-D-alloseの分離成功は、GerFがmycinose生合成経路において3,5-epimeraseではなく、3-epimeraseとして機能することを確認するのに十分な証拠を供給する。
ACC
Q9S0P2
NITE
Aver_00150
PmId
[11451669] Insights about the biosynthesis of the avermectin deoxysugar L-oleandrose through heterologous expression of Streptomyces avermitilis deoxysugar genes in Streptomyces lividans. (Chem Biol. , 2001)
comment
Blast 96th, id43%, 4e-35
Streptomyces avermitilis_aveBV(avrF)
dTDP-4-keto-6-deoxyhexose 3,5-epimerase
[Aver_00150]dTDP-4-keto-6-deoxyhexose 5-epimerase

Table1.
avrF/aveBV: dTDP-4-keto-6-deoxyglucose 3-epimerase　
(本文を読むと5-epimeraseの間違いと思われる。)

S.lividansでS.avermitilis avrBCDEFGHIを発現させて外因性aglyconeと反応させて産物解析。
delta-avrFな発現株では、C-13にD-olivoseが1つbeta-glycoside結合したavermectin A1a3を産生する。
avermectin A1a3を産生できるのはdelta-avrFのみ。
C-3での立体化学は最終的に3-O-methyltransferaseによって決定されるので、AvrF proteinは5-epimeraseであって3,5-epimeraseではない。

close this sectionSequence

selected fasta
>putative NDP-hexose epimerase [putative NDP-hexose 3, 5-epimerase]
MLIEEMVIPGTYLVTPRQIHDERGNFYEAMRDDTLEKATGVAFQPRQINYSVSRRHTLRG
IHSVSIPPGQAKYVTCVRGVLRDIVVDLRIGSPAFMKHQVNELDADSGRSVYVPEGVGHG
FLALTDDACICYVVSSTYVPGTQIDINPLDPELDLPWDCPEVPLISDKDAKAPTVAEARA
AGVLPRFGGTGTPRFTKAGTL
selected fasta
>putative NDP-hexose epimerase [putative NDP-hexose 3, 5-epimerase]
GTGCTGATCGAGGAAATGGTCATTCCCGGTACATATCTGGTGACGCCCCGTCAGATACAC
GATGAGCGGGGGAACTTCTACGAGGCGATGCGTGACGACACGCTGGAGAAGGCGACCGGT
GTCGCCTTCCAGCCCCGCCAGATCAACTACTCGGTGTCGAGACGCCATACGCTGCGCGGC
ATCCACAGCGTGAGCATTCCGCCGGGTCAGGCGAAGTACGTCACCTGTGTGCGGGGCGTG
CTGCGCGACATCGTGGTCGATCTGCGGATCGGTTCCCCGGCGTTCATGAAGCACCAGGTC
AACGAACTCGACGCGGATTCGGGCAGATCCGTCTATGTGCCCGAGGGCGTCGGCCACGGC
TTTCTCGCGCTCACCGACGACGCCTGCATCTGCTACGTCGTCTCCAGCACCTATGTGCCC
GGAACGCAGATCGACATCAATCCACTCGACCCCGAGCTCGATCTGCCGTGGGACTGCCCC
GAAGTCCCCCTCATCTCCGACAAGGACGCCAAGGCACCGACCGTGGCCGAGGCCAGAGCC
GCCGGCGTTCTGCCCCGGTTCGGCGGGACAGGCACGCCCCGATTCACCAAGGCAGGAACA
CTGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000888 dTDP-4-dehydrorhamnose 3,5-epimerase-related (Family)
[4-177] 6.5e-48 PF00908
PF00908   dTDP_sugar_isom
[8-182] 5e-65 PD001462
PD001462   Q9RPA8_STRFR_Q9RPA8;
IPR011051 RmlC-like cupin domain (Domain)
[1-182] 1.9e-65 SSF51182
SSF51182   RmlC-like cupins
IPR014710 RmlC-like jelly roll fold (Domain)
[1-186] 4.2e-56 G3DSA:2.60.120.10
G3DSA:2.60.120.10   no description
SignalP No significant hit
TMHMM No significant hit
Sch475_00280 Sch475_00280   Sch475_00300 Sch475_00300
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