Sch475_00300 Sch475_00300   Sch475_00320 Sch475_00320

Sch475_00310 : CDS information

close this sectionLocation

Organism
StrainSCC-2136
Entry nameSch 47554
Contig
Start / Stop / Direction44,590 / 43,298 / - [in whole cluster]
44,590 / 43,298 / - [in contig]
Locationcomplement(43298..44590) [in whole cluster]
complement(43298..44590) [in contig]
TypeCDS
Length1,293 bp (430 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative glycosyltransferase
Product (GenBank)putative O-glycosyltransferase
Gene
Gene (GenBank)schS10
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[17085966] Angucyclines Sch 47554 and Sch 47555 from Streptomyces sp. SCC-2136: cloning, sequencing, and characterization. (Mol Cells. , 2006)
comment
angucyclines Sch 47554 and Sch 47555の生合成に関連したentire gene clusterの
クローニング、シークエンス、配列解析からの特徴づけ。

SchS10(430aa): O-glycosyltransferase

UrdGT1aに似ていることから、Sch 47554 and Sch 47555においてC-3 and C-9両方にL-aclosylを移動するかもしれないが、SchS9 and SchS10の割り当てはさらなる実験での検証を必要とする。glycosylateの順番も解明されていない。
Related Reference
ACC
C4NYK9
PmId
comment
Blast 2nd, id54%, 1e-113
Micromonospora sp. Tu 6368_saqGT2
SaqGT2

abstract

Micromonospora sp. Tu6368で産生されるsaquayamycin Z and galtamycin Bは同じ生合成gene clusterに由来する。不活化実験により5つあるglycosyltransferasesの機能を解明したとのこと。
ACC
Q9RPA2
NITE
Urd_00030
PmId
[10662691] Function of glycosyltransferase genes involved in urdamycin A biosynthesis. (Chem Biol. , 2000)
[11094336] The NDP-sugar co-substrate concentration and the enzyme expression level influence the substrate specificity of glycosyltransferases: cloning and characterization of deoxysugar biosynthetic genes of the urdamycin biosynthetic gene cluster. (Chem Biol. , 2000)
comment
Blast 6th id49%, 1e-101
Streptomyces fradiae_urdGT1a
Glycosyl transferase
[Urd_00030]L-rhodinose glycosyltransferase

--
[PMID: 10662691](2000)
[PMID: 11094336](2000)

urdamycin clusterに含まれるglycosyltransferase候補genes=urdGT1a, urdGT1b, urdGT1cについて、mutant作成、相補実験における蓄積産物解析を行い、各機能割り当てが確定されている。

UrdGT1aは、urdamycin A生合成においてC-12bのhydroxy基にL-rhodinoseを付着するO-glycosyltransferaseである。

C-9にあるtrisaccharideでもL-rhodinoseを付着するglycosyltransferase(UrdGT1c)が作用するが、UrdGT1aはこれを代替しない。

close this sectionSequence

selected fasta
>putative glycosyltransferase [putative O-glycosyltransferase]
MRVLLTIFPATSHLYSVVPLAWALQSAGHEVVVASHSGVVDPGVIGNIGAAGLTAVSLGT
PDELSPALGAHTGDAKPDRPSLGFDPNEPEESGGWRTARSILASMFSLYYPAPERQGGRR
PVLDNLVDFARAWRPDLVLWDPLMLAAPIAARVSGAAHARLVWGMDNVAVIHDRTKRELA
DPRSELTEHPWLSWFGPMLERYGLELDDEMLLGQWTLDLTQSRMRHPLDLTHIPVRRVPY
TGAAALPDWLHARPERPRAVLTLGVSRRKIFGKYSGFPMREFFESVSALDVEVVATLNNE
QLAAVGKVPDNVRTVEYVPLNQVLPTSSAIIHHGGGGTFAAAVAHRVPQLVVPLVMWDEM
VTARYVADMGAGLVADPAALDVDGLHKQLVRLLEDPSFQLGARGLYEEMLAAPAPTDVVP
LLERLTAERR
selected fasta
>putative glycosyltransferase [putative O-glycosyltransferase]
ATGCGTGTCCTGCTCACCATCTTTCCCGCGACGTCACACCTGTACTCGGTCGTGCCGCTT
GCCTGGGCGCTGCAGAGCGCCGGGCACGAGGTCGTCGTGGCGAGCCACTCGGGCGTGGTG
GACCCGGGGGTGATCGGGAACATCGGCGCGGCCGGGCTCACCGCGGTGTCGCTCGGCACC
CCGGACGAGCTGTCCCCGGCGCTCGGTGCGCACACCGGGGACGCCAAGCCGGACCGTCCG
TCGCTGGGCTTCGACCCCAACGAGCCGGAGGAGAGCGGCGGCTGGCGCACGGCGCGCTCC
ATCCTCGCCAGCATGTTCTCGCTGTACTACCCGGCGCCCGAGCGCCAGGGCGGACGCCGC
CCGGTCCTGGACAACCTGGTCGACTTCGCCCGCGCCTGGCGCCCCGATCTGGTCCTGTGG
GACCCCCTGATGCTCGCGGCCCCGATCGCCGCGCGGGTCAGCGGCGCCGCGCACGCCCGG
CTGGTCTGGGGCATGGACAACGTCGCCGTGATCCACGACCGCACGAAGCGCGAACTGGCG
GACCCGCGCTCGGAGTTGACCGAGCATCCGTGGCTGAGCTGGTTCGGCCCGATGCTGGAG
CGGTACGGGCTGGAGCTCGACGACGAGATGCTGCTCGGCCAGTGGACCCTCGACCTGACC
CAGTCCCGGATGCGGCATCCCCTGGACCTGACACACATCCCGGTCCGCCGGGTCCCCTAC
ACCGGGGCGGCCGCACTGCCCGACTGGCTGCACGCGCGGCCCGAGCGGCCCCGCGCGGTG
CTGACCCTGGGGGTGAGCCGGCGCAAGATCTTCGGCAAGTACAGCGGCTTCCCGATGCGC
GAGTTCTTCGAATCCGTGTCCGCCCTGGACGTCGAGGTCGTCGCCACCCTCAACAACGAG
CAGCTCGCGGCGGTCGGCAAGGTGCCCGACAACGTGCGCACCGTCGAGTACGTGCCGCTC
AACCAGGTTCTGCCGACCAGTTCGGCGATCATCCACCACGGAGGCGGCGGCACCTTCGCC
GCGGCCGTGGCCCACCGGGTGCCGCAGCTCGTGGTGCCGCTGGTCATGTGGGACGAGATG
GTCACGGCGCGCTATGTCGCCGACATGGGCGCGGGCCTGGTCGCCGACCCCGCCGCGCTG
GACGTCGACGGCCTGCACAAGCAGCTCGTACGACTCCTCGAGGACCCGTCGTTCCAGCTC
GGCGCGCGCGGCCTGTACGAGGAGATGCTCGCCGCGCCGGCGCCCACGGACGTCGTACCG
CTGCTGGAACGTCTGACCGCCGAGCGCCGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR004276 Glycosyl transferase, family 28 (Domain)
[3-92] 2.7e-07 PF03033
PF03033   Glyco_transf_28
IPR010610 Domain of unknown function DUF1205 (Domain)
[218-313] 9.40000000000003e-27 PF06722
PF06722   DUF1205
SignalP
[1-23] 0.949 Signal
Eukaryota   
[1-23] 0.477 Signal
Bacteria, Gram-positive   
[1-23] 0.566 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Sch475_00300 Sch475_00300   Sch475_00320 Sch475_00320
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