Sch475_00350 Sch475_00350   Sch475_00370 Sch475_00370

Sch475_00360 : CDS information

close this sectionLocation

Organism
StrainSCC-2136
Entry nameSch 47554
Contig
Start / Stop / Direction50,037 / 49,768 / - [in whole cluster]
50,037 / 49,768 / - [in contig]
Locationcomplement(49768..50037) [in whole cluster]
complement(49768..50037) [in contig]
TypeCDS
Length270 bp (89 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase acyl carrier protein subunit
Product (GenBank)putative acyl carrier protein
Gene
Gene (GenBank)schP6
EC number
Keyword
  • type II PKS
Note
Note (GenBank)
Reference
ACC
PmId
[17085966] Angucyclines Sch 47554 and Sch 47555 from Streptomyces sp. SCC-2136: cloning, sequencing, and characterization. (Mol Cells. , 2006)
comment
angucyclines Sch 47554 and Sch 47555の生合成に関連したentire gene clusterの
クローニング、シークエンス、配列解析からの特徴づけ。

SchP6(89aa): Acyl carrier protein
Related Reference
ACC
Q54219
NITE
Griseu_00030
PmId
[8169211] Cloning, sequencing, and analysis of the griseusin polyketide synthase gene cluster from Streptomyces griseus. (J Bacteriol. , 1994)
[9108302] Post-translational modification of heterologously expressed Streptomyces type II polyketide synthase acyl carrier proteins. (FEBS Lett. , 1997)
[9738947] Acylation of Streptomyces type II polyketide synthase acyl carrier proteins. (FEBS Lett. , 1998)
comment
Blast 3rd, id73%, 8e-27
Streptomyces griseus_ORF3
Acyl carrier protein

--
[PMID: 8169211](1994)
griseusin polyketide synthase gene clusterの報告。

このORF3を含んだ領域の置換で、griseusins A and B産生能力を損失。
ORF3単独の機能は、配列解析から述べられている。

--
[PMID: 9108302](1997)
E.coliにおけるactinorhodin (act), oxytetracycline (otc) and griseusin (gris) apo-ACPsのholo型形成に関する調査。

--
[PMID: 9738947](1998)
上記論文の続報。
holo-ACPsによるacylationの調査。

close this sectionPKS/NRPS Module

ACP7..80

close this sectionSequence

selected fasta
>polyketide synthase acyl carrier protein subunit [putative acyl carrier protein]
MPAHEFTIEDLRRILRDGAGTDEAVDLDGDILDTDFESLGYESLALLETSGRIEREFGIT
LDDDTVTEAKTPRALIGAVNTFLVPVRSS
selected fasta
>polyketide synthase acyl carrier protein subunit [putative acyl carrier protein]
ATGCCCGCTCACGAGTTCACCATCGAAGACCTTCGGCGCATTCTGCGCGACGGGGCCGGC
ACCGATGAGGCCGTCGACCTGGACGGCGACATCCTCGACACGGACTTCGAGTCTCTCGGA
TACGAGTCCCTGGCCCTGCTGGAGACCAGCGGCCGGATCGAGCGCGAGTTCGGGATCACG
CTCGACGACGACACGGTGACGGAGGCGAAGACACCTCGCGCGCTGATCGGCGCCGTCAAC
ACCTTCCTGGTGCCCGTCCGGTCCAGCTGA
ACP7..80
ACP19..240

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR006162 Phosphopantetheine attachment site (PTM)
[38-53] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[11-78] 6.00000000000001e-10 PF00550
PF00550   PP-binding
[5-80] 6.60000000000002e-16 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[7-80] PS50075
PS50075   ACP_DOMAIN
[3-83] 4.30000170645867e-14 SSF47336
SSF47336   ACP_like
SignalP
[1-24] 0.035 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Sch475_00350 Sch475_00350   Sch475_00370 Sch475_00370
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