Sch475_00340 Sch475_00340   Sch475_00360 Sch475_00360

Sch475_00350 : CDS information

close this sectionLocation

Organism
StrainSCC-2136
Entry nameSch 47554
Contig
Start / Stop / Direction49,735 / 48,935 / - [in whole cluster]
49,735 / 48,935 / - [in contig]
Locationcomplement(48935..49735) [in whole cluster]
complement(48935..49735) [in contig]
TypeCDS
Length801 bp (266 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative polyketide C-9 ketoreductase
Product (GenBank)putative ketoreducatse
Gene
Gene (GenBank)schP5
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[17085966] Angucyclines Sch 47554 and Sch 47555 from Streptomyces sp. SCC-2136: cloning, sequencing, and characterization. (Mol Cells. , 2006)
comment
angucyclines Sch 47554 and Sch 47555の生合成に関連したentire gene clusterの
クローニング、シークエンス、配列解析からの特徴づけ。

SchP5(266aa): Ketoreductase

schP4-schP5のC末をtsrで置換して不活化したplasmidをStreptomyces sp. SCC-2136に移入。
このmutantがSch47554もSch47555も産生していないことを確認。
Related Reference
ACC
Q56166
NITE
Jado_00110
PmId
[7881555] Cloning and characterization of polyketide synthase genes for jadomycin B biosynthesis in Streptomyces venezuelae ISP5230. (Microbiology. , 1994)
[9224566] Iterative type II polyketide synthases, cyclases and ketoreductases exhibit context-dependent behavior in the biosynthesis of linear and angular decapolyketides. (Chem Biol. , 1997)
comment
Blast 4th, id82%, 1e-117
Streptomyces venezuelae_ORF5(jadE)
Ketoreductase

--
[PMID: 7881555](1994)
ORF5(=jadEに相当)は配列解析からketoreductaseであると提唱されている。
ORF2-ORF4領域(ORF5も含まれる)を含んだplasmidの染色体DNAへの組み込みは、jadomycin B産生を激しく抑制したが、成長や胞子色素沈着には影響なし。

--
[PMID: 9224566](1997)
minimal PKS + KR + CYCをjadomycin, daunorubicin, tetracenomycinのgenesで構成し、
そのplasmid発現株での産物を解析。
JadEはdecaketideだけでなく、octaketideも還元できる。

close this sectionSequence

selected fasta
>putative polyketide C-9 ketoreductase [putative ketoreducatse]
MTTATSGNTEKKVAVVTGATSGIGLAVTRLLAEQGHRVFLCARTEDGVTRTVKELLDEGL
DVDGAVCDVREGEDVKRFVRQAVERFGPIDVLVNNAGRSGGGPTAGIADELWYDVIATNL
HSVFLMSREVLNTGGMAAKERGRIINIASTAGKQGVVLGAPYSASKHGVVGFTKALGNEL
APTGITVNAVCPGYVETPMAQRVRQGYAAAYDTSEDAILQKFQAKIPLGRYSTPDEVAGL
VGYLASDTAASITSQALNVCGGLGNF
selected fasta
>putative polyketide C-9 ketoreductase [putative ketoreducatse]
ATGACGACTGCCACGTCCGGCAACACGGAGAAGAAGGTCGCCGTCGTCACCGGCGCGACC
AGCGGTATCGGCCTCGCGGTGACCCGGCTCCTCGCCGAGCAGGGTCACCGGGTGTTCCTG
TGCGCCCGCACCGAGGACGGTGTCACCCGCACGGTCAAGGAGCTGCTCGACGAGGGCCTC
GACGTCGACGGCGCCGTCTGCGACGTACGCGAGGGCGAGGACGTGAAGCGCTTCGTGCGG
CAGGCCGTCGAGCGCTTCGGCCCCATCGACGTCCTGGTCAACAACGCGGGCCGCAGCGGC
GGCGGTCCCACCGCCGGCATCGCCGACGAACTCTGGTACGACGTCATCGCCACGAACCTG
CACAGCGTGTTCCTGATGAGCCGCGAGGTGCTCAACACGGGCGGCATGGCGGCCAAGGAG
CGCGGCCGGATCATCAACATCGCCTCCACGGCAGGAAAGCAGGGTGTCGTGCTCGGCGCC
CCCTACTCGGCCTCCAAGCACGGCGTCGTCGGCTTCACCAAAGCCCTCGGCAACGAACTG
GCCCCGACCGGCATCACCGTCAACGCCGTGTGCCCCGGCTATGTCGAGACGCCGATGGCC
CAGCGCGTACGGCAGGGCTATGCGGCCGCGTACGACACCTCCGAGGACGCGATCCTGCAG
AAGTTCCAGGCGAAGATCCCGCTCGGCCGCTACTCCACGCCCGACGAGGTGGCCGGCCTG
GTCGGCTACCTCGCCTCGGACACCGCCGCCTCCATCACCTCGCAGGCCCTGAACGTCTGC
GGCGGCCTCGGCAATTTCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
[87-98] 1.20000117458134e-14 PR00080 [142-150] 1.20000117458134e-14 PR00080 [162-181] 1.20000117458134e-14 PR00080
PR00080   SDRFAMILY
[12-180] 1.40000000000001e-36 PF00106
PF00106   adh_short
[1-253] 6.69999687625999e-17 PIRSF000126
PIRSF000126   11-beta-HSD1
IPR002347 Glucose/ribitol dehydrogenase (Family)
[13-30] 1.40000506907309e-49 PR00081 [87-98] 1.40000506907309e-49 PR00081 [136-152] 1.40000506907309e-49 PR00081 [162-181] 1.40000506907309e-49 PR00081 [183-200] 1.40000506907309e-49 PR00081 [227-247] 1.40000506907309e-49 PR00081
PR00081   GDHRDH
IPR016040 NAD(P)-binding domain (Domain)
[5-263] 4.59999999999996e-83 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR020904 Short-chain dehydrogenase/reductase, conserved site (Conserved_site)
[149-177] PS00061
PS00061   ADH_SHORT
SignalP
[1-32] 0.652 Signal
Bacteria, Gram-positive   
TMHMM
[13-32] Transmembrane (i-o)
Transmembrane 1   
Sch475_00340 Sch475_00340   Sch475_00360 Sch475_00360
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