Sch475_00450 Sch475_00450   Sch475_00470 Sch475_00470

Sch475_00460 : CDS information

close this sectionLocation

Organism
StrainSCC-2136
Entry nameSch 47554
Contig
Start / Stop / Direction60,257 / 61,093 / + [in whole cluster]
60,257 / 61,093 / + [in contig]
Location60257..61093 [in whole cluster]
60257..61093 [in contig]
TypeCDS
Length837 bp (278 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Productputative transcriptional regulator
Product (GenBank)putative repressor-response regulator
Gene
Gene (GenBank)schA25
EC number
Keyword
Note
  • The N-terminal position was modified from original INSDC entry.
Note (GenBank)
Reference
ACC
PmId
[17085966] Angucyclines Sch 47554 and Sch 47555 from Streptomyces sp. SCC-2136: cloning, sequencing, and characterization. (Mol Cells. , 2006)
comment
angucyclines Sch 47554 and Sch 47555の生合成に関連したentire gene clusterの
クローニング、シークエンス、配列解析からの特徴づけ。

SchA25(283aa): Repressor-response regulator

landomycin A pathwayでpositive regulationを担うLanI に似ている。
Related Reference
ACC
Q66PH7
PmId
[12757959] Production of landomycins in Streptomyces globisporus 1912 and S cyanogenus S136 is regulated by genes encoding putative transcriptional activators. (FEMS Microbiol Lett. , 2003)
[17786405] Function of lanI in regulation of landomycin A biosynthesis in Streptomyces cyanogenus S136 and cross-complementation studies with Streptomyces antibiotic regulatory proteins encoding genes. (Arch Microbiol. , 2008)
comment
Blast 3rd, id67%, 1e-66
Streptomyces globisporus
LndI

---
[PMID: 12757959](2003)
regulatory genes
lanI がlandomycin A (LaA) producer Streptomyces cyanogenus S136から、
lndI がlandomycin E (LaE) producer Streptomyces globisporus 1912から、
クローニングされ、シークエンスされた。

破壊されたlndI geneを持つS. globisporus I2-1の培養は、landomycin Eやその他関連中間体を産生しなかった。lndI or lanI で補完され、landomycin E産生を回復。

よって、LanI and LndI はそれぞれのclustersにおいて構造遺伝子の活性化に関連することが示された。

---
[PMID: 17786405](2008)
Streptomyces cyanogenus S136のlanI を不活化。
得られたmutant株はlandomycin Aや知られた中間体を産生しなかった。
RT-PCR解析でいくらかのlan genesの転写の完全な休止が確認され、それにはlanJとlanKが含まれた。
mutant株へのlanI or lndI の導入でlandomycin A産生を回復。
ACC
Q56HA6
PmId
[16079347] Characterization of a regulatory gene essential for the production of the angucycline-like polyketide antibiotic auricin in Streptomyces aureofaciens CCM 3239. (Microbiology. , 2005)
[20466770] The role of the TetR-family transcriptional regulator Aur1R in negative regulation of the auricin gene cluster in Streptomyces aureofaciens CCM 3239. (Microbiology. , 2010)
[21584781] Genetic manipulation of pathway regulation for overproduction of angucycline-like antibiotic auricin in Streptomyces aureofaciens CCM 3239. (Folia Microbiol (Praha). , 2011)
comment
Blast 10th, id60%, 1e-57
Streptomyces aureofaciens_aur1P
Aur1P
[Aur_00450]transcriptional activator

--
[PMID: 16079347](2005)
aur1P, aur1Rの同定。aur1Pの特徴づけ。

aur1Pの発現はsingle promoter aur1Ppによって方向づけられ、対数期は低レベルで転写され、静止期の直前に誘導された。
aur1P破壊mutantは生育と分化に影響しないがauricin非産生、promoter aur1Apに由来する発現は激減。

Aur1Pはaur1Ap promoter領域の上流に特異的に結合。
よってAur1P regulatorがauricin生合成遺伝子の発現を活性化する。

--
[PMID: 20466770](2010)
aur1Rの特徴づけ。
Aur1Rはaur1Pp promoterへ結合し、aur1Pの発現を特異的に抑制する。

--
[PMID: 21584781](2011) abstract
aur1PをermEp* promoter下に置くと、auricin産生量がwildの2.8倍に増加。

close this sectionSequence

selected fasta
>putative transcriptional regulator [putative repressor-response regulator]
MSNQSTKFLEPAIPQQLANRRAPTPSRPVSQHAQQHPPIQAQQLPPIQAQPQLRKARGVL
RVLVVESGAPAAQSLVLSLARHGYQAVSVDTGSKALQKYREADVLLIDLDLPDLDGIEIC
RFVRAASGVGVIVVTARDTELDRVLSLKAGADDYVVKPYSLPELMARIEAVMRRVRPLPL
TTEVISSGPLRIDSRTREIRLGDEPVEVTRKEFDLLYLLASRPGSVVSRKQIMTLVWGDS
RSQPGRTIDTHVSSLRSKLGSSSWIITARGVGFRFGHA
selected fasta
>putative transcriptional regulator [putative repressor-response regulator]
ATGAGCAATCAGTCCACAAAATTTCTCGAGCCCGCCATTCCGCAACAACTGGCCAATCGC
CGCGCGCCAACGCCGTCGCGCCCAGTGAGCCAACATGCCCAGCAGCATCCGCCCATACAG
GCCCAGCAACTACCGCCCATCCAGGCCCAGCCTCAGCTGCGCAAGGCGCGCGGGGTGTTG
CGTGTCCTCGTTGTCGAGAGCGGGGCGCCCGCCGCCCAGTCCCTGGTTCTGAGTCTGGCC
AGGCACGGGTACCAGGCCGTGAGCGTGGACACCGGCAGTAAGGCGCTCCAGAAGTACCGG
GAAGCGGATGTCCTGCTCATCGACCTGGACCTGCCCGACCTGGACGGCATCGAGATCTGC
CGTTTCGTCAGGGCCGCGTCCGGGGTCGGAGTCATCGTTGTCACGGCCCGCGACACGGAA
CTCGACCGCGTGCTCAGCCTCAAGGCGGGCGCGGACGACTATGTGGTGAAGCCCTACAGT
CTGCCCGAGCTGATGGCCCGTATCGAAGCCGTCATGCGACGGGTGCGCCCGCTGCCACTG
ACCACCGAAGTGATCAGCAGCGGCCCGTTGCGGATCGACTCGCGCACCCGTGAGATCCGG
CTCGGCGACGAGCCCGTCGAAGTGACGCGCAAGGAGTTCGACCTGCTTTATCTGCTGGCC
TCGCGGCCGGGCAGCGTCGTTTCCCGAAAGCAAATCATGACACTGGTCTGGGGCGATTCC
CGCTCCCAGCCGGGTCGCACGATCGACACGCATGTCAGTAGTCTCCGCAGTAAACTCGGA
TCCAGCAGCTGGATCATCACGGCCCGCGGTGTCGGTTTCCGATTCGGTCATGCGTAA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001789 Signal transduction response regulator, receiver domain (Domain)
[60-168] 1.6e-29 SM00448
SM00448   cheY-homologous receiver domain
[61-172] 31.613 PS50110
PS50110   RESPONSE_REGULATORY
[62-168] 6.6e-19 PF00072
PF00072   Response_reg
IPR001867 Signal transduction response regulator, C-terminal (Domain)
[203-275] 1.1e-22 SM00862
SM00862   Transcriptional regulatory protein, C ter
[204-275] 4.2e-21 PF00486
PF00486   Trans_reg_C
IPR011006 CheY-like superfamily (Domain)
[60-245] 1.8e-30 SSF52172
SSF52172   CheY-like
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
[182-275] 9.9e-27 G3DSA:1.10.10.10
G3DSA:1.10.10.10   no description
SignalP No significant hit
TMHMM No significant hit
Sch475_00450 Sch475_00450   Sch475_00470 Sch475_00470
Page top