Sch475_00460 Sch475_00460   Sch475_00480 Sch475_00480

Sch475_00470 : CDS information

close this sectionLocation

Organism
StrainSCC-2136
Entry nameSch 47554
Contig
Start / Stop / Direction63,931 / 62,333 / - [in whole cluster]
63,931 / 62,333 / - [in contig]
Locationcomplement(62333..63931) [in whole cluster]
complement(62333..63931) [in contig]
TypeCDS
Length1,599 bp (532 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative FAD-dependent oxidoreductase
Product (GenBank)putative oxidoreducatse
Gene
Gene (GenBank)schA26
EC number
Keyword
  • L-aculose
Note
Note (GenBank)
Reference
ACC
PmId
[17085966] Angucyclines Sch 47554 and Sch 47555 from Streptomyces sp. SCC-2136: cloning, sequencing, and characterization. (Mol Cells. , 2006)
comment
angucyclines Sch 47554 and Sch 47555の生合成に関連したentire gene clusterの
クローニング、シークエンス、配列解析からの特徴づけ。

SchA26(532aa): Oxidoreductase

本文コメントなし。
Related Reference
ACC
Q0PCD7
NITE
Acla_00150
PmId
[17242508] Structure determination by multiwavelength anomalous diffraction of aclacinomycin oxidoreductase: indications of multidomain pseudomerohedral twinning. (Acta Crystallogr D Biol Crystallogr. , 2007)
[17395717] Aclacinomycin oxidoreductase (AknOx) from the biosynthetic pathway of the antibiotic aclacinomycin is an unusual flavoenzyme with a dual active site. (Proc Natl Acad Sci U S A. , 2007)
comment
Blast 2nd, id53%, 1e-147
Streptomyces galilaeus_aknOx
Aclacinomycin oxidoreductase

[PMID: 17242508](2007) abstract
[PMID: 17395717](2007)

Aclacinomycin oxidoreductase (AknOx)は、Aclacinomycinグループのpolyketide抗生物質における最後の2steps、末端糖部分rhodinose→L-aculoseへの酸化を触媒する。

3つめの糖が
rhodinose (Aclacinomycin N)
↓1st: C-4 hydroxy基からプロトン除去[Tyr-450]
cinerulose A (Aclacinomycin A)
↓2nd: cineruloseのC3からプロトン除去[Tyr-378]
L-aculose (Aclacinomycin Y)

AknOxの異なる２つの反応が同じactive siteで触媒される。
この反応はFADの還元を伴う。

close this sectionSequence

selected fasta
>putative FAD-dependent oxidoreductase [putative oxidoreducatse]
MVSRRTFLGTSIVVAGTTTGLSAVAPAAHAGTTAAAAVPPEVIVRPGDTRYAELAGRGYN
TRFRAAPDEIWLVHNASQVEKAVTQAIRKDQRITVRSGGHCFDGLVDDPQFRLLVDTSEM
KRVYFDSSMNAFAVESGARLGEVYKALYEGWGVTLPGGVCPEVGVGGHVSGGGYGPLSRR
YGLSVDHLYAVEVVVAGSDGKARTIVATSRRSDPNRDLWWAHTGGGGGQFGIVTRFWFRT
PGASGAPTALLPKPPARMRKSLVTWQWSDITEAGFTRLLLNHGAWHAANSGPDSPYATMH
SSFQLRSSLVGSFVLEIRMDATLAATPKLFDDYLAAVSAGVGVTPAVDTREMPWVEAATE
PTPPFGTYSRHKSIGGHLRKPLTAAQVGVVYRSLTDPEHSTGAGLVYLAAYGCKINTVSS
SATAVAQRDSIFKVWYSTNWSEPSADAAEVEWIRTLRKGVHSATGGFPVPNDQQDGGYVN
YPDVDIRDPYQNSSGVPWYTILYKDNHPRLQRVKNTYDPRNVFRHGLSLNPS
selected fasta
>putative FAD-dependent oxidoreductase [putative oxidoreducatse]
ATGGTTTCGCGCCGCACGTTCCTTGGCACCTCGATAGTTGTGGCCGGTACCACGACGGGC
CTGTCCGCCGTGGCACCGGCCGCCCACGCCGGCACGACCGCCGCCGCGGCCGTTCCGCCC
GAGGTGATCGTCAGACCCGGCGACACGCGCTACGCCGAGCTGGCGGGGCGCGGCTACAAC
ACCCGCTTCCGAGCCGCCCCGGACGAGATCTGGCTGGTGCACAACGCCTCCCAGGTGGAG
AAGGCGGTCACCCAGGCGATCCGCAAGGACCAGCGGATCACCGTACGCAGCGGCGGCCAC
TGCTTCGACGGCCTGGTTGACGACCCCCAGTTCCGTCTCCTCGTCGACACCTCGGAGATG
AAGCGGGTCTACTTCGACAGCTCGATGAACGCCTTCGCCGTCGAGTCCGGCGCCCGGCTC
GGCGAGGTCTACAAGGCGCTGTACGAGGGCTGGGGCGTGACCCTGCCCGGCGGCGTCTGC
CCCGAGGTCGGTGTCGGCGGGCATGTGTCCGGCGGCGGTTACGGGCCTCTGTCCCGGCGC
TACGGACTGTCGGTCGACCATCTGTACGCGGTCGAGGTGGTGGTCGCGGGCAGCGACGGC
AAGGCTCGTACGATCGTCGCGACCAGCCGCCGCAGCGACCCGAACCGCGACCTGTGGTGG
GCGCACACCGGTGGTGGCGGCGGTCAGTTCGGCATCGTGACCAGGTTCTGGTTCCGCACG
CCGGGCGCGTCGGGAGCCCCCACCGCGCTGCTGCCCAAGCCGCCCGCCCGGATGCGCAAG
TCCCTTGTGACCTGGCAGTGGTCGGACATCACCGAGGCCGGCTTCACCCGGCTGCTGCTC
AACCACGGTGCCTGGCACGCGGCCAACAGCGGGCCCGACTCGCCGTACGCCACCATGCAC
AGCTCCTTCCAACTGCGTTCGTCGCTGGTCGGGTCGTTCGTGCTGGAAATCCGGATGGAC
GCGACGCTCGCCGCCACCCCGAAGCTGTTCGACGACTACCTGGCCGCGGTGTCGGCCGGT
GTCGGTGTCACGCCGGCGGTCGACACACGGGAGATGCCCTGGGTGGAGGCGGCCACCGAG
CCGACGCCCCCGTTCGGCACGTACTCCCGGCACAAGTCCATCGGCGGGCATCTGCGCAAG
CCGCTGACGGCAGCTCAGGTCGGCGTCGTGTACCGGTCGTTGACGGATCCCGAGCACTCC
ACCGGCGCCGGTCTCGTGTACCTCGCGGCATACGGCTGCAAGATCAACACGGTGTCGTCG
TCGGCGACGGCCGTGGCCCAGCGGGACTCCATCTTCAAGGTCTGGTACTCGACCAACTGG
TCCGAGCCGTCGGCCGACGCGGCCGAGGTCGAGTGGATCAGAACGCTGCGCAAGGGCGTC
CACAGCGCCACGGGCGGCTTCCCGGTCCCGAACGACCAGCAGGACGGCGGATACGTCAAC
TACCCCGACGTGGACATCAGGGACCCGTACCAGAACTCCTCGGGCGTGCCCTGGTACACG
ATCCTCTACAAGGACAACCACCCCCGGCTGCAGCGGGTCAAGAACACGTACGACCCGCGC
AACGTCTTCAGGCACGGCCTCTCGTTGAACCCGTCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR006094 FAD linked oxidase, N-terminal (Domain)
[70-202] 9e-21 PF01565
PF01565   FAD_binding_4
IPR006311 Twin-arginine translocation pathway, signal sequence (Conserved_site)
[1-30] PS51318
PS51318   TAT
IPR012951 Berberine/berberine-like (Domain)
[478-528] 3.1e-14 PF08031
PF08031   BBE
IPR016166 FAD-binding, type 2 (Domain)
[63-243] PS51387
PS51387   FAD_PCMH
[48-244] 3.59998852907783e-36 SSF56176
SSF56176   FAD-binding_2
IPR016168 FAD-linked oxidase, FAD-binding, subdomain 2 (Domain)
[125-239] 1.1e-07 G3DSA:3.30.465.20
G3DSA:3.30.465.20   FAD-linked_oxidase_FAD-bd_sub2
SignalP
[1-36] 0.983 Signal
Eukaryota   
[1-30] 1 Signal
Bacteria, Gram-negative   
[1-36] 1 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Sch475_00460 Sch475_00460   Sch475_00480 Sch475_00480
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