Sch475_00540 Sch475_00540   last last

Sch475_00550 : CDS information

close this sectionLocation

Organism
StrainSCC-2136
Entry nameSch 47554
Contig
Start / Stop / Direction77,076 / 74,161 / - [in whole cluster]
77,076 / 74,161 / - [in contig]
Locationcomplement(74161..77076) [in whole cluster]
complement(74161..77076) [in contig]
TypeCDS
Length2,916 bp (971 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative beta-galactosidase
Product (GenBank)beta-galactosidae
Gene
Gene (GenBank)schA34
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[17085966] Angucyclines Sch 47554 and Sch 47555 from Streptomyces sp. SCC-2136: cloning, sequencing, and characterization. (Mol Cells. , 2006)
comment
angucyclines Sch 47554 and Sch 47555の生合成に関連したentire gene clusterの
クローニング、シークエンス、配列解析からの特徴づけ。

schA34はtableにも本文にも記載なし。
同定された55ORFsのうち、tableにあるのは50.
Related Reference
ACC
Q56307
PmId
[8088532] ( , )
comment
Blast 32nd, id34%, 1e-141
Thermotoga maritima_lacZ
Beta-galactosidase(Lactase)(EC 3.2.1.23)

Thermotoga maritimaの11-kb fragmentをE. coliのlacZ deletion stainに相補する事により、lacZ遺伝子を同定した文献。
ACC
Q59140
PmId
[7811090] ( , )
comment
Blast 130th, id35%, 1e-128
Arthrobacter sp._lacZ
Beta-galactosidase(Lactase)(EC 3.2.1.23)

E.coliで発現・精製、活性測定
ACC
P00722
PmId
[1350782] ( , )
[11732897] ( , )
comment
Blast 176th, id33%, 1e-126
E.coli_lacZ
Beta-galactosidase(Lactase)(EC 3.2.1.23)

[PMID: 1350782](1992)
active site: Glu-537

[PMID: 11732897](2001)
Glu537 binds to the galactosyl moiety.

close this sectionSequence

selected fasta
>putative beta-galactosidase [beta-galactosidae]
MRRDHLIALRPWEAPEVTSWGRLAMNAVDRRSGALSLDGSWHFQLLPSPMGCSGEWTRTE
VPGTWTTQSGDDLPQYTNAQMPWGEFPPASPAANPTGIYERSVDIPAEWAGRRIVLQVGA
AESVLLVHVNGKPVGISKDSHLAAEFDLSDVVRPGDPATVRFTVVKWSDASHIEDQDQWW
HAGITRSVLLYATDPLYLADVTVRARRDGRLRVDCQVRDATGPLPRGWYVTGALEGQLLA
QDTEFDRFNAEDMRVSDFLGEARLRGTVENVWPWTAETPELYDLTVRLHRADGTVADTSH
HRVGFRDVEIRGRDLLVNGERVYIRGVNRHDFHPLTGRTVTADDMRADLVVMKRFGFNAI
RTAHYPNDPTLYDLADELGFYVVDEANIESHDHAHEIADDPRYLPAFVDRVSRMVLRDKN
HPSVIIWSLGNESDYGANHDAAAGWVRRHDPTRPIQYEGAAKLDWADPTTASDIVCPMYA
PIEDCVAHARSGKQTMPLIQCEYSHAMGNSNGTLADTWAAIESTPGLQGGFIWEFWDHGI
LQRVNDGRPAGRGGAGLYEHGVAAPGHRWAYGGDFGETIHDGAFIADGVVFPDRTPKPVM
YEHREIAAPVRLTYDQGVLLVHNRQHFRDLEWLAAEWCLVLADGTTVTAPAELPDVLPGE
SAAVPLPFPVQEGESWLTLRVVTADDEAWAPLGTEVCLPQVRLGAAVAEATPEPASGPAV
TLDADALLVHPLLTSAPVLSLWRAPTDNDELGGMAARWHDWGLDELVRKVVDVREEGPRV
VVVAEYATGAGPVRHEQVFTPVAGGLRVEESAELPEGLDDVARVGTVFETVAGLDVLDWY
GQGPWESYPDRSTGAPVGHHSLPVDELFTPYLRPQESGGRHGVRRFTLSAPDATGLTVEL
GAPGQVSVNRYRAGDLAAAAHHDELVPRPGCVVHLDAAHRGLGTASCGPDTTAEYLVTPG
THRWAWTLRVL
selected fasta
>putative beta-galactosidase [beta-galactosidae]
ATGCGACGCGATCACCTGATCGCCCTGCGCCCCTGGGAGGCACCGGAGGTGACCTCCTGG
GGGCGGCTGGCCATGAACGCGGTGGACCGGCGCTCCGGTGCGCTGTCCCTGGACGGCTCC
TGGCACTTCCAGCTGCTGCCCTCGCCCATGGGCTGCTCGGGCGAGTGGACCCGGACCGAG
GTGCCGGGCACCTGGACCACCCAGAGCGGCGACGACCTGCCGCAGTACACCAACGCGCAG
ATGCCGTGGGGCGAATTCCCGCCCGCCTCGCCCGCCGCCAACCCGACGGGCATATACGAG
CGTTCCGTCGACATCCCCGCCGAGTGGGCCGGACGCCGGATCGTGCTCCAGGTCGGCGCG
GCCGAGAGCGTGCTCCTGGTCCATGTGAACGGCAAACCGGTCGGCATCTCCAAGGACTCC
CACCTCGCGGCCGAGTTCGACCTCTCCGACGTCGTACGCCCCGGTGACCCGGCGACCGTA
CGGTTCACCGTCGTCAAGTGGTCCGACGCCTCGCACATCGAGGACCAGGACCAGTGGTGG
CACGCCGGTATCACCCGCTCGGTGCTGCTGTACGCGACCGACCCGCTGTATCTCGCGGAC
GTGACCGTGCGGGCGCGGCGGGACGGGCGGCTCCGGGTCGACTGCCAGGTGCGCGACGCG
ACGGGACCGCTGCCGCGGGGGTGGTACGTCACCGGGGCGCTGGAGGGCCAACTCCTAGCC
CAGGACACCGAGTTCGACCGCTTCAACGCCGAGGACATGCGGGTGTCCGACTTCCTCGGT
GAGGCCCGGCTGCGGGGCACCGTCGAGAACGTATGGCCGTGGACCGCCGAGACACCCGAG
CTGTACGACCTGACCGTGCGCCTGCACCGGGCCGACGGTACGGTCGCCGACACCTCCCAC
CACCGGGTCGGGTTCCGGGACGTCGAGATCCGCGGCCGGGACCTGCTGGTCAACGGCGAA
CGCGTCTACATCCGGGGCGTCAACCGGCACGACTTCCACCCGCTGACCGGCCGCACGGTC
ACCGCGGACGACATGCGCGCGGACCTGGTCGTCATGAAGCGCTTCGGCTTCAACGCGATC
CGCACCGCCCACTACCCGAACGACCCGACGCTGTACGACCTCGCCGACGAGCTGGGCTTC
TATGTCGTGGACGAGGCGAACATCGAGTCGCACGACCACGCCCACGAGATCGCCGACGAC
CCCCGCTATCTGCCCGCCTTCGTGGACCGGGTCTCGCGCATGGTGCTGCGCGACAAGAAC
CACCCGTCGGTCATCATCTGGTCGCTCGGCAACGAGTCCGACTACGGAGCCAACCACGAC
GCGGCGGCGGGCTGGGTCCGGCGCCACGACCCGACCCGACCCATCCAGTACGAGGGCGCG
GCCAAGCTCGACTGGGCGGATCCGACGACCGCGTCCGACATCGTCTGCCCGATGTACGCG
CCGATCGAGGACTGTGTCGCGCACGCCCGGTCGGGGAAGCAGACCATGCCGCTCATCCAG
TGCGAGTACTCGCACGCCATGGGCAACAGCAACGGAACGCTCGCCGACACCTGGGCGGCC
ATCGAGTCCACCCCAGGTCTTCAGGGCGGCTTCATCTGGGAGTTCTGGGACCACGGCATT
CTCCAGCGTGTGAACGACGGAAGACCTGCCGGGCGTGGGGGCGCCGGGCTGTACGAGCAC
GGAGTCGCCGCGCCCGGCCATCGCTGGGCCTACGGCGGCGACTTCGGCGAGACCATCCAC
GACGGGGCGTTCATCGCCGACGGCGTGGTGTTCCCGGACCGCACGCCCAAGCCCGTGATG
TACGAGCACCGGGAGATCGCGGCACCGGTCCGCCTCACCTATGACCAGGGCGTGCTCCTG
GTCCACAACCGGCAGCACTTCCGGGACCTGGAATGGCTGGCCGCCGAGTGGTGCCTGGTC
CTCGCGGACGGCACCACGGTGACGGCACCGGCCGAGCTGCCCGACGTACTGCCGGGCGAG
TCCGCGGCGGTGCCACTGCCGTTCCCCGTGCAGGAGGGCGAGTCCTGGCTGACGCTGCGG
GTCGTCACCGCGGACGACGAGGCCTGGGCTCCCCTCGGCACCGAGGTGTGTCTGCCGCAG
GTCCGGCTGGGCGCGGCCGTCGCCGAGGCGACGCCGGAGCCCGCGTCCGGCCCGGCCGTC
ACGCTGGACGCGGACGCACTCCTGGTCCACCCGCTGCTGACCTCCGCGCCCGTCCTCTCG
CTGTGGCGGGCGCCCACCGACAACGACGAACTGGGCGGCATGGCGGCCCGCTGGCACGAC
TGGGGCCTCGACGAGCTCGTACGCAAGGTCGTGGACGTCCGTGAGGAGGGCCCTCGGGTC
GTCGTGGTCGCCGAGTACGCGACCGGGGCCGGGCCCGTCCGCCACGAGCAGGTGTTCACC
CCGGTCGCCGGCGGCCTCCGGGTCGAGGAGTCGGCCGAACTGCCGGAGGGCCTCGACGAC
GTGGCCCGCGTCGGCACGGTCTTCGAGACGGTCGCCGGGCTCGACGTCCTGGACTGGTAC
GGGCAGGGCCCCTGGGAGTCCTACCCGGACCGGAGCACGGGCGCGCCCGTCGGGCACCAC
TCGCTTCCGGTGGACGAGCTGTTCACCCCTTATCTGCGGCCTCAGGAGAGCGGCGGCCGG
CACGGTGTGCGGCGGTTCACGCTGTCGGCGCCGGACGCCACGGGGCTGACCGTGGAGCTC
GGGGCGCCGGGGCAGGTCTCCGTCAACCGGTACCGGGCCGGGGACCTGGCGGCTGCCGCG
CACCACGACGAGCTGGTGCCGCGGCCCGGCTGTGTCGTGCACCTCGACGCCGCGCACCGG
GGGCTCGGCACGGCGTCGTGCGGGCCCGACACCACCGCCGAGTACCTCGTCACGCCGGGC
ACGCACCGCTGGGCGTGGACGCTGCGCGTGCTCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR004199 Beta galactosidase small chain/ domain 5 (Domain)
[732-968] 3.7e-47 PF02929
PF02929   Bgal_small_N
[702-969] 2.2999842048857e-81 SM01038
SM01038   Bgal_small_N
IPR006101 Glycoside hydrolase, family 2 (Family)
[121-136] 8.40001703163685e-28 PR00132 [317-331] 8.40001703163685e-28 PR00132 [348-366] 8.40001703163685e-28 PR00132 [418-433] 8.40001703163685e-28 PR00132 [496-511] 8.40001703163685e-28 PR00132
PR00132   GLHYDRLASE2
IPR006102 Glycoside hydrolase, family 2, immunoglobulin-like beta-sandwich (Domain)
[254-306] 9.50000000000001e-09 PF00703
PF00703   Glyco_hydro_2
[196-307] 1.60000240695997e-17 SSF49303 [604-709] 6.69999687625999e-24 SSF49303
SSF49303   Glyco_hydro_2Ig
IPR006103 Glycoside hydrolase, family 2, TIM barrel (Domain)
[308-610] 3.90000000000001e-88 PF02836
PF02836   Glyco_hydro_2_C
IPR006104 Glycoside hydrolase, family 2, N-terminal (Domain)
[35-194] 2.99999999999998e-37 PF02837
PF02837   Glyco_hydro_2_N
IPR008979 Galactose-binding domain-like (Domain)
[9-195] 9.60004130884281e-48 SSF49785
SSF49785   Gal_bind_like
IPR011013 Glycoside hydrolase-type carbohydrate-binding (Domain)
[714-971] 3.99998544139406e-64 SSF74650
SSF74650   Gal_mut_like
IPR013781 Glycoside hydrolase, catalytic domain (Domain)
[308-606] 5.09999999999992e-91 G3DSA:3.20.20.80
G3DSA:3.20.20.80   Glyco_hydro_cat
IPR013812 Glycoside hydrolase, family 2/20, immunoglobulin-like beta-sandwich domain (Domain)
[184-306] 6.70000000000001e-15 G3DSA:2.60.40.320 [609-706] 2.5e-19 G3DSA:2.60.40.320
G3DSA:2.60.40.320   Glyco_hydro_2/20_Ig-like
IPR014718 Glycoside hydrolase-type carbohydrate-binding, subgroup (Domain)
[731-970] 9.9e-64 G3DSA:2.70.98.10
G3DSA:2.70.98.10   Glyco_hydro_42_D5
IPR017853 Glycoside hydrolase, superfamily (Domain)
[308-606] 6.00001679190159e-86 SSF51445
SSF51445   Glyco_hydro_cat
IPR023230 Glycoside hydrolase, family 2, conserved site (Conserved_site)
[358-383] PS00719
PS00719   GLYCOSYL_HYDROL_F2_1
IPR023232 Glycoside hydrolase, family 2, active site (Active_site)
[418-432] PS00608
PS00608   GLYCOSYL_HYDROL_F2_2
SignalP No significant hit
TMHMM No significant hit
Sch475_00540 Sch475_00540   last last
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