Tauto_00120 : CDS information

Location

Organism	Streptomyces spiroverticillatus
Strain
Entry name	Tautomycin
Contig	EF990140
Start / Stop / Direction	76,705 / 78,075 / + [in whole cluster] 76,705 / 78,075 / + [in contig]
Location	76705..78075 [in whole cluster] 76705..78075 [in contig]
Type	CDS
Length	1,371 bp (456 aa)

Annotation

Category	3.4 other modification
Product	putative esterase
Product (GenBank)	putative type B carboxylesterase
Gene
Gene (GenBank)	ttmK
EC number	3.1.1.-
Keyword	dialkylmaleic anhydride moiety
Note
Note (GenBank)
Reference	ACC C0SMW5 PmId [18708355] Characterization of the tautomycin biosynthetic gene cluster from Streptomyces spiroverticillatus unveiling new insights into dialkylmaleic anhydride and polyketide biosynthesis. (J Biol Chem. , 2008) comment tautomycin (TTM) biosynthetic gene clusterについての文献。 ttmK(456a.a.): esterase ttmKを不活化すると、TTMの生産が見られないことを確認している。機能としては、dialkylmaleic anhydride moietyをpolyketide chainにcouplingするものと予想されている。
Related Reference	ACC Q9L378 PmId [11178722] Cloning and characterization of a bacterial cell-bound type B carboxylesterase from Bacillus sp. BP-7. (Curr Microbiol. , 2001) comment <Type B carboxylesterase, Bacillus sp. BP-7, BLAST 6; id=33%> carboxylesteraseと相同性のあるORFを含んだ領域をクローニングし、短鎖脂肪酸のesters選択的に機能するesteraseであることを確認している。

Sequence

>putative esterase [putative type B carboxylesterase]
MVETSSGSIRGFLDRGVPNWRGVPYGRVGHRFRPPEPVVPVERIDARHWGPVSWQLPIIL
GQTPSTVSPDATESENCLNLNIWSPDPNPDARRPVLMWLHFGSRAYGSGSTSTNDVWHFA
ARHDAVVVTANYRIGAWGWLYLGALDPDFKDSANLSMLDQIQALRWVKENVAAFGGDPDN
VTLFGLSSGSSDVAALLGAPAAKGLFHKAALYSGAQPPMAEERAARFAEDFLESAGPLAA
TPADLAQLPNVGLRTIHRRMLKQATSKSHGPAMIRYGPFVDGNVLPRSPLESLEMGLMAD
VPLLVSNVTEESGAWDAWNAIDTAYLRAFPEHDQSLPHKEKIDALSELIWVGPAKKLLAA
QHKGGAPVWYQQFDYAPSTNWRTVLAYPELANRPVHGADVACLFLDPEGTVGSDEDRAVG
AQDQAALLALARDGRVPWTPWTPESPAPHRIGVPAD

>putative esterase [putative type B carboxylesterase]
GTGGTGGAGACGTCGTCCGGCTCGATTCGGGGCTTCCTGGACCGGGGTGTCCCGAACTGG
CGCGGCGTGCCGTACGGTCGGGTCGGGCACCGGTTCCGGCCACCGGAACCGGTCGTCCCC
GTCGAGCGGATCGACGCACGCCACTGGGGCCCGGTCTCCTGGCAGCTGCCGATCATCCTC
GGCCAGACGCCCTCGACGGTGTCCCCCGACGCGACCGAGAGCGAAAACTGTCTCAACCTC
AACATCTGGTCGCCCGACCCGAACCCGGACGCGCGCCGGCCGGTGCTGATGTGGCTGCAC
TTCGGCAGCCGGGCCTACGGCAGCGGCAGCACGTCGACCAACGACGTCTGGCACTTCGCC
GCCCGGCACGACGCGGTAGTGGTCACCGCCAACTACCGGATCGGGGCCTGGGGCTGGCTC
TATCTGGGCGCGCTGGACCCGGACTTCAAGGACAGCGCCAACCTTTCCATGCTCGACCAG
ATCCAGGCGCTGCGCTGGGTCAAGGAGAACGTGGCCGCCTTCGGCGGCGACCCGGACAAC
GTCACGTTGTTCGGCCTGTCCTCCGGCTCGTCGGACGTGGCGGCTCTGCTGGGTGCTCCG
GCGGCCAAGGGGCTGTTCCACAAGGCGGCGCTCTACAGCGGCGCCCAGCCGCCGATGGCC
GAGGAGCGAGCCGCCCGGTTCGCCGAGGACTTCCTCGAGTCCGCCGGGCCGCTGGCGGCG
ACGCCGGCGGATCTCGCGCAGCTGCCCAACGTGGGGCTGCGCACCATCCATCGGCGGATG
CTGAAGCAGGCCACGTCCAAGTCCCACGGCCCGGCCATGATCCGGTACGGACCGTTCGTC
GACGGCAACGTCCTGCCCCGCAGCCCGCTCGAATCGCTGGAAATGGGGCTCATGGCCGAC
GTTCCGCTGCTCGTCTCGAACGTCACCGAGGAGAGCGGGGCGTGGGACGCCTGGAACGCC
ATCGACACGGCCTACCTGCGCGCGTTCCCCGAGCACGACCAGTCGCTGCCCCACAAGGAG
AAGATCGACGCCCTGTCCGAGCTGATCTGGGTGGGGCCGGCCAAAAAGCTGCTCGCCGCC
CAGCACAAGGGCGGCGCCCCGGTGTGGTACCAACAGTTCGACTACGCGCCCTCCACCAAC
TGGCGGACCGTGCTCGCCTATCCCGAGCTGGCGAACCGGCCGGTCCACGGCGCCGACGTG
GCCTGCCTGTTCCTCGACCCGGAAGGCACCGTCGGCTCCGACGAGGACCGTGCGGTCGGT
GCGCAGGACCAGGCGGCGCTGCTCGCCCTGGCCAGGGACGGGCGGGTGCCGTGGACGCCG
TGGACGCCGGAGTCACCGGCCCCGCACCGGATCGGCGTACCGGCGGACTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR002018 Carboxylesterase, type B (Domain) PF00135 COesterase IPR019826 Carboxylesterase type B, active site (Active_site) PS00122 CARBOXYLESTERASE_B_1
SignalP	No significant hit
TMHMM	No significant hit

Tauto_00110 Tauto_00130