Tauto_00180 Tauto_00180   Tauto_00200 Tauto_00200

Tauto_00190 : CDS information

close this sectionLocation

Organism
Strain
Entry nameTautomycin
Contig
Start / Stop / Direction84,723 / 83,662 / - [in whole cluster]
84,723 / 83,662 / - [in contig]
Locationcomplement(83662..84723) [in whole cluster]
complement(83662..84723) [in contig]
TypeCDS
Length1,062 bp (353 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.1 modification hydroxylation
Producthydroxylase
Product (GenBank)predicted hydroxylase
Gene
Gene (GenBank)ttmM
EC number
Keyword
  • dialkylmaleic anhydride moiety
  • C-3'
Note
Note (GenBank)
Reference
ACC
PmId
[18708355] Characterization of the tautomycin biosynthetic gene cluster from Streptomyces spiroverticillatus unveiling new insights into dialkylmaleic anhydride and polyketide biosynthesis. (J Biol Chem. , 2008)
[19281218] Functional characterization of ttmM unveils new tautomycin analogs and insight into tautomycin biosynthesis and activity. (Org Lett. , 2009)
comment
[PMID: 18708355](2008)
tautomycin (TTM) biosynthetic gene clusterについての文献。

ttmM(353a.a.): alpha-ketoglutarate-dependent hydroxylase

ttmMの機能実験は無いが、Fe(II)-alpha-ketoglutarate oxygenase domainを持つtaurine catabolism dioxygenase TauDとの相同性より、dialkylmaleic anhydride moiety biosynthesisにおけるC-3' hydroxylationに関与していると推測している。

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[PMID: 19281218](2009)
putative C3' hydroxylaseをコードするttmMを不活化した株では、3つの新規3'-deshydroxy TTM analogsを蓄積。よって提唱機能が支持される。

また、TTMの特徴であるprotein phosphatase抑制作用にC3' OH moietyが重要であること、protein phosphatase抑制はTTM誘導細胞死を操縦する独占的機構ではないことが明らかになっている。

close this sectionSequence

selected fasta
>hydroxylase [predicted hydroxylase]
MSILLNVEPGVSRAPDQEPWLHELTVPEAAEIDEAFATLLASGKSDFAAGEADFPLPRVG
PKLRCILRSLEDEPGFALVRGIPVAGKSEHEVRRLYWGLGMYLGVPLIQNNSDSHMVDIR
DEGRAGRLRVHSSNQHIGFHIDSTDIVGLLCRRAAAHGGTSLVVSAEAVRREISWVYPDL
LPALYEPLPFADVASPDEHHPDFFLCPVFGRHEGRTTTRFYLRRILRSQDNSAAPRLTDR
QRRAIEAVEEIAARPDLVTAMEFQPGDLQLINNHLVLHGRTTFDSEEADTGRHLLRMWWS
VPSSRPLPPEFEAAWGTREPGTLRGAARRWQLHGEFGEFQRRQADELGVVIPA
selected fasta
>hydroxylase [predicted hydroxylase]
ATGTCGATTCTGCTGAACGTGGAACCCGGAGTGTCGCGCGCGCCGGACCAGGAGCCGTGG
CTGCACGAGCTGACCGTGCCGGAGGCGGCCGAGATCGACGAAGCGTTCGCCACGCTGCTC
GCGAGCGGCAAGTCCGACTTCGCCGCCGGCGAGGCCGATTTCCCGCTGCCGAGGGTGGGG
CCGAAGCTGCGGTGCATCCTGCGGTCGTTGGAGGACGAGCCGGGTTTCGCGCTGGTCCGC
GGCATCCCGGTCGCAGGGAAGAGCGAGCACGAGGTCCGCCGCCTCTACTGGGGGCTCGGC
ATGTACCTCGGGGTGCCGCTCATCCAGAACAACTCCGACTCCCACATGGTCGACATCCGC
GACGAGGGCCGCGCGGGCAGGCTGCGGGTCCACAGCTCGAACCAGCACATCGGGTTCCAC
ATCGACTCGACGGACATCGTCGGGTTGCTCTGCCGGCGAGCCGCCGCCCACGGCGGCACC
AGCCTCGTCGTGAGTGCCGAAGCGGTGCGGCGGGAGATCTCGTGGGTGTACCCCGACCTG
CTGCCCGCGCTGTACGAACCGCTTCCGTTCGCCGACGTCGCCTCGCCGGACGAGCACCAC
CCCGATTTCTTCCTGTGCCCGGTGTTCGGCCGCCACGAGGGGCGGACCACGACGCGGTTC
TACCTCCGCCGGATCCTGCGCAGCCAGGACAACTCCGCGGCGCCGCGGCTGACCGACCGC
CAGCGGCGCGCCATCGAGGCGGTCGAGGAGATCGCGGCCCGGCCGGACCTGGTCACCGCC
ATGGAGTTCCAGCCGGGTGATCTGCAGCTGATCAACAACCACCTGGTGCTGCACGGCCGG
ACGACGTTCGACAGCGAAGAGGCGGACACCGGACGGCACCTGCTGCGCATGTGGTGGTCG
GTGCCGTCGAGCAGGCCGCTGCCGCCGGAGTTCGAGGCCGCCTGGGGCACCAGGGAACCC
GGCACGCTGCGGGGCGCGGCCCGGCGCTGGCAGCTGCACGGGGAGTTCGGCGAGTTCCAG
CGGAGGCAGGCCGACGAACTCGGGGTCGTCATCCCCGCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR003819 Taurine catabolism dioxygenase TauD/TfdA (Family)
[53-298] 6.60000000000002e-28 PF02668
PF02668   TauD
SignalP
[1-29] 0.03 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Tauto_00180 Tauto_00180   Tauto_00200 Tauto_00200
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