Tauto_00240 : CDS information

Location

Organism	Streptomyces spiroverticillatus
Strain
Entry name	Tautomycin
Contig	EF990140
Start / Stop / Direction	90,853 / 89,450 / - [in whole cluster] 90,853 / 89,450 / - [in contig]
Location	complement(89450..90853) [in whole cluster] complement(89450..90853) [in contig]
Type	CDS
Length	1,404 bp (467 aa)

Annotation

Category	3.4 other modification
Product	putative dehydratase
Product (GenBank)	putative MmgE/PrpD family protein
Gene
Gene (GenBank)	ttmR
EC number
Keyword	dialkylmaleic anhydride moiety
Note
Note (GenBank)
Reference	ACC C0SMX7 PmId [18708355] Characterization of the tautomycin biosynthetic gene cluster from Streptomyces spiroverticillatus unveiling new insights into dialkylmaleic anhydride and polyketide biosynthesis. (J Biol Chem. , 2008) comment tautomycin (TTM) biosynthetic gene clusterについての文献。 ttmR(467a.a.): dehydratase ttmRを不活化すると、TTMの生産が見られないことを確認している。機能としては、MmgE/PrpD familyに属することから、dialkylmaleic anhydride moiety biosynthesisにおけるC-4'/C-5' olefinの導入に関与すると予想されている。
Family/Domain	FD IPR005656 comment [IPR005656] MmgE/PrpD (Family) Function GO:0047547 2-methylcitrate dehydratase activity

Sequence

>putative dehydratase [putative MmgE/PrpD family protein]
MTPAERLGALAELVANDGLPASRRSDVAGRVLDLLGNCLAALSERPAEAVAATVEEWGGS
PQATAIGSATRLPAPSAALLNGVLAHSLDFDDTHLPSVLHPSAAVVPAALAVAEQTDASG
PAFSDAVAVGVEVTCRLGMAGYDEQNANSVFFDRGQHATAICGTVGAAVAAGMLRGLSAA
RLTSAIGIACSMGSGIIEANRTGGTVKRIHCGWAAHAGVVAAALAGNGITGPPTVLEGRF
GLLRAFCGDRFDLEAVTRDLGERWESDRIFVKPYPCNHFTHAGVEAALRIRRRDVDPAII
TELTLGVPEPVLRTIAEPIEDKRRPLSGYHGAFSGPYTVAAALLGGGGLGLSHADFTDTA
VADPARLALAARVTPVADPECTRIFPHQFPAVLTVRLADGTVLEERVRHNLGGPENPLSA
ADLTAKFRMNARRAVDDAVAAELAALVTGLASDTRPAAITCLLRSAT

>putative dehydratase [putative MmgE/PrpD family protein]
GTGACCCCGGCCGAACGGCTGGGCGCGCTGGCCGAACTCGTCGCGAACGACGGACTGCCG
GCCAGCCGGCGGAGCGACGTCGCCGGCCGGGTGCTGGACCTGCTCGGCAACTGCCTCGCC
GCGCTTTCGGAGCGGCCGGCCGAGGCCGTCGCGGCCACGGTGGAGGAGTGGGGCGGTTCG
CCGCAGGCCACGGCGATCGGCTCGGCCACCCGCCTGCCCGCGCCGTCGGCCGCGCTGCTC
AACGGCGTGCTTGCGCACTCACTGGACTTCGACGACACACACTTGCCTTCGGTGCTGCAC
CCGTCAGCCGCGGTCGTGCCGGCGGCACTCGCCGTCGCGGAACAGACCGACGCGTCCGGG
CCCGCTTTCTCCGATGCCGTGGCCGTCGGGGTCGAGGTGACCTGCCGGCTGGGCATGGCC
GGCTACGACGAACAGAACGCGAACTCGGTGTTCTTCGACCGCGGCCAGCACGCCACCGCG
ATCTGCGGCACGGTCGGCGCCGCCGTCGCGGCCGGGATGCTGCGCGGGCTGAGCGCCGCT
CGGCTGACCTCGGCCATCGGCATCGCGTGCAGCATGGGTTCCGGGATCATCGAGGCCAAC
CGGACGGGCGGCACGGTCAAACGGATTCACTGCGGCTGGGCGGCGCACGCCGGGGTGGTG
GCCGCAGCGCTCGCCGGGAACGGGATCACCGGGCCGCCGACCGTGCTGGAAGGCCGGTTC
GGCCTGCTGCGCGCGTTCTGCGGCGACCGGTTCGACCTCGAAGCCGTGACCCGGGACCTC
GGCGAGCGGTGGGAGAGCGACCGGATCTTCGTGAAACCCTATCCCTGCAACCACTTCACC
CACGCCGGGGTGGAGGCCGCGTTGCGGATCCGGCGGCGCGATGTCGATCCCGCCATCATC
ACCGAGCTCACCCTCGGCGTGCCCGAACCGGTGCTGCGCACCATCGCCGAGCCGATCGAG
GACAAACGCCGGCCGCTGTCCGGTTACCACGGCGCGTTCAGCGGGCCGTATACGGTGGCC
GCCGCGTTGCTCGGCGGAGGCGGCCTCGGCCTGTCCCACGCCGACTTCACCGACACCGCG
GTGGCCGATCCGGCCCGCCTCGCGCTGGCCGCGAGGGTGACCCCGGTGGCCGACCCGGAG
TGCACGCGGATTTTCCCGCACCAGTTCCCGGCCGTGCTCACCGTGCGGCTCGCCGACGGC
ACCGTGCTCGAGGAACGGGTCCGGCACAACCTGGGCGGCCCGGAAAATCCGTTGTCCGCC
GCGGACTTGACGGCCAAGTTCCGCATGAACGCGCGGCGCGCGGTGGACGACGCGGTGGCC
GCTGAACTCGCCGCCCTGGTGACCGGGCTGGCTTCGGACACCAGGCCGGCGGCGATCACC
TGCCTGCTGCGTTCGGCGACCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005656 MmgE/PrpD (Family) PF03972 MmgE_PrpD SSF103378 MmgE_PrpD
SignalP	No significant hit
TMHMM	No significant hit

Tauto_00230 Tauto_00250