Tetrn_00270 : CDS information

Location

Organism	Streptomyces sp.
Strain	NRRL 11266
Entry name	Tetronomycin
Contig	AB193609
Start / Stop / Direction	103,414 / 105,168 / + [in whole cluster] 103,414 / 105,168 / + [in contig]
Location	103414..105168 [in whole cluster] 103414..105168 [in contig]
Type	CDS
Length	1,755 bp (584 aa)

Annotation

Category	5.2 function unknown
Product	AMP-binding family protein
Product (GenBank)	putative acyltransferase family protein
Gene
Gene (GenBank)	tmn20
EC number
Keyword
Note	It is supposed that this ORF is not involved in tetronomycin biosynthesis.
Note (GenBank)
Reference	ACC Q1MX62 PmId [18404760] Analysis of the tetronomycin gene cluster: insights into the biosynthesis of a polyether tetronate antibiotic. (Chembiochem. , 2008) comment Streptomyces sp. NRRL 11266におけるtetronomycin (TMN) gene clusterに関する文献。 tmn20(584 aa): acyltransferase-family protein tmn20についてはTable 1.以外の文献中で特に触れられていないが、TMN産生に関与するTMN gene clusterの外側に位置しているtmnDI 破壊株でTMN産生に影響が見られないことより、tmnDI のさらに外側に位置している本ORFはTMN産生に関与しないと考えられている。
Family/Domain	FD IPR000873 comment [IPR000873] AMP-dependent synthetase/ligase (Domain) Pfam> Family: AMP-binding (PF00501)

Sequence

>AMP-binding family protein [putative acyltransferase family protein]
MTIAVSTEEIHGDRAGFQPETDPAPVAMTPAATIGAALLDLADSDPDRPLAYVEGSGDPV
RLTAAELAERAGAAATTLAEHGVRRGDRVGVCMDTSADAYATLHGCFLLGAIPFVSEPPL
ATMRRQRWADRLRLLISRAEPRAVVVAPEFLDAVTEPCKEAGVAIVEPPFEGGLDLATAQ
AKPDDIALLQFTSGTTGDSRGVTLTHESIFANATAIGERAYRAGDLIVSWLPMHHDMGLI
GLNLAPLLHGLPVASMPPLSFAVRPERWLWAMHRYRGTFTSAPNFAYRMCASNIPDAKLA
GLDLSAWRVAGNGAEVVQANTLRLFAERMAAYGYRERSMMPCYGMAEITLAATMCEPGEP
LTTLTVSRDRLAADGEVVEAEAGAADAQELVSSGTALTGMDLRVVDDEGADLPDRRQGTI
LLRTDSMMSGYYRQPEETAKVLADDGWLNTGDQGFTDAGQTYVTGRIKDVVIIAGSNYHP
YAFESAAASVEGLRANSIAAVGCPDEERSTEALVLVVESKSHADADAVAQIREGVIQAVS
AETGLRPDRVEVVPRGTLPKTPSGKLQRRHVVDALVDGTLPTSH

>AMP-binding family protein [putative acyltransferase family protein]
ATGACAATTGCCGTTTCGACCGAGGAAATCCACGGCGACCGCGCGGGATTTCAGCCCGAG
ACGGACCCGGCACCGGTCGCGATGACGCCGGCCGCCACGATAGGCGCCGCACTGCTCGAC
CTGGCGGACAGCGACCCCGACCGCCCGCTCGCGTACGTCGAGGGCTCCGGCGACCCCGTG
CGGCTGACGGCGGCGGAGCTGGCGGAGCGCGCGGGCGCCGCCGCGACGACCCTCGCGGAG
CACGGCGTACGGCGCGGAGACCGCGTCGGCGTCTGCATGGACACCAGCGCCGACGCGTAC
GCCACGCTGCACGGCTGCTTCCTGCTGGGCGCCATCCCGTTCGTCAGCGAGCCGCCGCTG
GCGACGATGCGCCGGCAGCGCTGGGCCGACCGGCTGCGGCTGCTGATCAGCCGCGCGGAG
CCGCGCGCCGTGGTCGTCGCGCCGGAGTTCCTCGACGCGGTGACGGAGCCGTGCAAGGAG
GCGGGCGTCGCGATCGTCGAGCCGCCGTTCGAGGGCGGCCTCGACCTCGCCACCGCGCAG
GCCAAGCCCGACGACATCGCACTGCTGCAGTTCACCTCGGGCACCACGGGCGACAGCCGG
GGCGTGACCCTGACGCACGAGTCGATCTTCGCGAACGCCACGGCCATCGGCGAGCGCGCG
TACCGCGCCGGCGACCTGATCGTGAGCTGGCTGCCGATGCACCACGACATGGGCCTGATC
GGGCTGAACCTCGCGCCGCTGCTGCACGGACTGCCGGTGGCCAGCATGCCCCCGCTGTCC
TTCGCGGTACGGCCCGAGCGGTGGCTGTGGGCGATGCACCGCTACCGCGGGACGTTCACC
TCGGCGCCCAACTTCGCGTACCGCATGTGCGCCTCGAACATCCCCGACGCCAAGCTGGCC
GGCCTCGACCTGAGCGCCTGGCGGGTCGCCGGCAACGGCGCGGAGGTCGTGCAGGCGAAC
ACCCTGCGCCTGTTCGCCGAGCGCATGGCCGCGTACGGCTACCGCGAGCGTTCGATGATG
CCCTGCTACGGCATGGCGGAGATCACCCTGGCCGCGACGATGTGCGAGCCCGGCGAGCCG
CTGACCACGCTCACCGTCAGCCGGGACCGGCTGGCCGCGGACGGCGAGGTCGTGGAGGCC
GAGGCCGGCGCCGCCGACGCGCAGGAACTGGTGTCCTCCGGCACCGCGCTGACGGGCATG
GATCTGCGCGTCGTGGACGACGAGGGCGCCGACCTGCCGGACCGCAGGCAGGGCACCATC
CTGCTGCGCACCGACTCGATGATGTCCGGCTACTACCGCCAGCCCGAGGAGACCGCGAAG
GTCCTGGCCGACGACGGCTGGCTGAACACCGGCGACCAGGGCTTCACCGACGCCGGGCAG
ACCTACGTCACCGGCCGCATCAAGGACGTCGTCATCATCGCCGGGTCCAACTACCACCCG
TACGCCTTCGAGTCGGCCGCCGCCTCGGTGGAGGGCCTGCGGGCGAACTCCATCGCCGCG
GTCGGCTGCCCCGACGAGGAGCGCAGCACGGAGGCGCTGGTGCTGGTGGTCGAGTCGAAG
AGCCACGCGGACGCCGACGCCGTCGCCCAGATCCGCGAGGGCGTCATCCAGGCCGTCTCC
GCCGAGACCGGGCTGCGTCCCGACCGGGTGGAGGTGGTGCCGCGGGGCACGCTGCCGAAG
ACCCCGAGCGGCAAGCTCCAGCGGCGCCACGTGGTGGACGCGCTCGTCGACGGCACGCTG
CCGACGAGCCACTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000873 AMP-dependent synthetase/ligase (Domain) PF00501 AMP-binding IPR020845 AMP-binding, conserved site (Conserved_site) PS00455 AMP_BINDING IPR025110 Domain of unknown function DUF4009 (Domain) PF13193 DUF4009
SignalP	No significant hit
TMHMM	No significant hit

Tetrn_00260 Tetrn_00280