Virg_00180 Virg_00180   Virg_00200 Virg_00200

Virg_00190 : CDS information

close this sectionLocation

Organism
StrainMAFF 10-06014
Entry nameVirginiamycin
Contig
Start / Stop / Direction58,367 / 54,744 / - [in whole cluster]
29,991 / 26,368 / - [in contig]
Locationcomplement(54744..58367) [in whole cluster]
complement(26368..29991) [in contig]
TypeCDS
Length3,624 bp (1,207 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)hybrid non ribosomal peptide synthetase-polyketide synthase
Gene
Gene (GenBank)virG
EC number
Keyword
  • virginiamycin M
Note
Note (GenBank)
Reference
ACC
PmId
[17350183] Characterization of biosynthetic gene cluster for the production of virginiamycin M, a streptogramin type A antibiotic, in Streptomyces virginiae. (Gene. , 2007)
comment
virginiamycin M生合成gene clusterの報告。

virG: Hybrid PKS-NRPS

module6_PKS (KR, ACP)
module7_PKS (KS, ACP)
module8_NRPS (Cy, Cy-)


module6_PKSは、2つのORFに別れている。
VirF(Virg_00200): KS-unk-
VirG(Virg_00190): -KR-ACP

VirFのC末端でKSドメインのすぐ下流に存在するunknown領域は、完全長のmoduleになるために、VirFとVirG間のprotein-protein interactionをしているのではないかと考察している。

module8_NRPS について、論文中では2つのcyclization(Cy) domainがこのORF上に存在すると記述されているが、InterProの結果からこのドメインに対応すると考えられるCondensation domainは隣のORF(Virg_00180_VirH)に存在していた。

close this sectionPKS/NRPS Module

M6
7
KR56..240
ACP342..410
KS477..842
ACP1131..1200

close this sectionSequence

selected fasta
>polyketide synthase [hybrid non ribosomal peptide synthetase-polyketide synthase]
MLTADGTPDARRLLAEIAAPAAEHGHLADLRSARRLRRVLVPVPLPGTRPFVRQDGVYLV
SGGAGGIGLALARHLTDRPGTRVVLCGRTAWDDLAEATRSAVSGSDRLRYARADVTDPEA
AAALVAEVVRTEGALHGVFHAAGVVRDGYLVRKDPRDVADVLAPKILGARALDEATAALP
LDAFVLFSSVAAVTGNLGQSDYAFANGFLDGFATRRAGQVARGERHGRTLSVQWPLWDVP
GMSIPEPVLEVVAQHTGMAPLPAAVGLAALERLLAADGPEVVSLFHGDAATWRAHLAALH
LERPAAAGQVTAAAPAAPAPSDTPVVASADPAAADDGRRAELADRVSRTVADTIGRPAGS
IGGHTSLESMGLDSVMIRALASRLSAEVAPVGPEMLFGLRDLDELVDHLVAARPVPVAEP
ATPAVPPAPAATAATVFAPVAPADPAVVPAAPAALPVPAAAPASASRTLPAPSADDRFAI
IGISGRYPQAPDLDAFWQNLLNGKDTASDLPTDRWPDAAGVNARGHFLEGVDAFDPTFFG
LSAHDGTLLDPQERLFLEVAWEALEDAGYTGSRLHDLVAPDGERRSVGVFAGITSSDYKL
LGAERWAAGHREMPSGHYWSLPNRLSYLLDLRGPSQPVDTACSSSLVALHLALDALRRGE
CAAALVGGVNLYVHPSRFRMLRRSGFLAEDGLCRSFGAGGAGFGPGEGGGAVVVKPLAVA
LADGDTVHAVVRGSAVAHGGRTNGFTAPSPWAQARVLRAALRNSGTDPETVNVIEAHGTG
TELGDPVELAGLQDAYGSGRVPCSLGSVKSAVGHGESAAGIAALTKVVLQLRHGELVPTL
HADPVNPGLRLEDTRFVLQHTPGRWERLTGADGSPLPRRAGISSFGAGGVNAHVIVEEYL
PEPHGRAAAAEPGRPELVLLSAPTREHLAATADRLARRLEGPEAPADLRAVAYSLRTGRS
AMDCRLAVVATDTAGLAAALGAFARSADAGEGESAVRYADLRDGRRAHRDLDTVPETTAF
LADLWRNRRLHQLGELWLSGLDIERAAPREPGTRLVPLPTSAFLRRRLWITDPVTADTTP
TPDPLPTPDPLPVPLSAPAPAPAPAPQPAPTTTPAPAAAPAAAPARSGEAVTEQLSRLVA
GFVPDAAGSVDPDRTLLEHGIDSINLMNLRFEITERFGRTLPLQLLSESTVPVLAAHLSA
DRAHDRA
selected fasta
>polyketide synthase [hybrid non ribosomal peptide synthetase-polyketide synthase]
GTGCTGACCGCGGACGGCACGCCGGACGCGAGGCGGTTGCTCGCCGAGATCGCCGCGCCG
GCCGCCGAGCACGGGCACCTGGCCGACCTCCGCTCCGCCCGGCGGCTGCGCCGGGTGCTG
GTACCGGTTCCGCTGCCCGGCACCCGCCCCTTCGTGCGCCAGGACGGCGTGTACCTGGTC
AGCGGCGGTGCGGGCGGCATCGGCCTGGCACTGGCCCGGCACCTGACGGACCGTCCGGGC
ACCCGCGTGGTGCTCTGCGGACGGACCGCGTGGGACGACCTCGCCGAAGCGACCCGCAGC
GCCGTCTCCGGCAGCGACCGCCTGCGCTACGCCAGGGCCGACGTCACCGACCCCGAGGCC
GCCGCCGCGCTCGTGGCCGAGGTCGTCCGGACCGAGGGCGCCCTGCACGGCGTCTTCCAC
GCCGCCGGCGTGGTGCGCGACGGCTACCTGGTGCGCAAGGACCCCCGGGACGTGGCCGAC
GTGCTGGCGCCCAAGATCCTCGGTGCGCGGGCGCTGGACGAGGCGACCGCGGCCCTCCCG
CTGGACGCGTTCGTGCTGTTCTCCTCGGTGGCCGCGGTGACCGGCAACCTCGGCCAGAGC
GACTACGCGTTCGCCAACGGCTTCCTCGACGGCTTCGCCACCCGCCGTGCCGGGCAGGTG
GCGCGCGGTGAGCGGCACGGCAGGACGCTGTCCGTGCAGTGGCCGCTCTGGGACGTCCCC
GGCATGAGCATTCCCGAGCCGGTCCTGGAAGTGGTGGCCCAGCACACCGGCATGGCGCCG
CTGCCCGCAGCCGTCGGGCTCGCGGCTCTGGAACGCCTGCTGGCCGCCGACGGCCCCGAG
GTCGTCTCGCTCTTTCACGGCGACGCGGCCACGTGGCGGGCCCACCTGGCCGCCCTCCAC
CTGGAGCGGCCGGCCGCGGCCGGCCAGGTGACGGCCGCCGCTCCCGCCGCTCCCGCCCCT
TCCGACACCCCCGTCGTCGCGTCGGCCGATCCTGCTGCGGCGGATGACGGCCGGCGCGCC
GAACTGGCCGACCGGGTCAGCCGGACGGTGGCCGACACCATCGGCCGCCCCGCGGGCAGC
ATCGGCGGTCACACCTCGCTCGAGTCGATGGGGCTGGACTCCGTCATGATCCGCGCCCTC
GCCTCCCGGCTCAGCGCCGAAGTGGCACCGGTCGGGCCGGAGATGCTCTTCGGCCTGCGC
GACCTGGACGAGCTGGTCGACCACCTGGTCGCCGCCCGGCCGGTCCCCGTAGCGGAACCG
GCCACACCGGCGGTCCCGCCGGCACCCGCCGCCACGGCGGCCACGGTCTTCGCACCGGTG
GCTCCGGCGGACCCGGCCGTTGTCCCGGCCGCCCCGGCCGCCCTGCCCGTCCCCGCCGCC
GCGCCGGCATCCGCCTCCCGTACGCTGCCGGCGCCGTCCGCCGATGACCGCTTCGCGATC
ATCGGCATCAGCGGCCGCTACCCGCAGGCGCCCGACCTGGACGCCTTCTGGCAGAACCTG
CTGAACGGCAAGGACACCGCCTCCGACCTGCCCACCGACCGCTGGCCGGACGCCGCGGGC
GTCAACGCCCGGGGCCACTTCCTCGAAGGCGTGGACGCGTTCGACCCGACGTTCTTCGGA
CTGTCCGCCCATGACGGGACCCTGCTCGACCCGCAGGAACGGCTGTTCCTGGAGGTCGCC
TGGGAGGCGCTGGAGGATGCCGGATACACCGGCTCGCGTCTGCACGACCTCGTCGCGCCG
GACGGCGAGCGCCGCAGCGTCGGCGTCTTCGCCGGGATCACCTCCAGCGACTACAAGCTG
CTGGGCGCCGAGCGCTGGGCAGCCGGACACCGGGAGATGCCCTCCGGGCACTACTGGAGC
CTGCCCAACCGGCTGTCCTACCTGCTGGACCTGCGCGGTCCGAGCCAGCCGGTCGACACC
GCCTGCTCGTCCTCCCTGGTCGCGCTGCACCTCGCGCTCGACGCGCTCCGCCGCGGCGAG
TGCGCGGCCGCTCTGGTCGGCGGGGTCAACCTGTACGTCCACCCCTCCCGGTTCCGGATG
CTGCGCCGGTCCGGCTTCCTGGCCGAGGACGGCCTGTGCCGCAGCTTCGGCGCCGGCGGG
GCCGGCTTCGGCCCGGGCGAGGGCGGCGGTGCCGTCGTGGTGAAACCGCTCGCCGTCGCC
CTGGCCGACGGTGACACCGTGCACGCGGTGGTCCGCGGCAGCGCGGTCGCACACGGCGGG
CGGACCAACGGCTTCACCGCGCCGAGCCCGTGGGCGCAGGCACGGGTGCTCCGTGCCGCG
CTGCGGAACTCCGGCACCGACCCGGAGACCGTCAACGTGATCGAGGCGCACGGCACCGGG
ACGGAACTCGGCGACCCGGTCGAGCTGGCCGGCCTCCAGGACGCGTACGGCTCCGGCCGC
GTGCCCTGCTCGCTGGGGTCGGTCAAGTCCGCGGTGGGGCACGGCGAGTCGGCGGCCGGC
ATCGCCGCGCTGACCAAGGTGGTCCTCCAACTGCGCCACGGCGAGCTGGTCCCCACCCTG
CACGCCGACCCGGTCAACCCGGGCCTGCGCCTGGAGGACACCCGCTTCGTCCTCCAGCAC
ACCCCGGGCCGCTGGGAGCGACTGACCGGCGCGGACGGCAGCCCGCTGCCGCGACGGGCG
GGCATCAGCTCCTTCGGCGCCGGCGGAGTCAACGCCCACGTCATCGTCGAGGAGTACCTG
CCCGAGCCGCACGGCCGCGCGGCCGCCGCCGAGCCGGGCCGCCCGGAACTGGTCCTGCTC
TCCGCCCCGACGCGCGAGCACCTGGCGGCCACCGCCGACCGCCTCGCGCGCCGGCTGGAG
GGCCCGGAGGCGCCCGCCGACCTGCGGGCGGTGGCGTACAGCCTGCGCACCGGCCGGTCC
GCCATGGACTGTCGCCTCGCCGTGGTGGCGACCGACACCGCCGGCCTGGCGGCCGCGCTC
GGCGCCTTCGCCCGCTCGGCCGACGCCGGCGAGGGGGAGTCCGCCGTGCGGTACGCCGAC
CTGCGGGACGGCCGCCGTGCCCATCGGGACCTGGACACGGTGCCCGAGACGACCGCGTTC
CTCGCCGACCTGTGGCGCAACCGGCGCCTGCACCAACTCGGCGAGCTCTGGCTGTCGGGC
CTCGACATCGAGCGTGCCGCGCCCCGTGAGCCGGGCACCCGGCTGGTGCCGCTCCCCACG
TCGGCGTTCCTGCGGCGCCGGCTCTGGATCACCGACCCGGTCACGGCGGACACCACGCCC
ACACCGGACCCTCTGCCCACACCGGACCCCCTGCCCGTACCGCTGTCCGCTCCGGCACCG
GCACCGGCCCCCGCCCCCCAGCCCGCGCCGACCACCACCCCGGCCCCGGCAGCCGCGCCC
GCCGCAGCGCCTGCCAGATCCGGGGAAGCGGTGACCGAGCAGCTGTCGCGCCTCGTCGCG
GGCTTCGTACCCGACGCGGCGGGGTCGGTCGACCCCGACCGCACCCTGCTCGAACACGGC
ATCGACTCGATCAACCTGATGAACCTGCGCTTCGAGATCACCGAGCGGTTCGGGCGGACC
CTGCCGCTGCAGTTGCTCAGCGAGTCGACCGTCCCCGTGCTCGCCGCCCACCTGTCCGCC
GACCGCGCCCACGACCGGGCCTGA
[6] KR56..240
[6] ACP342..410
[7] KS477..842
[7] ACP1131..1200
[6] KR166..720
[6] ACP1024..1230
[7] KS1429..2526
[7] ACP3391..3600

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR009081 Acyl carrier protein-like (Domain)
[340-411] 8.30000000000004e-20 G3DSA:1.10.1200.10 [1130-1201] 8.30000000000004e-20 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[345-409] 7.3e-07 PF00550 [1134-1197] 1.5e-06 PF00550
PF00550   PP-binding
[342-410] PS50075 [1131-1200] PS50075
PS50075   ACP_DOMAIN
[335-492] 1e-13 SSF47336 [1127-1201] 5.79999653854781e-10 SSF47336
SSF47336   ACP_like
IPR013968 Polyketide synthase, KR (Domain)
[56-238] 1.9e-52 PF08659
PF08659   KR
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[477-718] 1.9e-67 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[728-842] 8.30000000000004e-40 PF02801
PF02801   Ketoacyl-synt_C
IPR016038 Thiolase-like, subgroup (Domain)
[476-731] 6.99999999999989e-77 G3DSA:3.40.47.10 [732-900] 8.89999999999994e-54 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[468-898] 4.30000170645869e-78 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
[55-274] 3.2e-43 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[633-649] PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain)
[56-240] 7.2000271977897e-48 SM00822
SM00822   PKS_KR
SignalP
[1-51] 0.088 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Virg_00180 Virg_00180   Virg_00200 Virg_00200
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