Virg_00210 Virg_00210   Virg_00230 Virg_00230

Virg_00220 : CDS information

close this sectionLocation

Organism
StrainMAFF 10-06014
Entry nameVirginiamycin
Contig
Start / Stop / Direction62,518 / 61,778 / - [in whole cluster]
34,142 / 33,402 / - [in contig]
Locationcomplement(61778..62518) [in whole cluster]
complement(33402..34142) [in contig]
TypeCDS
Length741 bp (246 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.4 other modification
Productputative enoyl-CoA hydratase
Product (GenBank)enoyl-CoA hydratase
Gene
Gene (GenBank)virD
EC number4.2.1.-
Keyword
  • virginiamycin M
  • C-12 methylation
Note
Note (GenBank)
Reference
ACC
PmId
[17350183] Characterization of biosynthetic gene cluster for the production of virginiamycin M, a streptogramin type A antibiotic, in Streptomyces virginiae. (Gene. , 2007)
comment
virginiamycin M生合成gene clusterの報告。

本ORFは、VirB, VirC, VirEと共にC12の位置でのメチル基の取り込みに関与すると予測されている。
VirD and/or E(putative enoyl-CoA hydratases)により生じる二重結合の異性化はC11 alpha,beta-unsaturated thioesterに属すると予測された。

本ORFの機能解析はしていない。
Related Reference
ACC
P40805
PmId
[16757561] Convergence of isoprene and polyketide biosynthetic machinery: isoprenyl-S-carrier proteins in the pksX pathway of Bacillus subtilis. (Proc Natl Acad Sci U S A. , 2006)
comment
PKSH_BACSU(P40805)
Bacillus subtilis_Putative polyketide biosynthesis enoyl-CoA hydratase pksH(pksH)

HMG formationに続くステップは、isopentenyl derivative形成のためのdehydration and decarboxylationであるとの推測に基づいてPksH and PksI の活性を調べた。PksHがisopentenyl productのdehydrationを起こす。

close this sectionSequence

selected fasta
>putative enoyl-CoA hydratase [enoyl-CoA hydratase]
MRVVRGRGLLRAVLDRPERRNPIDAGLLTSLARALDQAESDQDCRVFVLSSTGEDFCAGT
DLSGGDPAPEPLPDGAELPYWTLLERLTRSPLATVAVVDGRATAGGVGLAAACDLVLAGE
RARFRLTEVLAGLVPAMALPFVARRTGEQRAFAATLRAEEFDAGAAHRVGLADLAGPRAE
DLLPPVLAGLGRTDRSTTAALKEYRARLFPRDARLGHDASRLLIERFAAPGTGQLLARLR
EAGAAA
selected fasta
>putative enoyl-CoA hydratase [enoyl-CoA hydratase]
GTGCGCGTCGTCCGCGGCCGCGGGCTGCTGCGGGCCGTCCTGGACCGCCCCGAGCGGCGC
AACCCGATCGACGCCGGACTGCTCACCTCGCTCGCCCGGGCCCTCGACCAGGCCGAGAGC
GACCAGGACTGCCGGGTGTTCGTCCTGTCGTCGACGGGCGAAGACTTCTGCGCGGGCACC
GACCTGTCCGGCGGCGACCCCGCTCCCGAGCCGCTGCCGGACGGCGCCGAACTGCCCTAC
TGGACGCTGCTGGAGCGGCTGACCCGCAGCCCGCTCGCCACCGTCGCCGTCGTCGACGGC
CGGGCCACCGCGGGCGGGGTCGGCCTGGCCGCCGCCTGCGACCTCGTCCTGGCGGGGGAG
CGGGCCCGCTTCCGGCTCACCGAGGTGCTGGCCGGGCTGGTCCCGGCGATGGCCCTGCCG
TTCGTCGCCCGCCGGACCGGCGAGCAGCGGGCGTTCGCCGCGACCCTGCGCGCCGAGGAG
TTCGACGCCGGCGCCGCCCACCGGGTCGGCCTCGCCGACCTGGCCGGCCCGCGCGCCGAG
GACCTGCTGCCCCCGGTGCTGGCCGGACTCGGCAGGACCGACCGGAGCACCACCGCGGCG
CTCAAGGAGTACCGCGCCCGGCTGTTCCCCCGGGACGCCCGCCTGGGCCACGACGCCTCG
CGGCTGCTGATCGAGCGGTTCGCCGCACCGGGAACCGGGCAGCTGCTGGCGCGGCTGCGC
GAGGCCGGAGCGGCGGCATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001753 Crotonase, core (Domain)
[9-180] 4.80000000000001e-32 PF00378
PF00378   ECH
SignalP
[1-34] 0.312 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Virg_00210 Virg_00210   Virg_00230 Virg_00230
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