Actino_00010 : CDS information

Location

Organism	Streptomyces coelicolor
Strain	A3(2) (=NBRC 15146)
Entry name	Actinorhodin
Contig	X62373
Start / Stop / Direction	1,213 / 29 / - [in whole cluster] 1,213 / 29 / - [in contig]
Location	complement(29..1213) [in whole cluster] complement(29..1213) [in contig]
Type	CDS
Length	1,185 bp (394 aa)

Annotation

Category	5.4 hypothetical protein
Product	hypothetical protein
Product (GenBank)	hydroxylacyl-CoA dehydrogenase
Gene	actVI-ORFB
Gene (GenBank)	ORFB
EC number
Keyword
Note	It is supposed that this ORF is not involved in actinorhodin biosynthesis.
Note (GenBank)
Reference	ACC Q53924 PmId [7929165] DNA sequence and functions of the actVI region of the actinorhodin biosynthetic gene cluster of Streptomyces coelicolor A3(2). (J Biol Chem. , 1994) [10805574] Chemical characterisation of disruptants of the Streptomyces coelicolor A3(2) actVI genes involved in actinorhodin biosynthesis. (J Antibiot (Tokyo). , 2000) comment [PMID: 7929165](1994) actinorhodin biosynthetic gene clusterのactVI regionに関する報告。 ORFB: Unknown ORFB破壊はactinorhodin産生に影響しない。 DB検索では有意なAA類似性を示すタンパクなし。 -- [PMID: 10805574](2000) ORFB破壊株ではwildに匹敵するactinorhodin生産力あり。よって明らかにactinorhodin生合成に直接関与しない。たぶんact clusterに含まれない。

Sequence

>hypothetical protein [hydroxylacyl-CoA dehydrogenase]
MLSVGHGPRHVLVVARPHPDEPVGGATALALAELVAGGDRRSPATDPEAVTWDIVLCLDP
DGAALSEGIETATAGPAPSLASYYRAAFRPVAAEQPEWAPIVGRPLPESRALFDVIDDLR
PVLQCSLHNVDAGGTWAQLTRDLPGLAAPFAASAAALGVPVQHGTYDALYWENPAPGIHV
LPPPGSTARPAAGSEDIGLSTWCAPQRYGGATAIVEVPLWATAPADDPTPHADPARALRA
LSGLVREGGRQVTAVFERARGLLPAPEDSPPVRVLEWMAEDLYDLVADSWGPLVRQAEAG
PRTDGSPALTTAGIGALEVVARRQPLRAAGLLLRLLHAADAPAAAGLHHELDALFTAWCT
DFETSLRLRWVPVHAQVELQARTVVAAAECALTP

>hypothetical protein [hydroxylacyl-CoA dehydrogenase]
ATGCTGTCGGTGGGCCACGGACCCCGGCACGTCCTGGTGGTGGCCCGGCCGCACCCGGAC
GAGCCGGTGGGCGGCGCGACGGCACTGGCGCTGGCGGAGCTGGTCGCAGGCGGCGACCGG
CGCTCCCCGGCGACCGACCCCGAGGCCGTCACCTGGGACATCGTCCTGTGCCTCGACCCG
GACGGCGCCGCGCTGAGCGAGGGCATCGAGACCGCGACGGCCGGCCCCGCCCCGTCCCTG
GCGTCGTACTACCGTGCCGCCTTCCGCCCGGTCGCCGCGGAGCAGCCGGAGTGGGCCCCG
ATCGTCGGCCGGCCACTCCCGGAGAGCCGTGCCCTGTTCGACGTGATCGACGACCTGCGC
CCCGTCCTCCAGTGCTCCCTGCACAACGTCGACGCGGGCGGCACCTGGGCCCAGCTGACC
CGCGACCTCCCCGGACTCGCCGCCCCCTTCGCGGCGTCCGCCGCCGCCCTGGGGGTCCCG
GTCCAGCACGGCACGTACGACGCCCTGTACTGGGAGAATCCGGCCCCCGGCATCCATGTC
CTGCCGCCGCCGGGCAGCACCGCGCGGCCGGCGGCCGGGTCCGAGGACATCGGCCTGTCC
ACCTGGTGCGCGCCCCAGCGGTACGGCGGTGCCACGGCGATCGTCGAGGTACCGCTGTGG
GCCACCGCCCCGGCGGACGACCCGACCCCGCACGCGGACCCCGCCCGGGCCCTGCGCGCG
CTGTCCGGGCTGGTGCGGGAGGGCGGCCGGCAGGTGACCGCCGTGTTCGAGAGGGCCCGC
GGCCTCCTGCCGGCACCCGAGGACAGCCCACCCGTACGCGTACTGGAGTGGATGGCCGAG
GACCTGTACGACCTGGTGGCCGACTCCTGGGGGCCCCTGGTCCGGCAGGCGGAGGCGGGA
CCGCGCACCGACGGCTCACCCGCGTTGACGACGGCGGGGATCGGCGCCCTGGAGGTCGTC
GCGCGCCGTCAGCCGCTACGCGCGGCGGGACTCCTGCTGCGGCTGCTGCACGCGGCCGAC
GCCCCGGCCGCCGCCGGGCTCCACCACGAGCTGGACGCCCTCTTCACCGCCTGGTGCACG
GACTTCGAGACGTCCCTGCGGCTGCGCTGGGTCCCCGTGCACGCCCAGGTGGAACTCCAG
GCCCGCACGGTCGTGGCGGCGGCGGAGTGCGCGCTGACACCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	No significant hit
SignalP	No significant hit
TMHMM	No significant hit

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