Actino_00160 Actino_00160   Actino_00180 Actino_00180

Actino_00170 : CDS information

close this sectionLocation

Organism
StrainA3(2) (=NBRC 15146)
Entry nameActinorhodin
Contig
Start / Stop / Direction17,080 / 17,865 / + [in whole cluster]
110 / 895 / + [in contig]
Location17080..17865 [in whole cluster]
110..895 [in contig]
TypeCDS
Length786 bp (261 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productpolyketide C-9 ketoreductase
Product (GenBank)
GeneactIII
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • actIII protein
Reference
ACC
PmId
[2469622] Nucleotide sequence, transcription and deduced function of a gene involved in polyketide antibiotic synthesis in Streptomyces coelicolor. (Gene. , 1988)
[2394677] Biosynthesis of anthraquinones by interspecies cloning of actinorhodin biosynthesis genes in streptomycetes: clarification of actinorhodin gene functions. (J Bacteriol. , 1990)
[15458634] The crystal structure of the actIII actinorhodin polyketide reductase: proposed mechanism for ACP and polyketide binding. (Structure. , 2004)
[18205400] Inhibition kinetics and emodin cocrystal structure of a type II polyketide ketoreductase. (Biochemistry. , 2008)
[18691223] Localization of the ActIII actinorhodin polyketide ketoreductase to the cell wall. (FEMS Microbiol Lett. , 2008)
[21506596] Structural and biochemical analyses of regio- and stereospecificities observed in a type II polyketide ketoreductase. (Biochemistry. , 2011)
[24035284] The determinants of activity and specificity in actinorhodin type II polyketide ketoreductase. (Chem Biol. , 2013)
comment
[PMID: 2469622](1988) abstract
actIII を含んだfragmentのシークエンス、配列解析。

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[PMID: 2394677](1990)
anthracyclines 2-hydroxyaklavinoneを産生するStreptomyces galilaeus ATCC 31671にactIII を導入すると、aklavinoneが独占的に形成される。よってActIII がC-9でのketo基の還元することが示される。

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[PMID:15458634](2004)
activeな4量体のS.coelicolor type II polyketide KR (actIII )とその結合cofactor NADP+との結晶構造を測定。観察結果に基づき、ACP and polyketide bindingのモデルを構築。

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[PMID:18205400](2008)
ActKRの基質が直線polyketideなのか、環化されたpolyketideなのかを、不安定な自然基質に代わり、安定な基質候補でスクリーニング。
linear or monocyclic ketones, acetoacetyl-CoA or acetoacetyl-ACPで活性見られず。
bicyclic ketoneのtrans-1-decalone, 2-decalone, and alpha-tetraloneに酵素活性あり。

その他の実験結果も総合し、C9位置特異性は、active siteでのthree-point dockingと、基質のC7-C12環形状による二重の制約の結果である。

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[PMID:18691223](2008)
PKS-KS subunitは細胞膜、ketoreductase_ActIII は細胞壁にある。

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[PMID:21506596](2011)
ActKRの立体特異性について調査し、94-XGG-96 motifが重要であることを確認している。

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[PMID:24035284](2013) abstract
ActKRの位置特異性について調査している。

close this sectionSequence

selected fasta
>polyketide C-9 ketoreductase
MATQDSEVALVTGATSGIGLEIARRLGKEGLRVFVCARGEEGLRTTLKELREAGVEADGR
TCDVRSVPEIEALVAAVVERYGPVDVLVNNAGRPGGGATAELADELWLDVVETNLTGVFR
VTKQVLKAGGMLERGTGRIVNIASTGGKQGVVHAAPYSASKHGVVGFTKALGLELARTGI
TVNAVCPGFVETPMAASVREHYSDIWEVSTEEAFDRITARVPIGRYVQPSEVAEMVAYLI
GPGAAAVTAQALNVCGGLGNY
selected fasta
>polyketide C-9 ketoreductase
ATGGCCACGCAGGACTCCGAAGTCGCACTGGTGACGGGTGCGACCAGCGGAATCGGGCTG
GAGATCGCCCGCAGGCTCGGTAAGGAGGGGTTGCGCGTGTTCGTCTGCGCGCGCGGCGAA
GAAGGACTGCGGACGACGCTGAAGGAGTTGCGGGAGGCGGGCGTGGAGGCGGACGGGCGG
ACCTGCGACGTCCGCTCGGTCCCGGAGATCGAGGCTCTCGTGGCGGCGGTGGTCGAGCGT
TACGGTCCGGTCGACGTGCTGGTGAACAACGCGGGCCGGCCCGGCGGCGGCGCCACGGCC
GAACTCGCCGACGAACTCTGGCTCGATGTCGTGGAGACCAACCTCACCGGCGTGTTCCGG
GTGACCAAACAGGTCCTCAAGGCGGGCGGCATGCTCGAACGGGGCACGGGCCGAATCGTC
AACATCGCCTCGACCGGCGGAAAGCAGGGTGTGGTGCACGCCGCGCCCTACTCCGCCTCG
AAGCACGGCGTGGTCGGCTTCACCAAGGCGCTCGGTCTCGAACTGGCGAGGACCGGCATC
ACGGTGAACGCCGTCTGCCCCGGATTCGTCGAGACGCCGATGGCCGCGTCCGTGCGCGAG
CACTACTCGGACATCTGGGAGGTGTCGACCGAGGAGGCCTTCGACCGGATCACCGCGCGC
GTGCCGATCGGCCGGTACGTGCAGCCGTCCGAGGTGGCGGAGATGGTGGCGTACCTGATC
GGGCCCGGTGCGGCCGCGGTCACCGCGCAGGCGCTGAACGTCTGCGGCGGGCTGGGGAAC
TACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
[82-93] 1.1e-14 PR00080 [137-145] 1.1e-14 PR00080 [157-176] 1.1e-14 PR00080
PR00080   SDRFAMILY
[8-175] 5.6e-38 PF00106
PF00106   adh_short
IPR002347 Glucose/ribitol dehydrogenase (Family)
[8-25] 1e-46 PR00081 [82-93] 1e-46 PR00081 [131-147] 1e-46 PR00081 [157-176] 1e-46 PR00081 [178-195] 1e-46 PR00081 [222-242] 1e-46 PR00081
PR00081   GDHRDH
IPR016040 NAD(P)-binding domain (Domain)
[5-258] 9e-79 G3DSA:3.40.50.720
G3DSA:3.40.50.720   no description
IPR020904 Short-chain dehydrogenase/reductase, conserved site (Conserved_site)
[144-172] PS00061
PS00061   ADH_SHORT
SignalP No significant hit
TMHMM No significant hit
Actino_00160 Actino_00160   Actino_00180 Actino_00180
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