Actino_00190 Actino_00190   Actino_00210 Actino_00210

Actino_00200 : CDS information

close this sectionLocation

Organism
StrainA3(2) (=NBRC 15146)
Entry nameActinorhodin
Contig
Start / Stop / Direction20,631 / 20,891 / + [in whole cluster]
2,626 / 2,886 / + [in contig]
Location20631..20891 [in whole cluster]
2626..2886 [in contig]
TypeCDS
Length261 bp (86 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase acyl carrier protein subunit
Product (GenBank)Acyl carrier protein
GeneactI-ORF3
Gene (GenBank)actIORF3
EC number
Keyword
  • type II PKS
Note
Note (GenBank)
Reference
ACC
PmId
[1527048] Nucleotide sequence and deduced functions of a set of cotranscribed genes of Streptomyces coelicolor A3(2) including the polyketide synthase for the antibiotic actinorhodin. (J Biol Chem. , 1992)
[16285746] Self-malonylation is an intrinsic property of a chemically synthesized type II polyketide synthase acyl carrier protein. (Biochemistry. , 2005)
[19361520] Probing the Interactions of early polyketide intermediates with the Actinorhodin ACP from S. coelicolor A3(2). (J Mol Biol. , 2009)
[19381365] Preliminary kinetic analysis of acyl carrier protein-ketoacylsynthase interactions in the actinorhodin minimal polyketide synthase. (Mol Biosyst. , 2009)
[25406716] Modeling linear and cyclic PKS intermediates through atom replacement. (J Am Chem Soc. , 2014)
comment
[PMID: 1527048](1992)
actinorhodin biosynthetic gene clusterのactI領域の配列解析。

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[PMID: 19361520](2009) abstract
ACP-中間体の相互作用を見てる。

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[PMID:19381365](2009) abstract
actinorhodin PKSのACPとKSの間の相互作用をkinetic assaysを使用して調査。

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他にも実験論文多数あり。

Simpson TJ.らはact ACPがself-acylation(malonyl-ACP形成)すると言っている[PMID:9479478](1998), [PMID:9862793](1998), [PMID:16285746](2005)。

これと異なり、Khosla C.らはmalonyl-CoA:ACP transacylase(MCAT)が必要で、一次代謝のFA合成geneを動員すると言っている[PMID:9518007](1998), [PMID:10455191](1999)。

close this sectionPKS/NRPS Module

ACP6..79

close this sectionSequence

selected fasta
>polyketide synthase acyl carrier protein subunit [Acyl carrier protein]
MATLLTTDDLRRALVECAGETDGTDLSGDFLDLRFEDIGYDSLALMETAARLESRYGVSI
PDDVAGRVDTPRELLDLINGALAEAA
selected fasta
>polyketide synthase acyl carrier protein subunit [Acyl carrier protein]
ATGGCAACCCTGCTGACCACCGACGATCTGCGCCGCGCCCTCGTGGAGTGCGCCGGTGAG
ACGGACGGGACGGACCTGTCCGGCGACTTCCTCGACCTCCGCTTCGAGGACATCGGGTAC
GACTCGCTCGCCCTGATGGAGACCGCGGCGCGACTCGAGAGCCGGTACGGCGTGTCCATA
CCCGACGACGTCGCCGGCCGCGTCGACACGCCGCGAGAGCTGCTCGACCTGATCAACGGC
GCACTGGCCGAGGCGGCATGA
ACP6..79
ACP16..237

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR006162 Phosphopantetheine attachment site (PTM)
[37-52] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[6-79] 16.283 PS50075
PS50075   ACP_DOMAIN
[24-78] 2.3e-08 PF00550
PF00550   PP-binding
[1-86] 1.1e-15 SSF47336
SSF47336   ACP-like
[6-84] 1.6e-15 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   no description
SignalP No significant hit
TMHMM No significant hit
Actino_00190 Actino_00190   Actino_00210 Actino_00210
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