Salino_00840 : CDS information

Location

Organism	Streptomyces albus subsp. albus
Strain	DSM 41398
Entry name	Salinomycin
Contig	HE586118
Start / Stop / Direction	94,603 / 93,554 / - [in whole cluster] 94,603 / 93,554 / - [in contig]
Location	complement(93554..94603) [in whole cluster] complement(93554..94603) [in contig]
Type	CDS
Length	1,050 bp (349 aa)

Annotation

Category	5.1 general function
Product	putative oxidoreductase
Product (GenBank)	putative Zn-dependent dehydrogenase
Gene
Gene (GenBank)
EC number
Keyword
Note	It is supposed that this ORF is not involved in salinomycin biosynthesis.
Note (GenBank)	orf1
Reference	ACC H1ZZS6 PmId [22076845] A late-stage intermediate in salinomycin biosynthesis is revealed by specific mutation in the biosynthetic gene cluster. (Chembiochem. , 2012) comment monC gene-based hybridisation probeを使って、Streptomyces albus DSM 41398からsalinomycin(sal)生合成gene clusterを同定。 orf1 (349aa) : unknown orf1-3はsal clusterに属さないように見える。
Family/Domain	FD IPR013154 comment [IPR013154] Alcohol dehydrogenase GroES-like (Domain)

Sequence

>putative oxidoreductase [putative Zn-dependent dehydrogenase]
MKAITVVPGDPGQVRVEEVQDAKPAQGELLVEGLLLGLCGTDADIVEEGYGWVPPGQQRL
LLGHESLGSVRDAPPGSGFAPGDLVAGIVRRPDPVPCEPCAHGSWDFCSNGRYTERGIKE
LDGYGSQLWRIEPEYAIKLDPRLGDCGVLLEPASVLAKAWEQIDRFVTRAPLRPRTVLVT
GAGPIGLLAALMGAQRALEVHVLDVNGDEPKAQLVKDLGATFHVGSLDDAKVTPELIVDT
TGHGPLVFDLTGKVAPSGVICLTGIASDSRRTPVPLDESNKEMVLENTVMFGSVNANRRN
FEQAAEALADADLRWLRRLITRKVPLSGFTPEFLHKRPEDVKVVVDLQA

>putative oxidoreductase [putative Zn-dependent dehydrogenase]
ATGAAGGCGATCACGGTCGTCCCCGGAGATCCCGGCCAGGTGCGGGTCGAGGAGGTCCAG
GACGCGAAGCCGGCCCAGGGCGAACTGCTCGTCGAGGGGCTGCTGCTCGGCCTCTGCGGC
ACCGACGCCGACATCGTCGAGGAGGGCTACGGCTGGGTGCCGCCCGGTCAGCAGCGCCTG
CTCCTCGGCCACGAGTCGCTGGGCTCGGTGCGGGACGCCCCGCCGGGCAGCGGCTTCGCC
CCGGGCGACCTAGTGGCGGGCATCGTACGGCGGCCCGACCCGGTGCCCTGCGAGCCCTGC
GCGCACGGCTCCTGGGACTTCTGCAGCAACGGCCGGTACACCGAGCGCGGCATCAAGGAG
CTGGACGGCTACGGCTCCCAGCTGTGGCGCATCGAGCCGGAGTACGCCATCAAGCTCGAC
CCCCGGCTCGGCGACTGCGGGGTCCTCCTGGAACCGGCCTCGGTGCTCGCCAAGGCCTGG
GAACAGATCGACCGCTTCGTCACCCGGGCCCCGCTGCGCCCGAGGACGGTCCTGGTCACC
GGCGCCGGGCCGATCGGCCTGCTGGCGGCGCTGATGGGCGCCCAGCGCGCCCTGGAGGTG
CACGTCCTGGACGTCAACGGGGACGAGCCCAAGGCGCAGCTGGTGAAGGACCTCGGCGCC
ACCTTCCACGTGGGCTCCCTGGACGACGCCAAGGTGACCCCGGAGCTGATCGTCGACACC
ACCGGGCACGGACCGCTGGTCTTCGACCTCACCGGCAAGGTGGCCCCCTCGGGGGTGATC
TGCCTGACCGGCATCGCCTCGGACTCCCGGCGCACCCCCGTGCCGCTGGACGAGAGCAAC
AAGGAGATGGTCCTCGAGAACACGGTGATGTTCGGCTCGGTCAACGCCAACCGGCGCAAC
TTCGAGCAGGCCGCCGAGGCGCTCGCGGACGCGGATCTGCGCTGGCTGCGGCGGCTGATC
ACCCGCAAGGTCCCGCTGAGCGGCTTCACCCCGGAGTTCCTGCACAAGCGGCCCGAGGAC
GTGAAGGTCGTCGTCGATCTGCAGGCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR011032 GroES-like (Domain) SSF50129 GroES-like IPR013154 Alcohol dehydrogenase GroES-like (Domain) PF08240 ADH_N IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 no description
SignalP	No significant hit
TMHMM	No significant hit

Salino_00830 Salino_00850