Salino_00870 : CDS information

Location

Organism	Streptomyces albus subsp. albus
Strain	DSM 41398
Entry name	Salinomycin
Contig	HE586118
Start / Stop / Direction	98,796 / 97,813 / - [in whole cluster] 98,796 / 97,813 / - [in contig]
Location	complement(97813..98796) [in whole cluster] complement(97813..98796) [in contig]
Type	CDS
Length	984 bp (327 aa)

Annotation

Category	5.4 hypothetical protein
Product	hypothetical protein
Product (GenBank)	putative transcriptional regulator
Gene
Gene (GenBank)	salN
EC number
Keyword
Note	It is supposed that this ORF is not involved in salinomycin biosynthesis.
Note (GenBank)
Reference	ACC H1ZZS9 PmId [22076845] A late-stage intermediate in salinomycin biosynthesis is revealed by specific mutation in the biosynthetic gene cluster. (Chembiochem. , 2012) comment monC gene-based hybridisation probeを使って、Streptomyces albus DSM 41398からsalinomycin(sal)生合成gene clusterを同定。 salN (327aa) : putative regulator 詳細記載なし。 PKSの上流はsalNまでがsal clusterに属するとされている。参考)[PMID: 26459865] における、S. albus DSM41398由来salinomycin clusterの異種性発現にsalNは使用されていない。
Related Reference	ACC H6D565 NITE Salino3_00090 PmId [22156425] Cloning and characterization of the polyether salinomycin biosynthesis gene cluster of Streptomyces albus XM211. (Appl Environ Microbiol. , 2012) comment 配列は[H1ZZS9]と全く同じ。株違い。 polyetherに特異的なepoxidaseの配列に基づいた縮重プライマーを用いて、Streptomyces albus XM211からsalinomycin(sln)生合成gene clusterを同定。 Orf9 (327aa) : Regulatory protein 配列解析のみ。 salinomycin生合成におそらく関連しないORFとして挙げられている(Table S3)。

Sequence

>hypothetical protein [putative transcriptional regulator]
MHGYTHLRLGPLDQLRVSVTAHQGATLLSLLADALGGRSQGVPERWRALVRQAAPEGSGA
VLAPLFAPAYSVIPDSITPTACMPDGDVASQCALLADLSPDTLLTELETEFPEFVPPQWQ
PVVERPQEWLRAYARLLGSVWDQFRPVWRGAGALLHRETERVGTAAVSGNLDVLLGSVSS
RFRYGEGSLRLPDQQAAEFSLDGRRLVLVPIVSGSGASMFALDRPDLVWIGYPMPGVGRL
WENCTEPHGGTDALALLLGPRRAALLRACEEPLTMGEIAVRIGCSPATLTYHCAQLEQAG
LLQRERRGQQVRLWRTERGHSVLDLLS

>hypothetical protein [putative transcriptional regulator]
ATGCACGGCTATACGCACTTGCGTCTCGGACCGCTGGACCAGCTGCGCGTCTCGGTGACC
GCACACCAGGGCGCCACCCTGCTCTCACTGCTCGCGGACGCCCTCGGCGGCAGGTCCCAG
GGGGTGCCCGAGCGGTGGCGGGCCCTGGTCAGGCAGGCGGCGCCGGAGGGCTCCGGGGCC
GTGCTCGCCCCGCTCTTCGCCCCCGCGTACTCGGTGATCCCGGACAGCATCACCCCCACC
GCCTGCATGCCCGACGGCGATGTGGCCAGCCAGTGCGCCCTGCTCGCCGACCTCTCGCCG
GACACCCTGCTGACCGAACTGGAGACGGAGTTCCCGGAGTTCGTCCCGCCCCAGTGGCAG
CCGGTGGTGGAGCGCCCCCAGGAGTGGCTGCGGGCCTACGCGCGGCTGCTCGGCTCGGTC
TGGGACCAGTTCCGGCCGGTGTGGCGGGGTGCCGGCGCCCTGCTGCACCGGGAGACCGAG
CGGGTGGGCACCGCCGCGGTGAGCGGGAACCTGGACGTGCTCCTCGGCTCGGTCAGCAGC
CGCTTCCGCTACGGGGAGGGCAGCCTGCGCCTGCCGGACCAGCAGGCGGCCGAGTTCAGC
CTCGACGGCAGGCGTCTCGTGCTGGTGCCGATCGTCTCGGGCAGCGGCGCCTCGATGTTC
GCCCTGGACCGGCCCGACCTGGTGTGGATCGGCTACCCGATGCCGGGAGTCGGGCGGCTC
TGGGAGAACTGCACCGAACCGCACGGCGGCACCGACGCGCTCGCGCTCCTGCTCGGCCCC
CGCCGCGCGGCCCTGCTGCGCGCCTGCGAGGAGCCGCTGACCATGGGCGAGATCGCCGTC
CGGATCGGCTGCAGCCCCGCCACCCTCACCTACCACTGCGCCCAGCTGGAGCAGGCGGGC
CTGCTCCAGCGCGAGCGCCGCGGCCAGCAGGTGCGGCTGTGGCGCACCGAACGCGGCCAC
TCCGTACTCGACCTGCTCTCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain) G3DSA:1.10.10.10 no description
SignalP	No significant hit
TMHMM	No significant hit

Salino_00860 Salino_00880