Salino_00940 : CDS information

Location

Organism	Streptomyces albus subsp. albus
Strain	DSM 41398
Entry name	Salinomycin
Contig	HE586118
Start / Stop / Direction	137,786 / 142,714 / + [in whole cluster] 137,786 / 142,714 / + [in contig]
Location	137786..142714 [in whole cluster] 137786..142714 [in contig]
Type	CDS
Length	4,929 bp (1,642 aa)

Annotation

Category	1.1 PKS
Product	polyketide synthase
Product (GenBank)	type I modular polyketide synthase
Gene
Gene (GenBank)	salAIV
EC number
Keyword
Note
Note (GenBank)
Reference	ACC H1ZZT6 PmId [22076845] A late-stage intermediate in salinomycin biosynthesis is revealed by specific mutation in the biosynthetic gene cluster. (Chembiochem. , 2012) comment monC gene-based hybridisation probeを使って、Streptomyces albus DSM 41398からsalinomycin(sal)生合成gene clusterを同定。 salAIV (1642aa): polyketide synthase KS, AT, KR, ACP module 7のKR domainはactive-site residues Tyr150 and Asn154がそれぞれPhe and Serによって置換されているので実際にはinactiveであるようだ。
Related Reference	ACC H6D572 NITE Salino3_00160 PmId [22156425] Cloning and characterization of the polyether salinomycin biosynthesis gene cluster of Streptomyces albus XM211. (Appl Environ Microbiol. , 2012) comment 配列は[H1ZZT6]と全く同じ。株違い。 polyetherに特異的なepoxidaseの配列に基づいた縮重プライマーを用いてStreptomyces albus XM211からsalinomycin(sln)生合成gene clusterを同定。 SlnA4 (1,642aa) : PKS Module 7 : KS-ATp-KRa-ACP KRa = inactive domain

PKS/NRPS Module

methylmalonyl-CoA

KS	34..410

AT	575..893

kr	1196..1373

ACP	1485..1555

Sequence

>polyketide synthase [type I modular polyketide synthase]
MDNEKKLLDHLKWVTAELRQARQKLREAEADEPEPIAIVSMACRYPGGVRSPEDLWQLVR
EGRDAITGFPTGRGWDLASLYDADPDRLGTSYVREGGFVHDAGDFDAEFFGISPREALAM
DPQQRLLLETSWEAFERAGVDPAAVAGSRTGVFVGTTYTGYGSDREGAEENVEGHLMTGI
ATAVASGRLSYTYGFEGPAVSLDTMCSSSLVALHLAVQALRQKECTLALAGGSQIMSTPD
VYVEFSRQRGLSPDGRCKPFAAAADGTGWSEGVGVLLLERLSDARRNGHRVLAVVRGSAI
NQDGASNGLTAPNGPSQQRVIEQALANARLSPHQVDLIEAHGTGTTLGDPIEAQALLATY
GGSRPEGRPLWLGSVKSNIGHAAAAAGVAGVIKAVMAVREGVLPKSLHIDAPTPEVDWSS
GAVALLTEEREWSVPGEEPRTAAISSFGASGTNAHVLVQYAPEPDPEPAAEAPAAVFTGA
ALPWLLSGRTAEGLRGQARSLHTYASTTATALPGAALGLATTRAALERRGAVLTSGTPGT
PDKDALLTGLDALAEGTPAAGILEGTTVSGADRPVFVFPGQGSQWAGMAVELLDSSSVFA
ARLGECAEALDPFVDWSLVDVLRQTEGAPGFDRVDVVQPALWAVMVSLAEVWRAAGVAPA
AVIGHSQGEIAAAAVSGALSLSDAAKVSALRARALLALAGKGGMVSVADAADSVRERISA
WGERLALASVNGPQSTVVSGDPEALDELMAGCEAEGVRARRINVDYASHGPQVEKIRTEV
LGALSGIEPRTAEVPFLSTVTGEFVTGTELDAEYWYRNLRNTVRFEDAVRQLLDRGHGAF
VEASAHPVLTVGVQETIDAAGAPAFVQGTLRREDGDAARFLASLAEAWTRGVPVNWAIVT
DSSAPPAEDLPTYAFQRRTYWLHGARTGSVQAPVDAVEAEFWDSVENGDIDSLSGSLALE
DSAPLAELLPALSSWRRRRRERGEVDSWRYRIDWQPLAESAPPALEGTWLLVTGDGVEAE
ILKTGESALSAHGATVHPLTLTGEGEREALVRQLLGAEVEHGPFAGVLSLLATAEPERGP
ATTLALVQALADAECEAPLWVATRGAVGTGPEEAPAHPAQAGAWGLGLVAALERPGGWGG
LVDLPAEPDENLAGRLAAALAGQEDQLALRATGTYVRRLARAPLPGSEVRPWEPADTVLV
TGATAPVGARTALLLAASGAKRLLLTVPGGEEQPAALVGELEEAGVQVTLAEWDGRDVTA
LRSLAEAAAADGAPVRGVFHAATRADLAPLDETTAADLAAATAAKTVPARALDEAFGEEV
EAFVLFSSVTSYWGGGEHAAFAAASAELDALAARRRSRGLAATSVAWGVWDLFDAEQNPA
EAAELQARSADRGLPLLDPETAWQALRLSLGRQETAIAVADVDWERFWPLFTSARPAPLL
SDLPEVRGLGRGLAEDTGTGADPGAAEALRSKLAGLSPAEQDRALTDLVCAHAAAVLGHS
SAGAVDAERAFKDLGFDSLTAVGLRNGLGAATGLSLPATLVFDYPTPAAMAGYVRDHLLA
GARQEATAAGVQSGLDQLEADLLSVALDKDERKNLTRRLEGLLSRFKDAQAAADEESVSG
KLDSASDEEIFAFIREEFGRPE

>polyketide synthase [type I modular polyketide synthase]
ATGGACAACGAAAAGAAGCTTCTCGATCACCTCAAGTGGGTGACGGCCGAACTCCGCCAG
GCCCGGCAGAAGCTGCGCGAGGCCGAGGCGGACGAGCCCGAGCCGATCGCCATCGTCTCC
ATGGCCTGCCGCTACCCCGGTGGCGTGCGCTCGCCCGAGGACCTGTGGCAGCTGGTCCGC
GAGGGCCGGGACGCCATCACCGGCTTCCCCACCGGCCGCGGCTGGGACCTGGCCTCGCTC
TACGACGCCGACCCGGACCGGCTCGGCACCAGCTACGTACGCGAGGGCGGCTTCGTCCAC
GACGCGGGCGACTTCGACGCCGAGTTCTTCGGCATCTCCCCGCGTGAGGCGCTTGCCATG
GACCCGCAGCAGCGGCTCCTCCTGGAAACCTCCTGGGAGGCCTTCGAGCGCGCGGGCGTC
GACCCGGCCGCCGTCGCCGGCAGCCGCACCGGCGTCTTCGTCGGCACCACCTACACCGGC
TACGGCAGCGACCGGGAGGGCGCCGAGGAGAACGTCGAGGGCCACCTCATGACCGGCATC
GCGACCGCCGTCGCCTCCGGCCGGCTCTCCTACACCTACGGATTCGAGGGCCCCGCCGTC
TCCCTCGACACCATGTGCTCCTCCTCCCTGGTCGCCCTGCACCTGGCCGTCCAGGCGCTG
CGGCAGAAGGAGTGCACCCTCGCGCTCGCGGGCGGCTCGCAGATCATGTCCACGCCGGAC
GTGTACGTGGAGTTCAGCCGCCAGCGCGGCCTCTCCCCGGACGGCCGCTGCAAGCCCTTC
GCCGCCGCCGCCGACGGCACCGGCTGGTCCGAGGGGGTCGGCGTGCTGCTCCTGGAGCGG
CTCTCCGACGCCCGGCGCAACGGCCACCGGGTGCTCGCCGTGGTCCGCGGCTCCGCGATC
AACCAGGACGGCGCCTCCAACGGGCTCACCGCCCCCAACGGGCCCTCCCAGCAGCGCGTC
ATCGAGCAGGCCCTCGCCAACGCCCGGCTCTCCCCGCACCAGGTCGACCTGATCGAGGCG
CACGGCACCGGCACCACCCTCGGCGACCCCATCGAGGCGCAGGCCCTGCTCGCCACCTAC
GGCGGCAGCCGCCCGGAGGGGCGCCCGCTCTGGCTCGGCTCGGTGAAGTCGAACATCGGC
CACGCGGCCGCCGCGGCGGGTGTCGCGGGTGTCATCAAGGCGGTCATGGCGGTACGCGAG
GGCGTGCTCCCCAAGTCGCTGCACATCGACGCCCCCACCCCGGAGGTCGACTGGTCCTCC
GGCGCCGTCGCCCTGCTCACCGAGGAGCGCGAGTGGTCCGTCCCCGGTGAGGAGCCGCGC
ACCGCCGCGATCTCCTCCTTCGGCGCGAGCGGCACCAACGCCCATGTCCTCGTCCAGTAC
GCCCCCGAGCCCGACCCGGAGCCGGCGGCCGAGGCACCCGCCGCGGTCTTCACCGGCGCC
GCGCTGCCCTGGCTCCTCTCCGGCCGTACCGCCGAGGGACTGCGCGGCCAGGCCCGCTCC
CTGCACACCTACGCCTCCACCACCGCCACCGCACTCCCGGGCGCGGCCCTCGGCCTCGCC
ACCACCCGCGCCGCCCTGGAACGCCGCGGCGCGGTCCTCACCTCCGGCACCCCCGGTACC
CCCGACAAGGACGCCCTGCTCACCGGCCTGGACGCCCTCGCCGAGGGCACCCCGGCGGCC
GGGATCCTCGAAGGCACCACGGTCAGCGGCGCCGACCGCCCCGTCTTCGTCTTCCCGGGT
CAGGGTTCGCAGTGGGCGGGGATGGCGGTCGAACTCCTGGATTCCTCCTCGGTGTTCGCG
GCTCGTCTGGGGGAGTGCGCGGAGGCGCTGGACCCCTTCGTGGACTGGTCCTTGGTGGAT
GTCCTGCGGCAGACCGAGGGTGCTCCCGGTTTCGATCGCGTCGATGTGGTGCAGCCCGCG
CTCTGGGCCGTGATGGTGTCCCTCGCGGAGGTGTGGCGTGCGGCGGGTGTCGCCCCGGCC
GCGGTGATCGGTCACTCGCAGGGTGAGATCGCGGCTGCGGCGGTGTCCGGTGCGCTGTCC
CTGTCGGATGCGGCGAAGGTTTCGGCGTTGCGGGCGCGGGCGTTGCTGGCGCTGGCGGGC
AAGGGCGGGATGGTCTCGGTCGCGGATGCGGCCGATTCCGTACGGGAGCGGATCTCCGCT
TGGGGTGAGCGGCTTGCGCTCGCTTCGGTGAACGGTCCGCAGTCGACGGTGGTTTCGGGT
GATCCCGAGGCGCTGGACGAGTTGATGGCGGGCTGTGAGGCGGAGGGGGTGCGGGCCCGG
CGGATCAATGTGGACTACGCCTCGCACGGCCCCCAGGTGGAGAAGATCCGGACCGAGGTC
CTGGGCGCGCTCTCCGGTATCGAGCCGCGCACCGCCGAGGTGCCCTTCCTGTCCACGGTG
ACCGGCGAGTTTGTGACCGGCACCGAGCTGGACGCCGAGTACTGGTACCGCAACCTGCGC
AACACGGTCCGATTCGAGGACGCCGTACGGCAGTTGCTGGACCGGGGCCACGGGGCCTTC
GTGGAGGCGAGTGCCCATCCGGTGCTGACCGTCGGCGTGCAGGAGACCATCGACGCGGCG
GGCGCGCCCGCCTTCGTCCAGGGCACCCTGCGCCGCGAGGACGGCGACGCGGCCCGCTTC
CTCGCCTCCCTCGCCGAGGCCTGGACCCGTGGTGTGCCCGTCAACTGGGCCATCGTCACG
GACAGTTCGGCGCCCCCGGCCGAGGATCTGCCGACCTACGCCTTCCAGCGCCGCACCTAC
TGGCTGCACGGCGCCCGGACCGGCTCGGTCCAGGCCCCGGTGGACGCCGTGGAGGCCGAG
TTCTGGGACTCCGTGGAGAACGGGGACATCGACTCGCTCTCCGGTTCCCTCGCCCTGGAG
GACAGCGCGCCCCTGGCGGAGCTGCTCCCGGCACTCTCCTCCTGGCGGCGCCGCAGGCGG
GAGCGCGGCGAGGTCGACAGCTGGCGCTACCGGATCGACTGGCAGCCGCTGGCCGAGAGC
GCACCGCCCGCCCTGGAGGGGACCTGGCTGCTCGTCACCGGGGACGGGGTCGAGGCCGAG
ATCCTGAAGACGGGGGAGTCGGCGCTCAGCGCGCACGGCGCCACCGTGCACCCGCTGACC
CTCACGGGGGAGGGCGAACGCGAAGCCCTCGTACGGCAGTTGCTGGGCGCCGAGGTCGAG
CACGGCCCCTTCGCCGGGGTGCTCTCGCTGCTCGCCACCGCGGAGCCGGAGCGGGGGCCC
GCCACCACCCTGGCGCTCGTCCAGGCCCTGGCGGACGCGGAGTGCGAGGCGCCGCTGTGG
GTCGCCACCCGCGGCGCCGTCGGCACCGGACCCGAGGAAGCACCCGCACACCCCGCGCAG
GCGGGGGCCTGGGGCCTCGGCCTGGTCGCCGCCCTGGAGCGGCCCGGCGGCTGGGGCGGC
CTCGTCGACCTGCCCGCCGAGCCGGACGAGAACCTGGCCGGGCGGCTCGCCGCCGCGCTC
GCGGGGCAGGAGGACCAGCTCGCGCTGCGGGCCACCGGCACCTATGTGCGCCGACTGGCG
CGCGCCCCGCTGCCCGGCTCCGAGGTGCGGCCCTGGGAACCCGCCGACACCGTGCTCGTC
ACCGGCGCGACCGCGCCCGTCGGCGCCCGTACCGCCCTGCTGCTCGCGGCCTCCGGCGCC
AAGCGCCTGCTGCTCACCGTGCCCGGCGGGGAGGAGCAACCTGCCGCGCTGGTGGGGGAG
TTGGAGGAAGCCGGGGTCCAGGTCACGCTCGCGGAGTGGGACGGCCGGGACGTCACCGCG
CTGCGGTCCCTCGCGGAGGCGGCCGCCGCGGACGGCGCCCCCGTCCGCGGGGTCTTCCAC
GCCGCCACCCGGGCCGACCTCGCCCCGCTCGACGAGACCACCGCCGCCGACCTCGCCGCC
GCCACCGCGGCGAAGACCGTCCCGGCACGCGCCCTGGACGAGGCCTTCGGCGAGGAGGTC
GAGGCCTTCGTGCTGTTCTCCTCGGTCACCTCCTACTGGGGCGGCGGGGAGCACGCCGCC
TTCGCCGCCGCGAGCGCCGAGCTGGACGCGCTCGCCGCCCGCCGCCGGTCCCGCGGACTC
GCCGCCACCTCGGTGGCCTGGGGCGTCTGGGACCTCTTCGACGCCGAGCAGAACCCGGCG
GAGGCCGCCGAACTCCAGGCCCGCTCCGCGGACCGCGGACTGCCGCTGCTCGACCCCGAG
ACCGCCTGGCAGGCGCTGCGGCTCAGCCTCGGCAGGCAGGAGACCGCGATCGCCGTCGCG
GACGTGGACTGGGAGCGGTTCTGGCCGCTGTTCACCTCCGCGCGACCCGCCCCGCTGCTC
TCGGACCTGCCCGAGGTGCGCGGCCTCGGCCGGGGCCTGGCCGAGGACACCGGCACCGGG
GCCGATCCCGGCGCCGCCGAGGCCCTGCGGAGCAAGCTCGCCGGGCTCTCCCCGGCCGAA
CAGGACCGGGCCCTCACCGACCTGGTCTGCGCCCACGCCGCCGCCGTCCTCGGGCACTCC
TCCGCGGGCGCCGTCGACGCCGAACGCGCCTTCAAGGACCTCGGCTTCGACTCGCTCACC
GCGGTGGGCCTGCGCAACGGCCTGGGCGCCGCCACCGGCCTCAGCCTGCCCGCCACCCTC
GTCTTCGACTACCCGACGCCCGCCGCGATGGCCGGTTACGTACGCGATCACCTGCTGGCC
GGGGCACGCCAGGAGGCGACCGCCGCCGGGGTGCAGTCGGGCCTGGACCAGCTGGAGGCG
GACCTGCTGTCCGTCGCCCTGGACAAGGACGAACGCAAGAACCTCACCCGGCGCCTCGAA
GGACTTCTGTCCCGCTTCAAGGACGCCCAGGCCGCCGCCGACGAGGAGTCGGTGAGCGGA
AAGCTCGATTCGGCTTCCGACGAAGAGATCTTCGCCTTCATCCGTGAGGAATTCGGAAGG
CCCGAGTAG

[7] KS	34..410

[7] AT	575..893

[7] methylmalonyl-CoA	766..770

[7] kr	1196..1373

[7] ACP	1485..1555

[7] KS	100..1230

[7] AT	1723..2679

[7] methylmalonyl-CoA	2296..2310

[7] kr	3586..4119

[7] ACP	4453..4665

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001227 Acyl transferase domain (Domain) G3DSA:3.40.366.10 no description IPR006162 Phosphopantetheine attachment site (PTM) PS00012 PHOSPHOPANTETHEINE IPR009081 Acyl carrier protein-like (Domain) PS50075 ACP_DOMAIN G3DSA:1.10.1200.10 no description PF00550 PP-binding SSF47336 ACP-like IPR013968 Polyketide synthase, KR (Domain) PF08659 KR IPR014030 Beta-ketoacyl synthase, N-terminal (Domain) PF00109 ketoacyl-synt IPR014031 Beta-ketoacyl synthase, C-terminal (Domain) PF02801 Ketoacyl-synt_C IPR014043 Acyl transferase (Domain) PF00698 Acyl_transf_1 IPR015083 Polyketide synthase, docking (Domain) PF08990 Docking IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain) SSF52151 FabD/lysophospholipase-like IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain) SSF55048 Probable ACP-binding domain of malonyl-CoA ACP transacylase IPR016038 Thiolase-like, subgroup (Domain) G3DSA:3.40.47.10 no description IPR016039 Thiolase-like (Domain) SSF53901 Thiolase-like IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 no description IPR020801 Polyketide synthase, acyl transferase domain (Domain) SM00827 Acyl transferase domain in polyketide syntha IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain) SM00823 Phosphopantetheine attachment site IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain) SM00825 Beta-ketoacyl synthase IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain) SM00822 no description
SignalP	No significant hit
TMHMM	No significant hit

Salino_00930 Salino_00950