Ansam_00030 Ansam_00030   Ansam_00050 Ansam_00050

Ansam_00040 : CDS information

close this sectionLocation

Organism
StrainATCC 31565
Entry nameAnsamitocin
Contig
Start / Stop / Direction2,165 / 4,411 / + [in whole cluster]
80,582 / 78,336 / - [in contig]
Location2165..4411 [in whole cluster]
complement(78336..80582) [in contig]
TypeCDS
Length2,247 bp (748 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.3 transport
Productputative ABC transporter ATP-binding/permease protein
Product (GenBank)ABC transporter
Gene
Gene (GenBank)asm4
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[12060743] The biosynthetic gene cluster of the maytansinoid antitumor agent ansamitocin from Actinosynnema pretiosum. (Proc Natl Acad Sci U S A. , 2002)
comment
Actinosynnema pretiosum ssp. auranticum ATCC 31565のcosmid libraryから、ansamitocin生合成に必要な2つのgene clustersをクローニング。

<cluster I>
Asm04(748aa) : ABC transporter(Function of homologue)

本文にコメントなし。
Related Reference
ACC
Q9L4X6
NITE
Nysta_00020
PmId
[16251298] Nystatin biosynthesis and transport: nysH and nysG genes encoding a putative ABC transporter system in Streptomyces noursei ATCC 11455 are required for efficient conversion of 10-deoxynystatin to nystatin. (Antimicrob Agents Chemother. , 2005)
comment
Blast list外 id35.08%, 1e-66, score249
Streptomyces noursei_nysG
NysG

nysG, nysHを不活化してもnystatinは細胞外へ運ばれるので、他にも代替的なtransporterがあるようだ。不活化すると10-deoxynystatinの産生量が増えるので、C-10 hydroxylation(NysL)の効率に関係がある。ATPase inhibitor sodium o-vanadateを使った実験で産生量が減少する。domainについても検討し、明らかにtype III ABC transportersに属するといっている。
Family/Domain
FD
IPR001140
comment
[IPR001140] ABC transporter, transmembrane domain (Domain)
Molecular Function:
GO:0005524 ATP binding
GO:0042626 ATPase activity, coupled to transmembrane movement of substances
FD
IPR003593, IPR003439
comment
[IPR003593] AAA+ ATPase domain (Domain)
Molecular Function:
GO:0000166 nucleotide binding
GO:0017111 nucleoside-triphosphatase activity

[IPR003439] ABC transporter-like (Domain)
Molecular Function:
GO:0005524 ATP binding
GO:0016887 ATPase activity

close this sectionSequence

selected fasta
>putative ABC transporter ATP-binding/permease protein [ABC transporter]
MTQLLDAAPTTRLTGFRVRSVVPGRQRWDVPRVRRNPRAARALEAALLRQPGITGAQASP
ATGRVLVLHDRALGVAEVGAALRDAVAAAASAPLVVEGSTTTSPPATAASTSGGTSEVAL
RPASAVLRRPLALVGVVTGVSSAVLRHAWRALLRPLRRTAAGTRLSAHPLVRALGRRRSA
LAAAAALTVACQLAELGLGLFLGWILLVLIKGEYAPLTALGVTSAPAQLLALAAGAAATC
GVVVVLSAASGARWRALGQGVRRDQRARLHRHTQRLEMRHIQRERPSRVAGVLADDVDQL
GAFFSGPAGEAVQLATSALILVPSFLVLVPGIAWIAFLPLPFVAALSLRQRDRSAADYAS
AGEQAALLRSRLSAALEAAATVKSACAEDHEAELVDLLELAGADAATRTQQHAVRFGEGI
RAWTTCSMAGTLLVGGLKVLDGTLPFERFSPLIGLPQQMVMRLTRLGPIVDQYQRTLAAH
DRVEHLMSLPTEPTGGRALARERVAGAIALDRVSFSYEGRPRVLDRLSLRAAAGRVTGIV
GATGSGKTTVAKLLMRFQDADTGRVLLDGRDVRGLRLPDLRAAVGFVAQDPFLFDGTIAD
NIRYGTFSATDEQVREAARTAEAHAFVESLPLGYDTVVGEHGAALSGGQRQRIALARTVL
KNPPVVVLDEATSAVDNETEAAIQRALATFARGRTLIVIAHRLSTVRNADHIYVLERGGV
VEQGAHEGLVASGGRYASLWELQAGARG
selected fasta
>putative ABC transporter ATP-binding/permease protein [ABC transporter]
GTGACCCAGCTCCTCGACGCCGCCCCGACCACCAGGCTCACCGGGTTCCGGGTGCGCTCG
GTCGTCCCCGGCCGCCAGCGCTGGGACGTCCCGCGCGTGCGCAGGAACCCGCGCGCCGCG
CGCGCCCTCGAAGCCGCGCTGCTCCGCCAGCCCGGCATCACCGGGGCGCAGGCCAGCCCC
GCGACCGGCCGGGTGCTGGTGCTGCACGACCGGGCGCTCGGCGTCGCCGAGGTCGGCGCC
GCCCTGCGGGACGCGGTCGCCGCCGCGGCCAGCGCCCCGCTGGTCGTCGAGGGCAGCACC
ACGACGAGCCCACCGGCCACCGCCGCGAGCACGTCCGGCGGCACGTCCGAGGTCGCGCTC
CGGCCCGCGAGCGCCGTCCTGCGCCGCCCGCTCGCCCTGGTCGGCGTGGTCACCGGCGTC
TCGTCCGCCGTCCTGCGCCACGCCTGGCGCGCCCTCCTGCGGCCCCTGCGCCGCACCGCC
GCGGGCACCCGGCTCTCCGCCCACCCGCTGGTGCGCGCCCTCGGCCGCCGCCGGTCGGCG
CTCGCCGCCGCCGCCGCGCTGACGGTGGCCTGCCAGCTCGCCGAGCTCGGCCTCGGGCTG
TTCCTCGGCTGGATCCTGCTGGTGCTGATCAAGGGCGAGTACGCCCCGCTCACCGCGCTG
GGCGTCACGAGCGCCCCCGCCCAGCTGCTCGCGCTCGCCGCCGGGGCCGCCGCGACCTGC
GGGGTCGTCGTCGTGCTCTCCGCCGCCTCCGGCGCCCGCTGGCGCGCGCTCGGCCAGGGC
GTCCGGCGCGACCAGCGCGCCCGCCTGCACCGCCACACCCAGCGCCTGGAGATGCGGCAC
ATCCAGCGCGAGCGGCCCAGCCGCGTCGCGGGCGTGCTCGCCGACGACGTCGACCAGCTC
GGCGCGTTCTTCTCCGGCCCGGCGGGCGAGGCGGTCCAGCTCGCCACCAGCGCGCTGATC
CTGGTCCCGTCGTTCCTGGTGCTCGTGCCCGGCATCGCCTGGATCGCGTTCCTGCCCCTG
CCGTTCGTCGCGGCCCTGTCCCTGCGCCAGCGCGACCGCTCCGCCGCCGACTACGCCAGC
GCGGGCGAGCAGGCCGCCCTGCTGCGCAGCAGGCTCTCCGCCGCACTGGAGGCCGCCGCC
ACGGTCAAGAGCGCCTGCGCCGAGGACCACGAGGCCGAGCTGGTCGACCTGCTGGAGCTG
GCGGGCGCCGACGCGGCCACGCGCACCCAGCAGCACGCCGTCCGGTTCGGCGAGGGCATC
CGCGCCTGGACCACCTGCTCCATGGCGGGCACCCTGCTCGTGGGCGGGCTCAAGGTGCTC
GACGGCACGCTCCCGTTCGAGCGGTTCAGCCCGCTGATCGGGCTGCCGCAGCAGATGGTC
ATGCGGCTGACCCGGCTGGGCCCGATCGTCGACCAGTACCAGCGCACCCTCGCCGCGCAC
GACCGGGTCGAGCACCTGATGTCCCTGCCCACCGAGCCCACCGGTGGCCGCGCGCTGGCC
CGCGAGCGGGTCGCGGGCGCCATCGCGCTCGACCGCGTGTCCTTCTCCTACGAGGGCCGC
CCGCGCGTGCTGGACCGGTTGTCGCTGCGCGCGGCGGCGGGCCGGGTCACCGGCATCGTC
GGCGCGACCGGCTCGGGCAAGACCACCGTCGCCAAGCTGCTGATGCGCTTCCAGGACGCG
GACACCGGCCGGGTGCTGCTCGACGGGCGGGACGTGCGCGGCCTGCGCCTGCCCGACCTG
CGCGCGGCCGTCGGGTTCGTCGCCCAGGACCCGTTCCTGTTCGACGGCACGATCGCGGAC
AACATCCGCTACGGCACGTTCTCCGCCACCGACGAGCAGGTCCGCGAGGCCGCCCGCACG
GCCGAGGCCCACGCGTTCGTCGAGTCGCTGCCCCTGGGCTACGACACGGTGGTCGGCGAG
CACGGCGCGGCGCTGTCCGGCGGGCAGCGGCAGCGGATCGCGCTCGCCAGGACCGTGCTG
AAGAACCCGCCCGTGGTGGTCCTGGACGAGGCCACGTCGGCGGTGGACAACGAGACCGAG
GCGGCGATCCAGCGCGCGCTGGCGACGTTCGCGCGGGGCCGCACCCTGATCGTGATCGCG
CACCGGCTCTCCACCGTCCGCAACGCGGACCACATCTACGTGCTGGAGCGGGGCGGCGTC
GTCGAGCAGGGCGCGCACGAGGGCCTGGTGGCCTCCGGCGGGCGCTACGCCTCGCTGTGG
GAGCTCCAGGCGGGGGCGAGGGGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001140 ABC transporter, transmembrane domain (Domain)
[207-462] 2.1e-06 PF00664
PF00664   ABC_membrane
IPR003439 ABC transporter-like (Domain)
[508-742] PS50893
PS50893   ABC_TRANSPORTER_2
[548-673] 6.1e-21 PF00005
PF00005   ABC_tran
IPR003593 AAA+ ATPase domain (Domain)
[533-719] 3.89999861795218e-18 SM00382
SM00382   AAA
IPR011527 ABC transporter, transmembrane domain, type 1 (Domain)
[169-493] 7.0999832511256e-38 SSF90123
SSF90123   ABC_TM_1
IPR017871 ABC transporter, conserved site (Conserved_site)
[645-659] PS00211
PS00211   ABC_TRANSPORTER_1
IPR017940 ABC transporter, integral membrane type 1 (Domain)
[182-475] PS50929
PS50929   ABC_TM1F
SignalP No significant hit
TMHMM
[185-207] Transmembrane (i-o) [227-249] Transmembrane (o-i) [318-340] Transmembrane (i-o)
Transmembrane 3   
Ansam_00030 Ansam_00030   Ansam_00050 Ansam_00050
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