Ansam_00040 Ansam_00040   Ansam_00060 Ansam_00060

Ansam_00050 : CDS information

close this sectionLocation

Organism
StrainATCC 31565
Entry nameAnsamitocin
Contig
Start / Stop / Direction4,420 / 5,670 / + [in whole cluster]
78,327 / 77,077 / - [in contig]
Location4420..5670 [in whole cluster]
complement(77077..78327) [in contig]
TypeCDS
Length1,251 bp (416 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.3 transport
Productputative transporter
Product (GenBank)Na+/H+ antiporter
Gene
Gene (GenBank)asm5
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[12060743] The biosynthetic gene cluster of the maytansinoid antitumor agent ansamitocin from Actinosynnema pretiosum. (Proc Natl Acad Sci U S A. , 2002)
comment
Actinosynnema pretiosum ssp. auranticum ATCC 31565のcosmid libraryから、ansamitocin生合成に必要な2つのgene clustersをクローニング。

<cluster I>
Asm05(416aa) : Na/H antiporter(Function of homologue)

本文にコメントなし。
Related Reference
ACC
Q6BJU7
PmId
comment
BLAST id34%, C末400aaほど余る
Debaryomyces hansenii(Yeast)_KHA1
Putative sodium KHA1 transporter

Debaryomyces hansenii kha1の機能解析。
アミノ酸配列はNa+/H+ transporterのクラスに属した。また、Na(+)に対して高い耐性を示すが、他のtoxic cationsあるいはstress factorsには耐性を示さないことを確認している。しかし、機能を同定するにはまだ実験が不十分であるとも言っている。現時点ではputative Na+ transporter。
Family/Domain
FD
IPR006153
comment
[IPR006153] Cation/H+ exchanger (Domain)
Function : solute:proton antiporter activity

close this sectionSequence

selected fasta
>putative transporter [Na+/H+ antiporter]
MAAPAGDSLVALLFLDLAVVLALGALLGALARRLGQPSVVGEVAAGIALGPSLLGLLPGD
PVALLFPPEARPPLAFAAQLGLVLFMFLVGCELDVAELRGAGRVVGAVSAGSVVLPFCLG
LGVAALLWPQGGDALALFTAAALSITAFPVLARILAERRMQRTRSGVVALASAAVNDVVA
WCALAVVAGIVTARGPWSAVATLAWTAALVLVAVLLVRPLVGWVARTVGGSPRADAVLFA
VVVQGLLLFALATTAIGLHAVFGAFLFGAVVPKDALREAAPTLVDRVGGLSSLLLPVFFV
VAGLSVDVGGLGWSGAAEALLVLVAACVGKLVGAAGGGLLAGLPARDAAEVGVLMNARGL
TELVVLGVGLELGVLDGRLFTVLVVMALVTTAATGPLLTLIARRSGREVAALEGLR
selected fasta
>putative transporter [Na+/H+ antiporter]
GTGGCGGCCCCGGCGGGCGACTCGCTCGTCGCGCTGCTGTTCCTCGACCTCGCGGTCGTC
CTGGCGCTCGGCGCCCTGCTCGGCGCGCTGGCCAGGCGGCTGGGGCAGCCCTCGGTGGTG
GGGGAGGTCGCGGCCGGGATCGCGCTCGGCCCCAGCCTGCTCGGCCTGCTGCCCGGCGAC
CCGGTGGCGCTGCTGTTCCCGCCCGAGGCGCGCCCGCCGCTGGCGTTCGCCGCCCAGCTC
GGCCTGGTGCTGTTCATGTTCCTGGTCGGCTGCGAGCTGGACGTCGCGGAGCTGCGCGGC
GCGGGGCGGGTGGTCGGCGCGGTGTCGGCGGGCTCGGTCGTGCTGCCGTTCTGCCTCGGG
CTGGGCGTCGCGGCGCTGCTGTGGCCGCAGGGCGGTGACGCGCTCGCGCTGTTCACCGCA
GCCGCCCTGTCGATCACCGCTTTCCCCGTGCTGGCGCGGATCCTGGCCGAGCGGCGGATG
CAGCGCACCCGGTCCGGGGTGGTCGCGCTGGCGAGCGCCGCGGTGAACGACGTGGTGGCG
TGGTGCGCGCTCGCCGTCGTCGCCGGGATCGTGACCGCGCGCGGCCCCTGGTCGGCGGTG
GCCACGCTGGCCTGGACGGCGGCGCTGGTCCTGGTGGCGGTGCTCCTCGTCCGGCCGCTG
GTGGGGTGGGTTGCCCGGACGGTGGGCGGCTCGCCGCGCGCGGACGCGGTGCTGTTCGCG
GTCGTCGTCCAGGGGCTGCTGCTGTTCGCGCTCGCCACCACGGCCATCGGCCTGCACGCG
GTGTTCGGGGCGTTCCTGTTCGGGGCGGTTGTGCCCAAGGACGCGCTCCGGGAGGCCGCG
CCGACCCTGGTGGACCGGGTCGGCGGGCTCAGCTCCCTGCTGCTGCCGGTGTTCTTCGTC
GTGGCCGGGCTGTCCGTGGACGTCGGCGGCCTCGGCTGGTCCGGCGCGGCGGAGGCGCTG
CTGGTGCTCGTCGCCGCGTGCGTCGGCAAGCTGGTCGGCGCGGCGGGCGGCGGTCTGCTC
GCGGGGCTGCCCGCGCGCGACGCGGCCGAGGTCGGCGTGCTGATGAACGCCAGGGGGCTC
ACCGAGCTGGTGGTGCTGGGCGTGGGGCTGGAGCTGGGGGTGCTGGACGGGCGCCTGTTC
ACCGTCCTGGTCGTGATGGCGCTGGTGACGACGGCGGCGACCGGCCCGCTGCTGACGCTG
ATCGCCCGGCGGTCCGGGCGGGAGGTCGCCGCGCTCGAAGGACTCCGGTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR006153 Cation/H+ exchanger (Domain)
[19-402] 5.10000000000004e-64 PF00999
PF00999   Na_H_Exchanger
SignalP
[1-31] 0.98 Signal
Eukaryota   
[1-31] 0.462 Signal
Bacteria, Gram-positive   
TMHMM
[10-30] Transmembrane (o-i) [37-59] Transmembrane (i-o) [74-93] Transmembrane (o-i) [106-128] Transmembrane (i-o) [134-156] Transmembrane (o-i) [169-191] Transmembrane (i-o) [201-223] Transmembrane (o-i) [236-258] Transmembrane (i-o) [293-315] Transmembrane (o-i) [320-342] Transmembrane (i-o) [379-401] Transmembrane (o-i)
Transmembrane 11   
Ansam_00040 Ansam_00040   Ansam_00060 Ansam_00060
Page top