Ansam_00060 Ansam_00060   Ansam_00080 Ansam_00080

Ansam_00070 : CDS information

close this sectionLocation

Organism
StrainATCC 31565
Entry nameAnsamitocin
Contig
Start / Stop / Direction7,158 / 6,112 / - [in whole cluster]
75,589 / 76,635 / + [in contig]
Locationcomplement(6112..7158) [in whole cluster]
75589..76635 [in contig]
TypeCDS
Length1,047 bp (348 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.2 modification methylation
ProductC-20 O-methyltransferase
Product (GenBank)methyltransferase
Gene
Gene (GenBank)asm7
EC number2.1.1.-
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[12060743] The biosynthetic gene cluster of the maytansinoid antitumor agent ansamitocin from Actinosynnema pretiosum. (Proc Natl Acad Sci U S A. , 2002)
[14624546] The post-polyketide synthase modification steps in the biosynthesis of the antitumor agent ansamitocin by Actinosynnema pretiosum. (J Am Chem Soc. , 2003)
comment
[PMID:12060743](2002)
Actinosynnema pretiosum ssp. auranticum ATCC 31565のcosmid libraryから、ansamitocin生合成に必要な2つのgene clustersをクローニング。

<cluster I>
Asm07(348aa) : Methyltransferase

配列解析のみ。
N-methylation or C-20 O-methylationのどちらかを担うかもしれない。

---
[PMID:14624546](2003)
Actinosynnema pretiosumのansamitocin生合成gene clusterにある6genesの機能を、遺伝子不活化とmutantの化学分析によって調査。

それらはそれぞれ
halogenase (asm12),
carbamoyltransferase (asm21),
20-O-methyltransferase (asm7),
3-O-acyltransferase (asm19),
epoxidase (asm11),
N-methyltransferase (asm10)
をコードし、ansamitocin形成において6つのPKS後修飾ステップを担う。

Asm7 deletion mutantの主要産物は20-O,N-didemethyl-Ansamitocin P-3、
Asm10 deletion mutantの主要産物はN-demethyl-Ansamitocin P-3であったことから、これらmethyltransferasesの割り当てが確定している。

close this sectionSequence

selected fasta
>C-20 O-methyltransferase [methyltransferase]
MTLTDDEHTAHRHDEGAAAFQALATAAAGWGALRAAVELRLPDLLPDAPTAVPDLAALAG
VAPTRLAQLVAVLADVGVLALDERAHVRHTPLSRALRSTDGLALTRLLTAGWADEAWRGL
AEGVRTDTSPLELVHGRPLFDLLAADPAAAAVFHDAVATGPGDPFDEAIRSALDLTGAAR
VVDVGGGRGRLLAALLREHPVLSGVLFDLPEAVRDAVPELRGDDRVQIRAGDLFADRVGA
ADVYVLRTVLHVLDDAQAVRALANVRADAPAHARVVVVEALSDDEANRTYAAHSSLRLFV
LCGGRERSTAEFAELFDRAGLELRSVTPTGTPYHLLEGAPRLSPRTDG
selected fasta
>C-20 O-methyltransferase [methyltransferase]
GTGACGTTGACCGACGACGAGCACACCGCGCACCGGCACGACGAGGGCGCCGCGGCCTTC
CAGGCGCTCGCGACCGCCGCCGCAGGCTGGGGCGCGCTGCGCGCCGCCGTGGAGCTGCGC
CTGCCCGACCTGCTCCCGGACGCCCCCACCGCCGTCCCCGACCTGGCGGCGCTGGCGGGC
GTCGCGCCGACGCGGCTCGCGCAGCTGGTCGCGGTGCTCGCCGACGTGGGCGTGCTCGCG
CTGGACGAGCGCGCCCACGTCCGCCACACGCCGCTCTCCCGCGCCCTGCGCTCGACCGAC
GGCCTGGCGCTCACCCGCCTGCTCACGGCGGGCTGGGCGGACGAGGCGTGGCGCGGGCTC
GCGGAGGGCGTGCGCACCGACACCTCCCCGCTGGAGCTGGTGCACGGCCGCCCGCTGTTC
GACCTCCTCGCCGCCGACCCGGCCGCCGCCGCGGTGTTCCACGACGCGGTGGCCACCGGC
CCCGGCGACCCGTTCGACGAGGCGATCCGCTCCGCGCTCGACCTGACCGGGGCCGCGCGG
GTGGTCGACGTCGGCGGCGGGCGGGGCAGGCTGCTCGCCGCGCTGCTGCGCGAGCACCCC
GTGCTCTCGGGCGTGCTGTTCGACCTGCCCGAGGCGGTCCGGGACGCGGTTCCCGAGCTG
CGCGGCGACGACCGGGTCCAGATCAGGGCGGGCGACCTGTTCGCGGACCGGGTCGGCGCG
GCGGACGTCTACGTGCTGCGCACGGTCCTGCACGTGCTGGACGACGCGCAGGCCGTGCGG
GCGCTGGCCAACGTCCGCGCCGACGCGCCCGCGCACGCCAGGGTCGTGGTCGTGGAAGCG
CTTTCCGACGACGAGGCCAACCGGACCTACGCGGCGCACAGCAGCCTGCGGCTGTTCGTG
CTGTGCGGCGGGCGCGAGCGCTCGACGGCGGAGTTCGCCGAGCTGTTCGACCGGGCCGGG
CTGGAGCTGCGCTCGGTCACCCCGACCGGGACGCCGTACCACCTGCTGGAGGGCGCCCCA
CGCCTGAGCCCGCGCACGGACGGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001077 O-methyltransferase, family 2 (Domain)
[87-316] 9.20000000000002e-46 PF00891
PF00891   Methyltransf_2
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
[19-93] 6.80000000000001e-09 G3DSA:1.10.10.10
G3DSA:1.10.10.10   Wing_hlx_DNA_bd
IPR016461 O-methyltransferase, caffeic acid-type (Family)
[1-340] 6.59999852526376e-12 PIRSF005739
PIRSF005739   O-mtase
SignalP No significant hit
TMHMM No significant hit
Ansam_00060 Ansam_00060   Ansam_00080 Ansam_00080
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