Ansam_00070 Ansam_00070   Ansam_00090 Ansam_00090

Ansam_00080 : CDS information

close this sectionLocation

Organism
StrainATCC 31565
Entry nameAnsamitocin
Contig
Start / Stop / Direction7,359 / 10,712 / + [in whole cluster]
75,388 / 72,035 / - [in contig]
Location7359..10712 [in whole cluster]
complement(72035..75388) [in contig]
TypeCDS
Length3,354 bp (1,117 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
ProductLuxR family transcriptional activator
Product (GenBank)transcriptional regulator
Gene
Gene (GenBank)asm8
EC number
Keyword
  • AHBA
Note
Note (GenBank)
Reference
ACC
PmId
[12060743] The biosynthetic gene cluster of the maytansinoid antitumor agent ansamitocin from Actinosynnema pretiosum. (Proc Natl Acad Sci U S A. , 2002)
[23832249] Asm8, a specific LAL-type activator of 3-amino-5-hydroxybenzoate biosynthesis in ansamitocin production. (Sci China Life Sci. , 2013)
comment
[PMID:12060743](2002)
Actinosynnema pretiosum ssp. auranticum ATCC 31565のcosmid libraryから、ansamitocin生合成に必要な2つのgene clustersをクローニング。

<cluster I>
Asm08(1117aa) : Transcriptional regulator

本文にコメントなし。

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[PMID:23832249](2013)
ATCC 31565での遺伝子置換によるasm8不活化mutantはansamitocin非産生、外因性AHBAの添加で産生回復する。

asm8不活化mutantにおけるasm cluster genesの転写量をqRT-PCRでwildと比較。転写量が減少したのはすべてAHBAに関するgenesだった。(asm46も減っているが、asm46の破壊でansamitocin P3産生に影響しないことを確認している。）

よってAsm8がAHBA生合成での特異的なpositive regulatorであることが示された。
Related Reference
ACC
Q06BL5
NITE
Gelda_00100
PmId
[18214443] The LuxR family members GdmRI and GdmRII are positive regulators of geldanamycin biosynthesis in Streptomyces hygroscopicus 17997. (Arch Microbiol. , 2008)
comment
Blast 8th, id39%, 1e-93, N末側のalignment悪い
Streptomyces hygroscopicus_gdmRII
GdmRII

gdmRI, gdmRIIのいずれか一方の欠損株ではgeldanamycinの生産が完全に消失し、欠損した遺伝子をプラスミドで相補すると geldanamycin生産が回復すること、二つの遺伝子の転写は独立に行われ互いを制御していないこと、二つの遺伝子の転写はpolyketide生合成に関与する遺伝子と協調し、post-PKS modification 遺伝子である gdmNとは協調していないことが示されており、gdmRI と gdmRIIはgeldanamycin 生合成経路におけるpolyketide 生合成遺伝子特異的なpositive regulatorsであると結論付けている。

close this sectionSequence

selected fasta
>LuxR family transcriptional activator [transcriptional regulator]
MRLVDRDGQCEALAALLEGAAGGCGGAVLLRGAPGHGKTALLGRLVVMAEGAGFRCLVVE
ADALPEVAEVAAGGGALLVAVDDAGGADVGALFDLVAWARRTTAVLALTDLPRLPDDDPL
GAVARTPGALVLDLPPLRPETTAALLGGSPWPVTGGNPALVRGLAHDLVAGEAGPGAAYR
AALAGVLDRCGAEVAAVAHATAVLTGPAVGLLTAPAAALPAGLRAAPTAALLTAPAAVLP
GGVLPGAALPDGAHPAAGPGEACEGTAPAGEALSAAVRPGEACPGTALPDGVLPGVASPG
GAFPAADVLGGPFPAAVRSAAALPDWALPAAVRPGEACPGTALPTDAPPGAAPPGGALPD
GGARPLTSGEVGVRQGGVGPVAPGRAGASPGGAEPLVPGPVSGVRADPAGGDGASAFGRV
AADHVVPAGVRPRASSDPAAPVDLVADLLGRAPASVVDARARLAGAGLDRADPAAVLDVL
PAARRAELHLRAARTLHERGESPAVVADHLVTAGLPAPAWAVDVLVAAADHALAADDVPT
AVARLAAAHRRDDADQPRAVPPRAVPDPPVAAHRCGCADRTAAPAERRTSPEAAAVAGPA
LTARLARARWQLNPAAALCHLDPLADALAADPDALAPRDAAALVRQLVWHGRTTAAGRVL
AALRAREHAAELPDLETWLSLACPPLTASRRPTTLHASAPHTSAPRTSAHPTPLAPPPSR
VDPWLREAASLVGGFALGDQRRAAAEAAHYLRHAHLSHTSPWSEEPALLALLALVRADRA
ADADRACARLLDEARARRAPTWVASFAAAASVIALRLGDLAGAAGHARAALDAVPSHGWG
ASVGLPLGSLILADTLRGRRREAGRHVTAPVPDALFDGPAAPHYLHARGHHHLASGRDHA
ALADFLACGDLLRSWGLDLPGLAPWRTGAAEAWTRLGNHDRARRLVFDQLATTGADAGRP
RALALRALAPLSPPTRRPHLLAEAADALERCGDRYELAATLACLGAAHHALGDHRRARAV
LRRAWHLARACGARPLCARLEPDAADPVEHDTSGLTGSERKVAALAVTGLTNRQIAEKLF
VTPSTVEQHLTRVFRKLRVTHRADLPTTLHADLTTPA
selected fasta
>LuxR family transcriptional activator [transcriptional regulator]
ATGCGGCTGGTGGACCGCGACGGGCAGTGCGAGGCGCTGGCTGCCCTGCTGGAGGGCGCG
GCGGGCGGGTGCGGGGGCGCGGTGCTGCTCAGGGGCGCGCCGGGGCACGGGAAGACCGCG
CTGCTGGGGCGCCTGGTCGTGATGGCCGAGGGCGCGGGGTTCCGGTGCCTGGTGGTCGAG
GCGGACGCGCTGCCCGAGGTCGCGGAGGTCGCGGCGGGCGGCGGCGCGCTGCTGGTGGCC
GTGGACGACGCCGGGGGCGCCGACGTGGGCGCGCTGTTCGACCTGGTGGCGTGGGCGAGA
CGCACGACCGCCGTCCTCGCGCTCACCGACCTGCCCCGCCTGCCCGACGACGACCCGCTG
GGCGCCGTGGCGCGCACGCCGGGCGCCCTCGTGCTCGACCTCCCGCCACTGCGACCGGAG
ACCACCGCCGCGCTGCTCGGCGGGTCCCCGTGGCCGGTGACCGGGGGGAACCCCGCGCTG
GTGCGCGGGCTGGCCCACGACCTCGTGGCGGGGGAGGCGGGACCGGGGGCCGCCTACCGC
GCCGCCCTCGCCGGGGTGCTCGACCGCTGCGGCGCCGAGGTGGCCGCCGTCGCGCACGCC
ACCGCCGTGCTCACCGGGCCCGCCGTTGGGCTTCTCACCGCACCCGCCGCCGCGCTTCCC
GCCGGGCTTCGCGCCGCACCCACCGCCGCGCTTCTCACCGCACCCGCCGCCGTGCTTCCC
GGCGGGGTGCTTCCTGGTGCCGCGCTTCCTGACGGGGCGCACCCTGCTGCGGGCCCTGGT
GAGGCGTGCGAAGGCACCGCGCCTGCTGGCGAGGCTCTTTCCGCTGCTGTGCGCCCTGGC
GAGGCGTGCCCAGGCACCGCGCTTCCCGACGGGGTGCTTCCGGGGGTCGCGTCTCCCGGC
GGGGCGTTCCCCGCCGCCGACGTTCTCGGCGGGCCCTTCCCCGCCGCCGTGCGTTCCGCC
GCCGCGCTTCCAGACTGGGCCCTCCCCGCCGCCGTGCGTCCCGGCGAGGCGTGCCCTGGC
ACCGCGCTTCCCACCGACGCGCCCCCTGGGGCCGCGCCCCCTGGCGGGGCGCTTCCCGAC
GGCGGGGCGCGACCGCTCACGTCTGGCGAGGTGGGCGTCCGGCAGGGGGGAGTGGGGCCG
GTCGCGCCCGGTCGCGCCGGTGCCTCGCCGGGGGGCGCTGAGCCGCTCGTGCCCGGCCCG
GTGTCTGGGGTGAGGGCTGATCCCGCCGGGGGGGACGGGGCTTCCGCGTTCGGTCGGGTG
GCTGCGGATCACGTCGTTCCCGCTGGGGTCCGCCCCCGCGCGTCCTCGGACCCCGCCGCG
CCCGTCGACCTGGTCGCCGACCTGCTCGGTCGGGCCCCCGCCTCCGTCGTCGACGCCCGC
GCCCGCCTCGCGGGAGCCGGGCTCGACCGGGCCGATCCCGCCGCTGTGCTGGACGTGCTG
CCCGCCGCCCGGCGCGCCGAGCTGCACCTGCGCGCCGCGCGGACGTTGCACGAGCGCGGT
GAGTCGCCCGCCGTCGTCGCCGACCACCTCGTCACCGCCGGGCTGCCCGCGCCCGCCTGG
GCCGTCGACGTGCTCGTCGCCGCCGCCGACCACGCGCTCGCCGCCGACGACGTGCCCACC
GCCGTCGCACGGCTCGCCGCCGCCCACCGCCGGGACGACGCGGACCAACCGCGGGCCGTC
CCCCCGCGCGCAGTCCCGGACCCGCCCGTCGCCGCCCACCGGTGCGGGTGCGCGGACCGG
ACGGCCGCGCCCGCCGAGCGCCGCACGTCGCCCGAGGCCGCCGCCGTCGCCGGGCCCGCG
CTCACCGCCCGGCTCGCCCGCGCGCGCTGGCAGCTCAACCCGGCTGCCGCGCTGTGCCAC
CTCGACCCGCTCGCCGACGCGCTCGCCGCCGACCCCGACGCGCTCGCCCCGCGGGACGCC
GCCGCGCTGGTGCGCCAGCTGGTCTGGCACGGGCGCACCACCGCCGCCGGGCGCGTGCTC
GCCGCGCTGCGCGCCCGCGAGCACGCCGCCGAGCTGCCCGACCTGGAGACCTGGCTCTCC
CTCGCCTGCCCGCCGCTCACCGCCTCCCGCCGCCCCACAACCCTCCACGCCTCCGCCCCG
CACACCTCCGCCCCGCGCACCTCGGCCCACCCCACCCCCCTCGCCCCACCCCCGAGCCGC
GTCGACCCGTGGTTGCGCGAGGCCGCCAGCCTGGTCGGCGGGTTCGCGCTCGGGGACCAG
CGCCGGGCCGCCGCCGAGGCCGCGCACTACCTGCGCCACGCCCACCTCAGCCACACCTCC
CCGTGGTCCGAGGAACCCGCGCTGCTCGCCCTGCTAGCCCTGGTGCGCGCCGACCGCGCC
GCCGACGCCGACCGGGCCTGCGCCCGGCTGCTCGACGAGGCCCGCGCCCGGCGCGCGCCC
ACCTGGGTCGCCTCCTTCGCCGCCGCCGCCTCGGTGATCGCGCTGCGGCTCGGCGACCTC
GCGGGCGCCGCCGGGCACGCCCGCGCCGCGCTCGACGCCGTCCCCTCGCACGGCTGGGGC
GCGTCCGTGGGCCTGCCGCTGGGCTCGCTGATCCTCGCCGACACCCTGCGCGGGCGGCGC
CGGGAGGCCGGGCGGCACGTCACCGCGCCCGTGCCGGACGCCCTCTTCGACGGCCCCGCC
GCCCCGCACTACCTGCACGCGCGCGGCCACCACCACCTCGCGTCCGGGCGGGACCACGCG
GCGCTCGCGGACTTCCTGGCGTGCGGCGACCTCCTGCGCTCGTGGGGCCTCGACCTGCCC
GGCCTCGCGCCGTGGCGCACCGGGGCCGCCGAGGCGTGGACCCGCCTGGGCAACCACGAC
CGCGCCCGGCGGCTGGTGTTCGACCAGCTCGCCACGACCGGCGCCGACGCGGGCCGCCCC
AGGGCGCTGGCGCTGCGCGCGCTCGCCCCGCTCAGCCCGCCGACCCGCCGCCCGCACCTG
CTCGCCGAGGCCGCCGACGCCCTGGAGCGCTGCGGTGACCGCTACGAGCTGGCCGCCACC
CTCGCCTGCCTCGGCGCCGCGCACCACGCCCTCGGCGACCACCGCCGCGCCCGCGCCGTG
CTGCGCAGGGCCTGGCACCTGGCCAGGGCGTGTGGCGCGCGACCGCTGTGCGCGCGCCTG
GAACCGGACGCGGCCGACCCCGTCGAGCACGACACCAGCGGCCTCACCGGCTCCGAGCGC
AAGGTCGCCGCCCTGGCCGTCACCGGCCTCACCAACCGGCAGATCGCCGAGAAGCTGTTC
GTCACCCCGAGCACCGTCGAGCAGCACCTGACCAGGGTGTTCCGCAAGCTGCGCGTCACC
CACCGCGCCGACCTGCCCACCACCCTGCACGCCGACCTGACCACCCCCGCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000792 Transcription regulator LuxR, C-terminal (Domain)
[1055-1069] 7.99999145876753e-09 PR00038 [1069-1085] 7.99999145876753e-09 PR00038 [1085-1097] 7.99999145876753e-09 PR00038
PR00038   HTHLUXR
[1069-1096] PS00622
PS00622   HTH_LUXR_1
[1054-1105] 2e-13 PF00196
PF00196   GerE
[1052-1109] 3.49999466863949e-21 SM00421
SM00421   HTH_LUXR
[1048-1113] PS50043
PS50043   HTH_LUXR_2
IPR011990 Tetratricopeptide-like helical (Domain)
[745-1062] 2e-11 G3DSA:1.25.40.10
G3DSA:1.25.40.10   TPR-like_helical
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
[1063-1105] 1.3e-17 G3DSA:1.10.10.10
G3DSA:1.10.10.10   Wing_hlx_DNA_bd
IPR016032 Signal transduction response regulator, C-terminal effector (Domain)
[1033-1111] 1.39999892049878e-14 SSF46894
SSF46894   Bipartite_resp_reg_C-effector
SignalP No significant hit
TMHMM No significant hit
Ansam_00070 Ansam_00070   Ansam_00090 Ansam_00090
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