Ansam_00080 Ansam_00080   Ansam_00100 Ansam_00100

Ansam_00090 : CDS information

close this sectionLocation

Organism
StrainATCC 31565
Entry nameAnsamitocin
Contig
Start / Stop / Direction10,768 / 15,537 / + [in whole cluster]
71,979 / 67,210 / - [in contig]
Location10768..15537 [in whole cluster]
complement(67210..71979) [in contig]
TypeCDS
Length4,770 bp (1,589 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)polyketide synthase
Gene
Gene (GenBank)asmC
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[12060743] The biosynthetic gene cluster of the maytansinoid antitumor agent ansamitocin from Actinosynnema pretiosum. (Proc Natl Acad Sci U S A. , 2002)
comment
Actinosynnema pretiosum ssp. auranticum ATCC 31565のcosmid libraryから、ansamitocin生合成に必要な2つのgene clustersをクローニング。

<cluster I>
AsmC(1589aa, 164.6 kDa) Polyketide synthase
Module 5 : KS AT KR ACP

close this sectionPKS/NRPS Module

5 methylmalonyl-CoA
KS36..410
AT558..861
KR1143..1323
ACP1424..1494

close this sectionSequence

selected fasta
>polyketide synthase [polyketide synthase]
MTDGTHEKLVDYLRWVTADLHETRRKLADLESAGHEPIAITGLACRAPGGVRTPEALWEL
VDGGVDAITGFPTDRGWDLAGLYDPDPTRPGTCYTREGGFLHDAAEFDAGFFGIGPREAV
TIDPQHRMLLETSWEAVERAGLDPLSLRGTGTGVFSGVVYQNYGNRRRVAEEFEGHLAIG
SSGSVASGRVAYALGLHGPAITVDTACSSSLVAIHLAAQSLRRGECAMALAGGATVMPLS
DVFVEFARQRGLSPDGRCKAFSADADGTAWGEGVGVVLLERLSDARRAGRPVLAVIRGSA
VNSDGASNGLTAPNGAAQQRVIEAALEDAGLRPSDVDLLEAHGTGTRLGDPIEAAALQAT
YGRARPEGRPLLLGSLKSNIGHAQGAAGVLGLVKVVQALRHGVAPRTLHADRLTPEVDWT
SGALRVLTEPAPWPRGERPRRAAVSAFGMSGTNAHLVVEEGDAPSAPGERRPAGALPLLL
SARGGAALRAAAADLVELPGEPVDVCWSLLTGRARLVDRAVVVAADRAGFTAGLEALAAD
RPSAAAVTGRADVAGRTVLVFPGQGGQWVGMGARLLDESPVFAAAVARCAAAVEPLVDFR
VVDALRGGDLGRVDVVQPVSFVLMVALARLWRSVGVEPDVVVGHSQGEIAAACVAGALSL
EDAARVVVLRSRAIAAEAGGGGMASIPLPRAEVERLITGTGVSVAAVNGPGSVVVSGDVD
PLVARVEGARRLPVDYASHSPAVDVLRGRLLADLARVTPRPAAIPMLSTVTGKWLDGAEL
DADYWFANLREPVRFADAVTELAGADCAAFVEVGAHPVLTAAIQDVLGERTAVVTGTLRR
DDGGLDRFLRSAAALHVRGVPVDWAPVFEGLDARRADLPTYPFQRARHWLQEAEPDARQA
GGDDPLWAAARDADGLCAELELTEPDQRAALRAVLPALTAWHERRSERALLDSWRYRITW
RTLPEPPESAPRGDWLVVGPHPDLLADLLPGAALDPGDADRAALADLLRPHTAVAGVLAT
PGADVLTLVQALGDAGATAPLWCATTGAVAISPHDGPPDPAQAATWGLGRVVALEHPGRW
GGLVDLADPADPRQRRGLAAVLAGPGVDDGGEDQVAIRAQGVFARRLVRAEPQAPAAPWT
PRGTVLITGGTGGVAAHVARRLAALGADHLVLTSRRGPSAPGAPALAADLEALGARVTTA
ACDVADRAALAALLTGLERAGEQVTAVVHAAGANAQTPVADTTPEEHARVQAAKALGAEH
LDELLGGRPLDAFVLFSSNAGVWGSGGQCAYAAANARLDALAQRRRAHGRVATSVAWGAW
DGGGMSSATPDIAAQLAQAGLRLMPPDLAVSALLDAVARDESTTVVTDVRWEAFAARFTA
LRRSPLLADLPEARPEPVAATAEPDRGLAGELAALPAPERLRRLTDLVRAHAAAALGHPG
PESVPADVAFRDLGVDSLAAVELRNRACAATGAKLTPTAVFDHPTPRALAEHLAEHLAAA
RDSAAPDAAPDAEDPTSALRDLDALAAALPAAAGDDALRERVTARLREVLGRWEELTGGP
GGPDDLDLDHISDDELFRLADSRLGPTGA
selected fasta
>polyketide synthase [polyketide synthase]
ATGACCGACGGGACCCACGAGAAGCTGGTCGACTACCTCCGGTGGGTCACCGCCGACCTG
CACGAGACCCGGCGCAAGCTCGCCGACCTGGAGTCGGCGGGCCACGAGCCGATCGCGATC
ACCGGCCTGGCCTGCCGCGCCCCCGGCGGCGTCCGCACCCCGGAAGCGCTGTGGGAGCTG
GTGGACGGCGGCGTCGACGCGATCACCGGCTTCCCCACCGACCGGGGCTGGGACCTGGCC
GGGCTCTACGACCCCGACCCGACCAGGCCGGGCACCTGCTACACCCGCGAGGGCGGGTTC
CTGCACGACGCCGCCGAGTTCGACGCCGGGTTCTTCGGCATCGGCCCGCGCGAGGCCGTC
ACGATCGACCCGCAGCACCGGATGCTCCTGGAGACCTCCTGGGAGGCGGTCGAGCGCGCG
GGCCTCGACCCGCTGTCGCTGCGCGGCACCGGAACCGGCGTCTTCTCCGGCGTGGTCTAC
CAGAACTACGGCAACCGGCGGCGCGTCGCCGAGGAGTTCGAGGGCCACCTGGCCATCGGC
AGCTCCGGCAGCGTCGCCTCCGGCCGGGTCGCCTACGCGCTCGGCCTGCACGGGCCCGCG
ATCACCGTGGACACCGCCTGCTCGTCGTCGCTGGTCGCGATCCACCTGGCCGCGCAGTCG
CTGCGGCGCGGCGAGTGCGCGATGGCGCTGGCGGGCGGCGCGACGGTCATGCCCCTGTCG
GACGTGTTCGTGGAGTTCGCCCGGCAGCGCGGACTGTCCCCGGACGGGCGCTGCAAGGCG
TTCTCCGCCGACGCCGACGGCACGGCGTGGGGTGAGGGCGTCGGCGTGGTGCTGCTGGAG
CGGCTCTCGGACGCGCGCCGCGCCGGGAGGCCGGTGCTGGCGGTGATCCGGGGCAGCGCG
GTCAACTCCGACGGCGCGTCCAACGGGCTGACCGCGCCCAACGGGGCCGCGCAGCAGCGC
GTCATCGAGGCGGCGCTGGAGGACGCGGGGCTGCGGCCGTCCGACGTGGACCTGCTGGAG
GCGCACGGCACCGGCACCCGCCTCGGCGACCCGATCGAGGCGGCGGCGCTCCAGGCCACC
TACGGGCGCGCCAGGCCCGAGGGCAGGCCGCTGCTGCTCGGCTCGCTCAAGTCCAACATC
GGGCACGCCCAGGGCGCGGCCGGGGTGCTGGGCCTGGTCAAGGTCGTGCAGGCGCTGCGC
CACGGCGTCGCGCCGCGCACCCTGCACGCCGACCGGCTCACCCCCGAGGTCGACTGGACC
TCCGGCGCGCTGCGCGTGCTGACCGAACCCGCGCCGTGGCCGCGCGGGGAGCGGCCCCGG
CGCGCGGCGGTGTCCGCGTTCGGCATGAGCGGGACCAACGCGCACCTGGTCGTGGAGGAG
GGCGACGCGCCGTCCGCGCCGGGGGAGCGGCGTCCGGCGGGCGCGCTGCCGCTGCTGCTG
TCCGCGCGCGGGGGCGCGGCGCTGCGCGCGGCGGCGGCCGACCTGGTCGAGCTGCCGGGG
GAGCCGGTGGACGTGTGCTGGTCGCTGCTCACCGGCCGCGCCCGGCTGGTCGACCGCGCG
GTGGTGGTCGCCGCCGACCGGGCCGGGTTCACCGCCGGGCTGGAGGCGCTGGCCGCCGAC
CGGCCCTCGGCCGCCGCGGTGACCGGGCGGGCCGACGTGGCGGGCCGCACGGTGCTCGTG
TTCCCCGGCCAGGGCGGCCAGTGGGTGGGCATGGGCGCCCGGCTGCTCGACGAGTCGCCG
GTGTTCGCGGCGGCCGTGGCGCGCTGCGCGGCGGCCGTGGAGCCGCTGGTGGACTTCCGC
GTGGTGGACGCGCTGCGCGGCGGCGACCTCGGCCGGGTGGACGTGGTGCAGCCGGTGTCG
TTCGTGCTGATGGTGGCGCTGGCGCGGCTGTGGCGCTCGGTCGGCGTCGAGCCGGACGTG
GTCGTCGGCCACTCGCAGGGCGAGATCGCGGCGGCCTGCGTGGCCGGGGCGCTCTCGCTG
GAGGACGCGGCGCGGGTGGTGGTGCTGCGCAGCCGGGCCATCGCCGCCGAGGCGGGCGGC
GGCGGCATGGCCTCGATCCCGCTGCCGCGCGCGGAGGTCGAACGCCTGATCACCGGCACC
GGGGTGTCCGTGGCGGCGGTCAACGGACCCGGATCGGTGGTCGTCTCCGGCGACGTCGAC
CCGCTCGTGGCGCGGGTGGAGGGCGCGCGCAGGCTGCCGGTGGACTACGCCTCGCACTCG
CCCGCCGTCGACGTGCTGCGCGGGCGGCTGCTGGCCGACCTCGCGCGGGTCACGCCCCGA
CCGGCCGCGATCCCGATGCTGTCCACCGTCACGGGGAAGTGGCTGGACGGCGCCGAGCTG
GACGCCGACTACTGGTTCGCCAACCTGCGCGAGCCGGTCCGCTTCGCCGACGCGGTCACC
GAGCTCGCCGGGGCGGACTGCGCGGCGTTCGTGGAGGTCGGGGCGCACCCGGTGCTCACC
GCCGCGATCCAGGACGTCCTGGGCGAGCGGACCGCCGTGGTCACCGGCACGCTGCGCCGC
GACGACGGCGGCCTCGACCGGTTCCTGCGCTCCGCCGCCGCGCTGCACGTGCGCGGCGTG
CCCGTCGACTGGGCCCCCGTCTTCGAGGGCCTGGACGCCCGGCGCGCCGACCTGCCCACC
TACCCGTTCCAGCGCGCCCGCCACTGGCTCCAGGAGGCCGAACCCGACGCCCGGCAGGCG
GGCGGCGACGACCCGCTGTGGGCGGCGGCGCGCGACGCCGACGGCCTGTGCGCCGAGCTG
GAGCTCACCGAGCCCGACCAGCGCGCCGCGCTGCGCGCCGTCCTGCCCGCGCTGACCGCC
TGGCACGAGCGCAGGAGCGAGCGGGCCCTGCTGGACTCCTGGCGCTACCGGATCACCTGG
CGCACCCTGCCCGAACCGCCCGAGTCCGCCCCGCGCGGCGACTGGCTCGTCGTCGGACCG
CACCCCGACCTGCTGGCCGACCTGCTGCCCGGCGCCGCGCTCGACCCCGGCGACGCCGAC
CGCGCCGCCCTCGCCGACCTGCTCCGCCCGCACACCGCCGTCGCGGGCGTCCTTGCCACG
CCGGGCGCCGACGTGCTCACCCTGGTCCAGGCGCTCGGCGACGCGGGCGCCACCGCGCCG
CTGTGGTGCGCCACCACCGGGGCCGTCGCGATCTCCCCGCACGACGGCCCGCCGGACCCC
GCCCAGGCCGCCACCTGGGGACTGGGCCGCGTCGTCGCGCTGGAGCACCCCGGCCGCTGG
GGCGGGCTGGTCGACCTCGCCGACCCCGCCGACCCGCGCCAGCGCCGCGGGCTCGCCGCC
GTCCTCGCCGGACCCGGCGTCGACGACGGAGGTGAGGACCAGGTCGCCATCCGCGCGCAG
GGCGTGTTCGCGCGCAGGCTCGTGCGCGCCGAACCGCAGGCCCCGGCCGCCCCGTGGACC
CCGCGCGGCACGGTCCTGATCACCGGCGGAACCGGCGGCGTCGCCGCGCACGTGGCCCGC
CGCCTCGCCGCCCTCGGCGCCGACCACCTCGTCCTCACCAGCCGCCGCGGCCCCTCGGCC
CCCGGCGCGCCCGCGCTCGCCGCCGACCTGGAGGCGCTGGGCGCCCGCGTCACCACCGCC
GCCTGCGACGTCGCCGACCGCGCCGCGCTCGCCGCCCTGCTGACCGGCCTGGAGCGCGCG
GGCGAGCAGGTCACCGCCGTCGTGCACGCGGCGGGCGCCAACGCCCAGACCCCCGTCGCC
GACACCACCCCCGAGGAGCACGCGCGCGTCCAGGCCGCCAAGGCGCTCGGCGCCGAGCAC
CTCGACGAGCTGCTGGGCGGGCGCCCGCTCGACGCGTTCGTCCTGTTCTCCTCCAACGCG
GGCGTGTGGGGCAGCGGCGGCCAGTGCGCCTACGCCGCCGCCAACGCCCGCCTCGACGCA
CTGGCCCAGCGCCGCCGCGCGCACGGCCGCGTCGCCACCTCCGTCGCCTGGGGCGCCTGG
GACGGCGGCGGCATGTCCAGCGCCACCCCGGACATCGCCGCCCAGCTCGCCCAGGCTGGC
CTGCGCCTGATGCCCCCGGACCTGGCGGTGTCCGCGCTGCTCGACGCCGTCGCCCGCGAC
GAGAGCACCACCGTCGTGACCGACGTGCGCTGGGAGGCGTTCGCCGCCCGGTTCACCGCC
CTGCGCCGCAGCCCCCTGCTCGCCGACCTGCCCGAGGCCCGCCCCGAACCGGTCGCCGCC
ACCGCCGAGCCCGACCGGGGGCTGGCGGGCGAGCTGGCCGCGCTGCCCGCGCCCGAGCGG
CTGCGCAGGCTCACCGACCTGGTGCGCGCGCACGCCGCCGCCGCGCTCGGCCACCCCGGA
CCCGAGTCGGTGCCCGCCGACGTGGCCTTCCGCGACCTCGGCGTCGACTCGCTCGCCGCC
GTCGAGCTGCGCAACCGGGCCTGCGCCGCGACCGGCGCGAAGCTCACGCCCACCGCGGTC
TTCGACCACCCCACCCCGCGCGCGCTCGCCGAGCACCTGGCCGAGCACCTCGCCGCCGCC
AGGGACAGCGCCGCCCCCGACGCGGCCCCGGACGCCGAGGACCCCACGTCCGCGCTGCGC
GACCTGGACGCCCTGGCCGCCGCCCTGCCCGCCGCCGCGGGCGACGACGCGCTGCGCGAG
CGGGTCACCGCGCGCCTGCGCGAGGTCCTGGGCCGCTGGGAGGAGCTGACCGGCGGACCC
GGCGGACCCGACGACCTCGATCTCGACCACATCAGCGACGACGAGCTGTTCCGCCTGGCC
GACAGCAGGCTCGGCCCGACCGGCGCCTGA
[5] KS36..410
[5] AT558..861
[5] methylmalonyl-CoA736..740
[5] KR1143..1323
[5] ACP1424..1494
[5] KS106..1230
[5] AT1672..2583
[5] methylmalonyl-CoA2206..2220
[5] KR3427..3969
[5] ACP4270..4482

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001227 Acyl transferase domain (Domain)
[551-677] 1.10000000000001e-71 G3DSA:3.40.366.10 [736-851] 1.10000000000001e-71 G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
[1143-1309] 1.2e-54 PF00106
PF00106   adh_short
IPR006162 Phosphopantetheine attachment site (PTM)
[1452-1467] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[1423-1498] 3.8e-22 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[1424-1494] PS50075
PS50075   ACP_DOMAIN
[1416-1511] 3.1999989904635e-24 SSF47336
SSF47336   ACP_like
[1427-1493] 2.3e-13 PF00550
PF00550   PP-binding
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[36-285] 6.99999999999989e-92 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[293-410] 4.30000000000003e-47 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
[558-861] 2.90000000000003e-93 PF00698
PF00698   Acyl_transf_1
IPR015083 Polyketide synthase, docking (Domain)
[5-29] 3.7e-11 PF08990
PF08990   Docking
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
[556-845] 1.50000965748778e-67 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
[679-735] 3.89999861795218e-13 SSF55048
SSF55048   Malonyl_transacylase_ACP-bd
IPR016038 Thiolase-like, subgroup (Domain)
[38-297] 2.90000000000003e-87 G3DSA:3.40.47.10 [298-461] 3.29999999999997e-64 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[28-409] 6.19996040581186e-102 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
[1143-1362] 6.59999999999996e-38 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[198-214] PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
[560-842] 1.09999184471405e-109 SM00827
SM00827   PKS_AT
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
[1425-1497] 2.1000026783403e-34 SM00823
SM00823   PKS_PP
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
[38-463] SM00825
SM00825   PKS_KS
IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain)
[1143-1323] 1.10000150671643e-48 SM00822
SM00822   PKS_KR
SignalP No significant hit
TMHMM No significant hit
Ansam_00080 Ansam_00080   Ansam_00100 Ansam_00100
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