Ansam_00130 : CDS information

Location

Organism	Actinosynnema pretiosum subsp. auranticum
Strain	ATCC 31565
Entry name	Ansamitocin
Contig	AF453501
Start / Stop / Direction	28,984 / 27,434 / - [in whole cluster] 53,763 / 55,313 / + [in contig]
Location	complement(27434..28984) [in whole cluster] 53763..55313 [in contig]
Type	CDS
Length	1,551 bp (516 aa)

Annotation

Category	3.3 modification reduction
Product	epoxidase
Product (GenBank)	oxygenase
Gene
Gene (GenBank)	asm11
EC number
Keyword
Note	The N-terminal position was modified from original INSDC entry.
Note (GenBank)
Reference	ACC Q8KUF9 PmId [12060743] The biosynthetic gene cluster of the maytansinoid antitumor agent ansamitocin from Actinosynnema pretiosum. (Proc Natl Acad Sci U S A. , 2002) [14624546] The post-polyketide synthase modification steps in the biosynthesis of the antitumor agent ansamitocin by Actinosynnema pretiosum. (J Am Chem Soc. , 2003) comment [PMID:12060743](2002) Actinosynnema pretiosum ssp. auranticum ATCC 31565のcosmid libraryから、ansamitocin生合成に必要な2つのgene clustersをクローニング。 <cluster I> Asm11(480aa) : Oxygenase 配列解析のみ。 ansamitocin形成でC-4/C-5 epoxidationを担うかもしれない。 --- [PMID:14624546](2003) Actinosynnema pretiosumのansamitocin生合成gene clusterにある6genesの機能を、遺伝子不活化とmutantの化学分析によって調査。それらはそれぞれ halogenase (asm12), carbamoyltransferase (asm21), 20-O-methyltransferase (asm7), 3-O-acyltransferase (asm19), epoxidase (asm11), N-methyltransferase (asm10) をコードし、ansamitocin形成において6つのPKS後修飾ステップを担う。 asm11がlarge internal deletionによって不活化された株ではN-demethyl-desepoxy-ansamitocin P-3が蓄積したのに対し、もうひとつのepoxidase候補遺伝子asm30の不活化ではwildレベルのansamitocin P-3が産生された。よって、Asm11が4,5-epoxidaseとして同定された。

Sequence

>epoxidase [oxygenase]
MSRDGVVERVPVLVVGAGPTGLALACGLRSLGVEALVVDRAGGPATTSRALGVQPRGVEV
LLRLGALGDLPGLVIPLREVRMHSEGRLVSVMPMARMAQASGRAPLLVSQVEVEASLRER
LAALGGAPEWGVELLDCAQDASGVTALLRDGGGERRVRADWVVGCDGARSLVRELAGIGF
PGEQVAEHFLIADVRADWAVDRGVMGSWTRGRDMVSVCPLPGEDRWRLMGPAPAGTAGRL
RDDEVRELVLRQLARCTPHPAEAITRVEWLSSFRIHRRLAEAHRRDRLLLAGDAAAIHHP
FGGQGMNTGLGDAENLAWKLALVASGRAGQELLDTYAAERRPVAAGVLSGTGVTTGLVAA
DNPLVALVRDRVVMPLLNTRLAQGFLWRRASQLGVGYRRGPLAAPGARRGSGPVPGDRVP
DLACHDGGGRTTLHAALRGRWAVLAPDGVGGSTVEAVRGLLGVDAVVGLAPVAGGLAEVL
LVRPDGHLAWRGRPDPEGVAAWLGSALSLVGEGALR

>epoxidase [oxygenase]
GTGAGCCGGGACGGGGTGGTCGAGCGGGTCCCGGTGCTGGTGGTCGGCGCGGGGCCGACC
GGGCTGGCGCTGGCGTGCGGGTTGCGCTCGCTCGGGGTGGAGGCCCTGGTGGTCGACCGG
GCGGGCGGTCCCGCGACCACCTCGCGGGCGCTGGGCGTGCAGCCGCGCGGCGTGGAGGTG
CTGCTGCGGCTGGGCGCGCTGGGCGACCTGCCGGGGCTGGTGATCCCGCTGCGCGAGGTG
CGGATGCACAGCGAGGGCAGGCTGGTGTCGGTGATGCCGATGGCGCGCATGGCGCAGGCG
TCGGGGCGCGCGCCGCTGCTGGTGTCCCAGGTGGAGGTGGAGGCGTCGCTGCGGGAGCGG
CTGGCCGCGCTGGGCGGCGCGCCGGAGTGGGGCGTGGAGCTGCTGGACTGCGCGCAGGAC
GCCTCCGGGGTGACCGCGCTGCTGCGCGACGGCGGCGGTGAGCGGCGGGTGCGGGCGGAC
TGGGTGGTCGGGTGCGACGGCGCGCGCAGCCTGGTGCGGGAGCTGGCGGGCATCGGGTTC
CCCGGCGAGCAGGTGGCCGAGCACTTCCTGATCGCCGACGTGCGCGCGGACTGGGCGGTG
GACCGGGGCGTGATGGGCAGCTGGACGCGCGGGCGCGACATGGTCAGCGTGTGCCCGCTT
CCCGGCGAGGACCGGTGGCGGCTCATGGGCCCCGCGCCCGCCGGGACGGCCGGGCGGCTG
CGCGACGACGAGGTGCGGGAGCTGGTGCTGCGCCAGCTCGCGCGCTGCACGCCGCACCCC
GCCGAGGCGATCACGCGGGTGGAGTGGCTGTCGTCGTTCCGGATCCACCGCAGGCTCGCC
GAGGCGCACCGCCGGGACAGGCTGCTGCTGGCCGGGGACGCCGCCGCGATCCACCACCCG
TTCGGCGGGCAGGGCATGAACACCGGGCTGGGCGACGCGGAGAACCTGGCGTGGAAGCTG
GCGCTGGTGGCGAGCGGGCGGGCCGGGCAGGAGCTGCTGGACACCTACGCGGCCGAGCGC
AGGCCCGTCGCGGCGGGGGTGCTCTCGGGGACCGGGGTGACCACCGGCCTGGTCGCGGCG
GACAACCCGCTGGTGGCGCTGGTGCGGGACCGGGTGGTGATGCCGCTGCTGAACACCCGG
CTCGCGCAGGGGTTCCTGTGGCGCAGGGCCTCCCAGCTGGGGGTCGGGTACCGGCGCGGG
CCGCTGGCCGCTCCGGGCGCCCGGCGGGGATCGGGGCCCGTGCCCGGCGACCGGGTGCCC
GACCTGGCGTGCCACGACGGGGGCGGGCGGACGACGCTGCACGCGGCGCTGCGCGGCCGG
TGGGCGGTGCTGGCGCCGGACGGGGTGGGCGGGTCGACCGTGGAAGCGGTGCGGGGGCTG
CTCGGCGTGGACGCCGTGGTGGGGCTGGCGCCGGTGGCGGGCGGGTTGGCGGAGGTGCTG
CTGGTGCGGCCGGACGGGCACCTGGCGTGGCGCGGGCGGCCGGACCCCGAGGGGGTCGCG
GCGTGGCTGGGGTCGGCGCTGAGCCTGGTCGGGGAGGGGGCGCTGCGATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR002938 Monooxygenase, FAD-binding (Domain) PF01494 FAD_binding_3 IPR003042 Aromatic-ring hydroxylase-like (Domain) PR00420 RNGMNOXGNASE
SignalP	No significant hit
TMHMM	No significant hit

Ansam_00120 Ansam_00140